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dc.contributor.authorDi Cesare, Sebastian
dc.contributor.authorNantel, Andre
dc.contributor.authorMarshall, Jean-Claude
dc.contributor.authorFernandes, Bruno F. [UNIFESP]
dc.contributor.authorAntecka, Emilia
dc.contributor.authorOrellana, Maria E.
dc.contributor.authorAbourbih, Daniel
dc.contributor.authorSaornil, Antonia M.
dc.contributor.authorBurnier, Miguel N. [UNIFESP]
dc.date.accessioned2016-01-24T13:59:30Z
dc.date.available2016-01-24T13:59:30Z
dc.date.issued2010-04-01
dc.identifierhttp://dx.doi.org/10.1007/s00432-009-0692-3
dc.identifier.citationJournal of Cancer Research and Clinical Oncology. New York: Springer, v. 136, n. 4, p. 577-586, 2010.
dc.identifier.issn0171-5216
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/32408
dc.description.abstractFresh biopsied ocular tumor tissues are difficult to obtain for the purpose of performing microarray experiments on extracted nucleic acids. Present technology allows for extraction of total RNA from formalin-fixed paraffin embedded (FFPE) tissue analyzed by the cDNA mediated Annealing Sectioning and Ligation (DASL) method. We aimed to correlate gene transcript differences between two uveal melanoma (UM) clinical-histopathological parameters (metastasis, cell type).A total of 43 FFPE UM were used. the expression of RPL13a, a ribosomal protein gene, for each sample was used to evaluate the quality of RNA extracted from FFPE tissue. Gene expression values generated from the array were analyzed using the GeneSpring GX software (Agilent). Immunohistochemistry was used in order to validate transcriptional findings at the protein level.A total of 106 genes were identified with (P < 0.05, Welch ANOVA test) a difference in transcript abundance for the metastasis clinical parameter. Furthermore, we identified 64 genes with a statistically significant (P < 0.05) difference in transcript abundance between the spindle and epithelioid cell types. Each individual sample for both groups (metastasis, cell type) exhibited distinct transcriptional profiles that were separated on a PCA. Positive nuclear immunostaining for LIG4-metastasis, ErbB3-cell type was found to be associated with better patient prognosis and outcome.To the best of our knowledge, this is the first time that a successful retrospective analysis has been done with UM FFPE RNA. This data may lead to future customized therapeutic targets, which may improve the now unchanged mortality rate of this particular malignancy.en
dc.description.sponsorshipCedars Cancer Institute
dc.description.sponsorshipHellen Keller Foundation
dc.format.extent577-586
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofJournal of Cancer Research and Clinical Oncology
dc.rightsAcesso restrito
dc.subjectUveal melanomaen
dc.subjectFFPEen
dc.subjectDASLen
dc.subjectExpression profilingen
dc.subjectHistopathologyen
dc.subjectClinical parametersen
dc.titleExpression profiling of formalin-fixed paraffin embedded primary human uveal melanomas using DASL matricesen
dc.typeArtigo
dc.rights.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.contributor.institutionMcGill Univ
dc.contributor.institutionNatl Res Council Canada
dc.contributor.institutionNCI
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Valladolid
dc.description.affiliationMcGill Univ, Ctr Hlth, Henry C Witelson Ophthalm Pathol Lab, Montreal, PQ H3A 2B4, Canada
dc.description.affiliationMcGill Univ, Ctr Hlth, Henry C Witelson Ophthalm Pathol Registry, Montreal, PQ H3A 2B4, Canada
dc.description.affiliationNatl Res Council Canada, Biotechnol Res Inst, Montreal, PQ H4P 2R2, Canada
dc.description.affiliationNCI, NIH, Bethesda, MD 20892 USA
dc.description.affiliationUniversidade Federal de São Paulo, UNIFESP EPM, Dept Ophthalmol, São Paulo, Brazil
dc.description.affiliationUniv Valladolid, Dept Ophthalmol, Ocular Pathol Lab, Valladolid, Spain
dc.description.affiliationUnifespUniversidade Federal de São Paulo, UNIFESP EPM, Dept Ophthalmol, São Paulo, Brazil
dc.identifier.doi10.1007/s00432-009-0692-3
dc.description.sourceWeb of Science
dc.identifier.wosWOS:000274686800012


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