Efeitos da Prostaglandina E1 na gênese de capilares sanguíneos em músculo esquelético isquêmico de ratos: estudo histológico e ultra-estrutural
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Data
2006
Autores
Moreschi Junior, Dorival [UNIFESP]
Orientadores
Fagundes, Djalma José [UNIFESP]
Tipo
Tese de doutorado
Título da Revista
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Objetivo: o objetivo deste trabalho foi estudar os aspectos morfológicos e ultra-estruturais na
gênese de capilares sanguíneos em músculo esquelético do membro caudal de ratos
submetidos à isquemia sob a ação da Prostaglandina E1 (PGE1), administrada por via
intramuscular ou endovenosa. Métodos: foram utilizados 60 ratos (Rattus norvegicus
albinus), linhagem Wistar-UEM, distribuídos aleatoriamente em três grupos de 20,
redistribuídos igualmente em dois subgrupos, observados no 7o
e 14o
dias, sendo um grupo
controle onde apenas foi provocada a isquemia no membro, outro com a isquemia e a injeção
da PGE1 via intramuscular (IM), e outro com a isquemia e a injeção da PGE1 endovenosa
(EV). Para análise dos resultados, foram realizadas a coloração com hematoxilina & eosina
(HE), a imuno-histoquímica e a microscopia eletrônica de transmissão (MET). Resultados:
constatou-se um aumento estatisticamente significante no número de capilares nos subgrupos
com o uso da PGE1 IM e EV, através da contagem nos cortes corados com HE. Houve
marcação de capilares e vasos de maior calibre nestes mesmos subgrupos, porém, esta reação
não foi eficiente para a quantificação dos capilares. Na MET encontraram-se evidências de
formação de novos capilares. Conclusões: a PGE1, administrada por via IM ou EV,
promoveu, após 14 dias de observação, um aumento no número de capilares no músculo
esquelético de ratos submetido à isquemia, identificáveis histologicamente com a coloração
em HE. Na análise ultra-estrutural encontraram-se alterações que sugerem, nos animais sob a
ação da PGE1, que a neoformação vascular possa ter ocorrido por angiogênese e
vasculogênese. A imuno-coloração, apesar da marcação de capilares e vasos maiores, não
permitiu estabelecer uma correlação com o aumento de vasos encontrados na coloração com
HE.
Objective: The objective of this work was to study the morphologic and ultra-structural aspects in the genesis of blood capillaries in the lower limb skeletal muscle of rats submitted to ischemia under the action of intramuscular or endovenous Prostaglandin E1 (PGE1). Methods: Sixty Wistar-UEM rats (Rattus norvegicus albinus) were used, randomly distributed into three groups of 20, equally redistributed into two subgroups, observed at the 7th and 14th days as follows: group only with ischemia was considered as control (C), group with ischemia and intramuscular injection of PGE1 (IM), and group with ischemia and endovenous injection of the PGE1 (EV). The analysis of results was performed with the HE staining, imuno-histochemistry and transmission electronic microscopy (TEM). Results: an increase statistically significant was verified in the number of capillaries in the subgroups with PGE1 using IM or EV, through the counting in the samples with HE staining. Demarcation of capillaries and larger vases in these subgroups were observed, however, this reaction was not efficient for the quantification of the capillaries. In the electronic microscopy, evidences of new capillary formation were found. Conclusions: intramuscular or endovenous PGE1 promoted an increase on the number of capillaries in the skeletal muscle of rats submitted to ischemia after 14 days of observation histologically identifiable through HE staining. In the ultra-structural analysis, alterations found suggest, for animals under the action of PGE1 that the vascular neoformation might have occurred through angiogenesis and/or vasculogenesis. The imuno-staining, despite the demarcation of capillary and larger vases, did not allow the establishment of a correlation with the increase of vases found in the HE staining.
Objective: The objective of this work was to study the morphologic and ultra-structural aspects in the genesis of blood capillaries in the lower limb skeletal muscle of rats submitted to ischemia under the action of intramuscular or endovenous Prostaglandin E1 (PGE1). Methods: Sixty Wistar-UEM rats (Rattus norvegicus albinus) were used, randomly distributed into three groups of 20, equally redistributed into two subgroups, observed at the 7th and 14th days as follows: group only with ischemia was considered as control (C), group with ischemia and intramuscular injection of PGE1 (IM), and group with ischemia and endovenous injection of the PGE1 (EV). The analysis of results was performed with the HE staining, imuno-histochemistry and transmission electronic microscopy (TEM). Results: an increase statistically significant was verified in the number of capillaries in the subgroups with PGE1 using IM or EV, through the counting in the samples with HE staining. Demarcation of capillaries and larger vases in these subgroups were observed, however, this reaction was not efficient for the quantification of the capillaries. In the electronic microscopy, evidences of new capillary formation were found. Conclusions: intramuscular or endovenous PGE1 promoted an increase on the number of capillaries in the skeletal muscle of rats submitted to ischemia after 14 days of observation histologically identifiable through HE staining. In the ultra-structural analysis, alterations found suggest, for animals under the action of PGE1 that the vascular neoformation might have occurred through angiogenesis and/or vasculogenesis. The imuno-staining, despite the demarcation of capillary and larger vases, did not allow the establishment of a correlation with the increase of vases found in the HE staining.
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Citação
MORESCHI JUNIOR, Dorival. Efeitos da Prostaglandina E1 na gênese de capilares sanguíneos em músculo esquelético isquêmico de ratos: estudo histológico e ultra-estrutural. 2006. 66 f. Tese (Doutorado em Ciências) - Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, 2006.