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    Efeitos da oxigenação hiperbárica sobre a morfologia e a quantificação do colágeno em retalhos cutâneos randômicos de ratos
    (Universidade Federal de São Paulo (UNIFESP), 2016-02-26) Rech, Fabricio Valandro [UNIFESP]; Fagundes, Djalma Jose [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Objective. Evaluate the microscopic morphology, necrotic area and the collagen content in the rat skin flaps submitted to hyperbaric oxygen therapy (HBOT). Methods. Forty rats adults were divided into four groups: GE ? hair removal; GE / OHB ? hair removal undergone HBO; GER ? hair removal and will perform a skin flap (2 cm wide by 8 cm in length on the back) and GER / OHB ? hair removal, and performed skin flap and submitted to OHB. OHB (2.4 ATA) was performed for two hours, for seven consecutive days. On the eighth day the animals were anesthetized, the flaps removed and separated into three parts: the proximal, middle and distal - with respect to the pedicle fixation. The material was fixed in 10% formalin and processed for paraffin embedding, and the sections stained by HE and picrosirius-red. The blades in HE were examined under a light microscope and collagen content valued at polarizing microscope and ImageLab® software (Bio-Rad). Results. The macroscopic evaluation showed a higher area of necrosis in the three regions of the GER group regarding GE / HBO: (Proximal = 24.6 ± 10.6> 5.6 ± 1.6; Medial; 84.5 ± 5.9> 7.5 ± 3.7; Distal: 98.5 ± 3.7> 15.4 ± 2.5) compared to the GE / OHB. The microscopy study showed a major histopathological lesions in the three regions of the GER group compared to the GE / OHB (Proximal - 8.6 ± 1.2> GER / OHB = 2.4 ± 0.6; medial = 11.5 ± 2. 7> 5.4 ± 1.9 and 14.8 ± 5.3 distal => 8.1 ± 3.7. The collagen concentration was higher in the medial and distal region in GER / OHB group compared to GER (Medial: 81.5 ± 16.7> 55.5 ± 12.6; Distal: 72.2 ± 16.7> 32.8 ± 12.5). Conclusion. Hyperbaric oxygenation had favorable effects on random skin flaps in rats considering the microscopic morphology, necrotic area and collagen concentration.