Navegando por Palavras-chave "species identification"

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    Multicenter evaluation of mycobacteria identification by PCR restriction enzyme analysis in laboratories from Latin America and the Caribbean
    (Elsevier B.V., 2005-05-01) Leao, S. C.; Bernardelli, A.; Cataldi, A.; Zumarraga, M.; Robledo, J.; Realpe, T.; Mejia, G. I.; Telles, MAD; Chimara, E.; Velazco, M.; Fernandez, J.; Rodrigues, P. A.; Guerrero, M. I.; Leon, C. I.; Porras, T. B.; Rastogi, N.; Goh, K. S.; Suffys, P.; Rocha, A. D.; Netto, D. D.; Ritacco, V; Lopez, B.; Barrera, L.; Palomino, J. C.; Martin, A.; Portaels, F.; Universidade Federal de São Paulo (UNIFESP); SENASA; INTA; Corp Invest Biol; Univ Pontificia Bolivariana; Inst Adolfo Lutz Registro; Inst Salud Publ Chile; Inst Nacl Salud; Inst Pasteur Guadeloupe; Fdn Oswaldo Cruz; Inst Nacl Enfermedades Infecciosas Carlos Malbran; Inst Trop Med
    The identification of mycobacterial species in clinical isolates is essential for making patient care decisions. Polymerase chain reaction (PCR) restriction enzyme analysis (PRA) is a simple and rapid identification method, based on amplification of 441 bp of the hsp65 gene and restriction with BstEII and HaeIII. As a contribution to the validation of PRA, a multicenter study was performed in eight laboratories located in Argentina, Brazil, Colombia, Chile, and Guadeloupe. Each laboratory received 18 coded isolates from the collection of the Institute of Tropical Medicine (Antwerp, Belgium), representing duplicates of nine laboratory strains: Mycobacterium terrae CIPT 140320001, Mycobacterium scrofulaceum CIPT 140220031, Mycobacterium flavescens ATCC 14474, Mycobacterium triviale ATCC 23292, Mycobacterium nonchromogenicum ATCC 19530, Mycobacterium chitae ATCC 19627, Mycobacterium abscessus ATCC 19977, Mycobacterium kansasii ATCC 12478, and Mycobacterium peregrinum ATCC 14467. A detailed protocol including amplification, enzymatic digestion, and gel preparation was provided to each laboratory. Two laboratories identified correctly all 18 (100%) isolates, one identified correctly 17 (94.5%), two identified 14 (77.7%), one identified 11 (61%), and two identified 8 (44.4%) isolates. Errors detected in laboratories with more than 77% accuracy were associated with electrophoresis running conditions and an unspecific amplicon produced by a single strain. Lower accuracy was mainly related to inappropriate use of DNA markers and insufficient training in interpretation of patterns. in conclusion, the PRA method was readily implemented in some Latin American and Caribbean laboratories of mycobacteria, but improvements in critical points, as gel running conditions and training in interpretation of patterns, are needed in order to improve accuracy. in others, improvement in critical points is still necessary. (C) 2004 Elsevier B.V. All rights reserved.
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    Species diversity, antifungal susceptibility and phenotypic and genotypic characterisation of Exophiala spp. infecting patients in different medical centres in Brazil
    (Wiley, 2017) Silva, Wendy C. [UNIFESP]; Goncalves, Sarah S.; Santos, Daniel W. C. L. [UNIFESP]; Padovan, Ana Carolina B. [UNIFESP]; Bizerra, Fernando C. [UNIFESP]; Melo, Analy S. A. [UNIFESP]
    The Exophiala genus is responsible for many superficial and invasive infections resulting from black fungi. Identification of Exophiala at the species level is based on morphological observations complemented by molecular tests. The aim of this study was to identify 23 clinical isolates of Exophiala spp. and evaluate the antifungal susceptibility to seven different agents. Molecular identification was based on an analysis of ITS region of rDNA using genomic databases. The micromorphology was evaluated by microculture and scanning electron microscopy. The susceptibility tests were performed using the antifungal agents 5-fluorocytosine (5-FC), amphotericin B (AMB), itraconazole (ITC), voriconazole (VRC), posaconazole (PSC), caspofungin (CFG) and terbinafine (TRB). The ITS analysis identified 100% of the following isolates as: E.dermatitidis (8), E.xenobiotica (6), E.bergeri (4), E.oligosperma (3), E.spinifera (1) and E.mesophila (1). The antifungal susceptibility tests showed that the triazoles compounds were in vitro the most active agents against Exophiala. ITS sequencing enabled the accurate identification of the 23 tested isolates. The triazoles, particularly itraconazole and posaconazole, exhibited MIC values lower than AMB, CAS and 5-FC. Although the guidelines do not indicate AMB for treatment against Exophiala spp., this study showed activity for all of the tested species, except E.mesophila.