Navegando por Palavras-chave "nanotechnology"
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- ItemSomente MetadadadosIn Vitro Evaluation of Chloroaluminum Phthalocyanine Nanoemulsion and Low-Level Laser Therapy on Human Skin Dermal Equivalents and Bone Marrow Mesenchymal Stem Cells(Bentham Science Publ Ltd, 2011-08-01) Primo, F. L.; Reis, M. B. da Costa [UNIFESP]; Porcionatto, Marimélia Aparecida [UNIFESP]; Tedesco, Antonio Claudio [UNIFESP]; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP)Nanotechnology and tissue engineering are promising scientific fields in the development of advanced materials useful to human health. This article describes the preparation of a nanocarrier for the controlled release of a photosensitizer compound associated with low-level light therapy for skin wound healing treatment and applicable to other skin diseases. A biological model was used as an in vitro skin equivalent based on a three-dimensional culture of fibroblasts and mesenchymal stem cells and denominated by dermal equivalent (DE). Results show that it is possible to use the photomodulation process to control the wound healing in a scratching process and to induce the biomolecules release, both of which are related with the inflammatory wound healing process. In the studies, the MMP-2 and MMP-9 expression from zymography analyses were evaluated. All results showed a dependence on enzymatic activity relating to low-level laser applications which indicates a potential application in wound healing processes based on phototherapy and nanotechnology.
- ItemSomente MetadadadosNanotechnology in Phytotherapy: Antiinflammatory Effect of a Nanostructured Thymol Gel from Lippia sidoides in Acute Periodontitis in Rats(Wiley-Blackwell, 2016) Botelho, Marco A.; Barros, Gisele; Queiroz, Dinalva B.; Carvalho, Celso Felicio; Gouvea, Julia; Patrus, Lia; Bannet, Mariane; Patrus, Danile; Rego, Amalia; Silva, Ivaldo [UNIFESP]; Campus, Guglielmo; Araujo-Filho, IramiLippia sidoides Cham (Verbenaceae) is largely distributed in the northeastern region of Brazil. It is popularly known as Alecrim-pimenta '. Recent studies have shown that some species of Lippia have interesting pharmacological activities. This study aimed to evaluate the effect of a nanostructured thymol gel (TG) 1.2mg/g on acute phase of ligature-induced periodontitis model [acute periodontal disease (APD)] in rats. APD was induced in 24 Wistar rats subjected to ligature placement on left molars in maxillae. Animals were treated with TG, immediately after APD induction. Saline-based gel was utilized as negative control and diethylammonium diclofenac gel 10mg/g was used as positive control. Animals were randomly assigned into the groups. The periodontium and the surrounding gingiva were examined at histopathology, as well as the neutrophil influx into the gingiva was assayed using myeloperoxidase activity levels by ELISA method. TG treatment reduced tissue lesion at histopathology coupled to decreased myeloperoxidase activity production in gingival tissue when compared with the saline gel control group (p < 0.05). The TG gel was able to provide a significant myeloperoxidase decreasing in gingiva tissue confirming to be effective in reducing gingival inflammation in this model. Copyright (c) 2015 John Wiley & Sons, Ltd.
- ItemSomente MetadadadosNanotechnology in Phytotherapy: Antiinflammatory Effect of a Nanostructured Thymol Gel from Lippia sidoides in Acute Periodontitis in Rats(Wiley-Blackwell, 2016) Botelho, Marco A.; Barros, Gisele; Queiroz, Dinalva B.; Carvalho, Celso Felicio; Gouvea, Julia; Patrus, Lia; Bannet, Mariane; Patrus, Danile; Rego, Amalia; Silva, Ivaldo [UNIFESP]; Campus, Guglielmo; Araujo-Filho, IramiLippia sidoides Cham (Verbenaceae) is largely distributed in the northeastern region of Brazil. It is popularly known as Alecrim-pimenta '. Recent studies have shown that some species of Lippia have interesting pharmacological activities. This study aimed to evaluate the effect of a nanostructured thymol gel (TG) 1.2mg/g on acute phase of ligature-induced periodontitis model [acute periodontal disease (APD)] in rats. APD was induced in 24 Wistar rats subjected to ligature placement on left molars in maxillae. Animals were treated with TG, immediately after APD induction. Saline-based gel was utilized as negative control and diethylammonium diclofenac gel 10mg/g was used as positive control. Animals were randomly assigned into the groups. The periodontium and the surrounding gingiva were examined at histopathology, as well as the neutrophil influx into the gingiva was assayed using myeloperoxidase activity levels by ELISA method. TG treatment reduced tissue lesion at histopathology coupled to decreased myeloperoxidase activity production in gingival tissue when compared with the saline gel control group (p < 0.05). The TG gel was able to provide a significant myeloperoxidase decreasing in gingiva tissue confirming to be effective in reducing gingival inflammation in this model. Copyright (c) 2015 John Wiley & Sons, Ltd.
- ItemSomente MetadadadosSilver nanoparticles: a brief review of cytotoxicity and genotoxicity of chemically and biogenically synthesized nanoparticles(Wiley-Blackwell, 2012-11-01) Lima, Renata de; Seabra, Amedea B. [UNIFESP]; Duran, Nelson; Univ Sorocaba; Universidade Federal de São Carlos (UFSCar); Universidade Federal de São Paulo (UNIFESP); Universidade Federal do ABC (UFABC); Universidade Estadual de Campinas (UNICAMP)In recent years interest in silver nanoparticles and their applications has increased mainly because of the important antimicrobial activities of these nanomaterials, allowing their use in several industrial sectors. However, together with these applications, there is increasing concerning related to the biological impacts of the use of silver nanoparticles on a large scale, and the possible risks to the environment and health. in this scenario, some recent studies have been published based on the investigation of potential inflammatory effects and diverse cellular impacts of silver nanoparticles. Another important issue related to nanoparticle toxicity in biological media is the capacity for increased damage to the genetic material, since nanoparticles are able to cross cell membranes and reach the cellular nucleus. in this regard, there is increasing interest in the analysis of potential nanoparticle genotoxicity, including the effects of different nanoparticle sizes and methods of synthesis. However, little is known about the genotoxicity of different silver nanoparticles and their effects on the DNA of organisms; thus further studies in this field are required. This mini-review aims to present and to discuss recent publications related to genotoxicity and the cytotoxicity of silver nanoparticles in order to better understand the possible applications of these nanomaterials in a safe manner. This present work concludes that biogenic silver nanoparticles are generally less cyto/genotoxic in vivo compared with chemically synthesized nanoparticles. Furthermore, human cells were found to have a greater resistance to the toxic effects of silver nanoparticles in comparison with other organisms. Copyright (c) 2012 John Wiley & Sons, Ltd.
- ItemSomente MetadadadosStrategies for Development of Antimalarials Based on Encapsulated Porphyrin Derivatives(Bentham Science Publ Ltd, 2014-01-01) Deda, Daiana Kotra; Budu, Alexandre [UNIFESP]; Cruz, Laura Nogueira; Araki, Koiti; Garcia, Celia Regina da Silva; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP)Despite the efforts in controlling the parasite and infection, and the significant progress achieved in recent years in its treatment, malaria is still prevalent in many regions and out of control in others. The repertoire of alternatives to fight malaria is being expanded, not only by designing new drugs but also by developing improved drug delivery systems able to enhance the antimalarial efficiency of conventional and new drugs. Among the new drugs that have been investigated, several publications report the use of porphyrin derivatives as antimalarials but their efficiency is contradictory. The low activity of porphyrins seems to be associated with low dispersibility and bioavailability. In this respect, Nanotechnology can provide efficient solutions to enhance bioavailability and delivery of conventional and new antimalarials, in order to assure high enough efficiency levels to inactivate the parasite. Thus, in this review we highlight the use of drug delivery systems for conventional and new antimalarials and we propose the encapsulation of porphyrins as a promising alternative for development of anti-malarial formulations.