Navegando por Palavras-chave "reverse-hybridisation assay"

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    Performance of a molecular assay to detect Mycobacterium tuberculosis complex DNA in clinical specimens: multicenter study in Brazil
    (Fundaco Oswaldo Cruz, 2017) Verza, Mirela; Schmid, Karen Barros; Barcellos, Regina Bones; Linck, Natali; Bello, Graziele Lima; Scapin, Daniel; Sperhacke, Rosa Dea; Nunes Silva, Marcia Susana; Wollheim, Claudia; Celle Rivero, Martha Gabriela [UNIFESP]; Kritski, Afranio Lineu; Rezende, Leonides, Jr.; Oliveira, Martha Maria; Dalla Costa, Elis Regina; Rosa Rossetti, Maria Lucia
    BACKGROUND In high tuberculosis (TB) burden countries, there are few data on the performance of new molecular commercialised assays developed locally. OBJECTIVE To evaluate the performance of a new molecular commercialised assay for TB diagnosis (Detect-TB) in three laboratories. METHODS A total of 302 sputum samples from an equal number of patients with presumptive diagnosis of pulmonary tuberculosis (PTB) were submitted for routine smear microscopy, culture, and Detect-TB assay at three different sites in Brazil (the cities of Caxias do Sul, Sao Paulo and Canoas). FINDINGS Seventy four (24.7%) TB cases were diagnosed (65 bacteriologically confirmed). When compared to smear microscopy/ culture results, the overall sensitivity and specificity of Detect-TB assay was 84.6% (CI 95%