Association between Cationic Liposomes and Low Molecular Weight Hyaluronic Acid
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2015-03-24
Autores
Gasperini, Antonio A. M.
Puentes-Martinez, Ximena E. [UNIFESP]
Balbino, Tiago Albertini
Rigoletto, Thais de Paula
Cavalcanti Correa, Gabriela de Sa
Cassago, Alexandre
Portugal, Rodrigo Villares
La Torre, Lucimara Gaziola de
Cavalcanti, Leide P.
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This work presents a study of the association between low molecular weight hyaluronic acid (16 kDa HA) and cationic liposomes composed of egg phosphatidylcholine (EPC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), and 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP). the cationic liposome/HA complexes were evaluated to determine their mesoscopic structure, average size, zeta potential, and morphology as a function of the amount of HA in the system. Small angle X-ray scattering results revealed that neighboring cationic liposomes either stick together after a partial coating of low concentration HA or disperse completely in excess of HA, but they never assemble as multilamellar vesicles. Cryo-transmission electron microscopy images confirm the existence of unilamellar vesicles and large aggregates of unilamellar vesicles for HA fractions up to 80% (w/w). High concentrations of HA (> 20% w/w) proved to be efficient for coating extruded liposomes, leading to particle complexes with sizes in the nanoscale range and a negative zeta potential.
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Langmuir. Washington: Amer Chemical Soc, v. 31, n. 11, p. 3308-3317, 2015.