IMMUNOCHEMICAL CHARACTERIZATION OF CARBOHYDRATE ANTIGENS FROM FUNGI, PROTOZOA AND MAMMALS BY MONOCLONAL-ANTIBODIES DIRECTED TO GLYCAN EPITOPES
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Data
1995-08-01
Autores
Straus, Anita Hilda [UNIFESP]
Suzuki, E.
Toledo, Marcos Sergio de [UNIFESP]
Takizawa, C. M.
Takahashi, Helio Kiyoshi [UNIFESP]
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Cell surface carbohydrates constitute the major antigenic determinants of fungi and protozoa. Glycoconjugates also represent a large variety of antigens or markers present in mammals such as histo-blood groups ABO, differentiation and heterophile antigens, among others. This article focuses on the general properties of glycoconjugate antigens and production and characterization of the anti-carbohydrate monoclonal antibodies (MoAbs). It describes the specificity and some properties of monoclonal antibodies directed against carbohydrate epitopes present in tumor-associated glycoproteins, in glycosaminoglycans of higher eukaryotes and in glycolipid antigens of protozoa and fungi. The epitopes recognized by the anti-carbohydrate MoAbs range from one sugar unit up to ten sugar units. Although most anti-carbohydrate MoAbs are directed predominantly toward terminal sugar residues, a few MoAbs are also reactive with internal sugar residues. The fine structure of the carbohydrate epitopes has been chemically defined by [H-1] NMR, GC/MS of alditol acetates of partially permethylated compounds, (-)FAB/MS, degradation with exoglycosidases and inhibition with different methyl-glycosides and oligosaccharides.
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Brazilian Journal Of Medical And Biological Research. Sao Paulo: Assoc Bras Divulg Cientifica, v. 28, n. 8, p. 919-923, 1995.