Validation of Housekeeping Genes in the Brains of Rats Submitted to Chronic Intermittent Hypoxia, a Sleep Apnea Model

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dc.contributor.authorJulian, Guilherme Silva [UNIFESP]
dc.contributor.authorOliveira, Renato Watanabe de [UNIFESP]
dc.contributor.authorPerry, Juliana Cini [UNIFESP]
dc.contributor.authorTufik, Sergio [UNIFESP]
dc.contributor.authorChagas, Jair Ribeiro [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:38:00Z
dc.date.available2016-01-24T14:38:00Z
dc.date.issued2014-10-07
dc.description.abstractObstructive sleep apnea (OSA) is a syndrome characterized by intermittent nocturnal hypoxia, sleep fragmentation, hypercapnia and respiratory effort, and it has been associated with several complications, such as diabetes, hypertension and obesity. Quantitative real-time PCR has been performed in previous OSA-related studies; however, these studies were not validated using proper reference genes. We have examined the effects of chronic intermittent hypoxia (CIH), which is an experimental model mainly of cardiovascular consequences of OSA, on reference genes, including beta-actin, beta-2-microglobulin, glyceraldehyde-3-phosphate dehydrogenase, hypoxanthine guanine phosphoribosyl transferase and eukaryotic 18S rRNA, in different areas of the brain. All stability analyses were performed using the geNorm, Normfinder and BestKeeper software programs. With exception of the 18S rRNA, all of the evaluated genes were shown to be stable following CIH exposure. However, gene stability rankings were dependent on the area of the brain that was analyzed and varied according to the software that was used. This study demonstrated that CIH affects various brain structures differently. With the exception of the 18S rRNA, all of the tested genes are suitable for use as housekeeping genes in expression analyses.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Psicobiol, UNIFESP, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biociencias, UNIFESP Baixada Santista, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Psicobiol, UNIFESP, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biociencias, UNIFESP Baixada Santista, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipAssociacao Fundo de Incentivo a Pesquisa (AFIP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDFAPESP: 2011/15060-4
dc.description.sponsorshipIDFAPESP: 2011/16011-6
dc.description.sponsorshipIDCNPq: 558924/2008-5
dc.format.extent8
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0109902
dc.identifier.citationPlos One. San Francisco: Public Library Science, v. 9, n. 10, 8 p., 2014.
dc.identifier.doi10.1371/journal.pone.0109902
dc.identifier.fileWOS000342798000098.pdf
dc.identifier.issn1932-6203
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/38326
dc.identifier.wosWOS:000342798000098
dc.language.isoeng
dc.publisherPublic Library Science
dc.relation.ispartofPlos One
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleValidation of Housekeeping Genes in the Brains of Rats Submitted to Chronic Intermittent Hypoxia, a Sleep Apnea Modelen
dc.typeinfo:eu-repo/semantics/article
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