Navegando por Palavras-chave "oxigenação hiperbárica"

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    Efeito da oxigenação hiperbárica em lesão ototóxica produzida pela amicacina em cobaias
    (Associação Brasileira de Otorrinolaringologia e Cirurgia Cervicofacial, 2013-06-01) Amora, Luciana de Albuquerque Salviano; Murashima, Adriana de Andrade Batista; Rossato, Maria; Moreira, Márcia Bento; Hyppolito, Miguel Ângelo; Fagundes, Djalma José [UNIFESP]; Universidade de São Paulo (USP); Universidade Federal do Vale do São Francisco Colegiado de Medicina Veterinária; Universidade Federal de São Paulo (UNIFESP)
    Hyperbaric oxygen therapy (HBOT) has enhanced the prevention and treatment of auditory ailments such as ototoxicity. OBJECTIVE: To study the effects of HBOT upon ototoxic injuries produced by amikacin. METHOD: This experimental study included 12 albino guinea pigs, whose auditory function was assessed through distortion product otoacoustic emissions (DPOAEs) and brainstem auditory evoked potentials (BAEPs) before and after the administration of amikacin (600 mg/kg/day) and HBOT sessions (2 ATA, 60 minutes). Morphological features were analyzed through scanning electron microscopy. Subjects were divided into four groups, as follows: group 1 - saline solution + HBOT; group 2 - amikacin for 8 days; group 3 - amikacin + seven days of rest; and group 4 - amikacin + HBOT. RESULTS: Group 1 subjects had preserved function and morphology throughout the experiment; Group 2 subjects had statistically significant levels of hair cell injury and functional impairment; Subjects on groups 3 and 4 had statistically significant functional and morphological impairment after the administration of amikacin, which were still present after the proposed procedures had been carried out. CONCLUSION: Hyperbaric oxygen therapy did not change the cochlear hair cell morphology or the electro-physiological thresholds of the guinea pigs given amikacin.
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    Efeitos da oxigenação hiperbárica sobre a morfologia e a quantificação do colágeno em retalhos cutâneos randômicos de ratos
    (Universidade Federal de São Paulo (UNIFESP), 2016-02-26) Rech, Fabricio Valandro [UNIFESP]; Fagundes, Djalma Jose [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Objective. Evaluate the microscopic morphology, necrotic area and the collagen content in the rat skin flaps submitted to hyperbaric oxygen therapy (HBOT). Methods. Forty rats adults were divided into four groups: GE ? hair removal; GE / OHB ? hair removal undergone HBO; GER ? hair removal and will perform a skin flap (2 cm wide by 8 cm in length on the back) and GER / OHB ? hair removal, and performed skin flap and submitted to OHB. OHB (2.4 ATA) was performed for two hours, for seven consecutive days. On the eighth day the animals were anesthetized, the flaps removed and separated into three parts: the proximal, middle and distal - with respect to the pedicle fixation. The material was fixed in 10% formalin and processed for paraffin embedding, and the sections stained by HE and picrosirius-red. The blades in HE were examined under a light microscope and collagen content valued at polarizing microscope and ImageLab® software (Bio-Rad). Results. The macroscopic evaluation showed a higher area of necrosis in the three regions of the GER group regarding GE / HBO: (Proximal = 24.6 ± 10.6> 5.6 ± 1.6; Medial; 84.5 ± 5.9> 7.5 ± 3.7; Distal: 98.5 ± 3.7> 15.4 ± 2.5) compared to the GE / OHB. The microscopy study showed a major histopathological lesions in the three regions of the GER group compared to the GE / OHB (Proximal - 8.6 ± 1.2> GER / OHB = 2.4 ± 0.6; medial = 11.5 ± 2. 7> 5.4 ± 1.9 and 14.8 ± 5.3 distal => 8.1 ± 3.7. The collagen concentration was higher in the medial and distal region in GER / OHB group compared to GER (Medial: 81.5 ± 16.7> 55.5 ± 12.6; Distal: 72.2 ± 16.7> 32.8 ± 12.5). Conclusion. Hyperbaric oxygenation had favorable effects on random skin flaps in rats considering the microscopic morphology, necrotic area and collagen concentration.