Effects of low-intensity pulsed ultrasound on injured skeletal muscle
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Data
2013-08-23
Autores
Montalti, Camila Stein [UNIFESP]
Souza, Natália V. C. K. L. [UNIFESP]
Rodrigues, Natália C.
Fernandes, Kelly Rossetti [UNIFESP]
Toma, Renata Luri [UNIFESP]
Renno, Ana Claudia Muniz [UNIFESP]
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BACKGROUND:Low-intensity pulsed ultrasound (LIPUS) has been shown to stimulate tissue metabolism and accelerate muscle healing. However, the optimal parameters in the use of LIPUS are still not clear.OBJECTIVE:The aim of this study was to analyze the effects of LIPUS on muscle healing in rats subjected to a cryolesion.METHOD:Twenty rats were divided into the following groups: an injured control group (CG) and an injured treated group (TG). Both groups were divided into 2 sub-groups (n=5 each) that were sacrificed 7 and 13 days post-surgery. Treatments were started 24 hours after the surgical procedure and consisted of 3 or 6 sessions. After euthanasia, the muscles were submitted to standard histological procedures.RESULTS:Qualitative analyses were based on morphological assessments of the muscle. The histopathological analysis on day 7 revealed that the muscles in the CG and the TG presented an intense inflammatory infiltrate, a large necrotic area and a disorganized tissue structure. After 13 days, both the CG and the TG had granulation tissue and newly formed fibers. The TG presented a more organized tissue structure. The quantitative analysis of collagen indicated similar findings among the groups, although the qualitative analysis revealed a better organization of collagen fibers in the TG at 13 days. The immunohistochemical analysis indicated that, at both time points, the expression of cyclooxygenase-2 was upregulated in the TG compared to the CG.CONCLUSIONS: LIPUS used as a treatment for muscle injury induced a more organized tissue structure at the site of the injury and stimulated the expression of COX-2 and the formation of new muscle fibers.
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Brazilian Journal of Physical Therapy. Associação Brasileira de Pesquisa e Pós-Graduação em Fisioterapia , v. 17, n. 4, p. 343-350, 2013.