Expression of fibroblast growth factor-8 and its cognate receptors, fibroblast growth factor receptor (FGFR)-3c and-4, in fetal bovine preantral follicles

Expression of fibroblast growth factor-8 and its cognate receptors, fibroblast growth factor receptor (FGFR)-3c and-4, in fetal bovine preantral follicles

Author Buratini, J. Google Scholar
Glapinski, V. F. Google Scholar
Giometti, I. C. Google Scholar
Teixeira, A. B. Google Scholar
Costa, I. B. Google Scholar
Avellar, MCW Google Scholar
Barros, C. M. Google Scholar
Price, C. A. Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Univ Montreal
Abstract Paracrine cell signaling is thought to be important for ovarian follicle development, and a role for some members of the fibroblast growth factor (FGF) family have been suggested. in the present study, we tested the hypothesis that FGF-8 and its cognate receptors (FGFR-3c and FGFR-4) are expressed in bovine preantral follicles. Reverse transcription-polymerase chain reaction was used to amplify bovine FGF-8, FGFR-3c, and FGFR-4 from preantral follicle samples and a variety of fetal and adult tissues. All three genes were widely expressed in fetal tissues, with a restricted expression pattern in adult tissues. FGF-8 and FGFR-3c were expressed in secondary follicles in 70% of fetuses examined, whereas FGFR-4 expression was significantly less frequent (20%). FGFR-3c expression frequency was significantly lower in primordial compared to secondary follicles, and FGF-8 expression showed a similar trend. FGFR-4 was only observed when all follicle classes of an individual were expressing both FGF-8 and FGFR-3c. We conclude that FGF-8 and its receptors are expressed in preantral follicles in a developmentally regulated manner. (C) 2005 Wiley-Liss, Inc.
Keywords fibroblast growth factor-8
fibroblast growth factor receptor-3c
fibroblast growth factor receptor-4
preantral follicle
ovary
Language English
Date 2005-03-01
Published in Molecular Reproduction and Development. Hoboken: Wiley-liss, v. 70, n. 3, p. 255-261, 2005.
ISSN 1040-452X (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 255-261
Origin http://dx.doi.org/10.1002/mrd.20205
Access rights Closed access
Type Article
Web of Science ID WOS:000226695700002
URI http://repositorio.unifesp.br/handle/11600/28169

Show full item record




File

File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Search


Browse

Statistics

My Account