Navegando por Palavras-chave "myometrium"
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- ItemSomente MetadadadosEffect of estrogen on intracellular signaling pathways linked to activation of M-2- and M-3-muscarinic acetylcholine receptors in the rat myometrium(Elsevier B.V., 2000-02-25) Abdalla, Fernando M. F.; Abreu, Lygia C. [UNIFESP]; Porto, Catarina S. [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Inst ButantanThe estrogen treatment of adult female rats induces an increase in myometrium sensitivity to cholinergic agonists and in this tissue the presence of M-2- and M-3-muscarinic acetylcholine (mACh) receptor was shown. We now report the effect of estrogen on intracellular signaling pathways linked to activation of M-2- and M-3-mACh receptor subtypes. the intracellular cyclic AMP accumulation and [H-3]-inositol phosphates content were measured in myometrium strips from rats in estrus (control) and estradiol-treated rats (12.5 mu g/100 g body weight, sc, 24 h before experiments) (the plasma estradiol level was 30.9 +/- 3.5 pg/ml and 119.3 +/- 14.1 pg/ml from control and estrogen-treated rats, respectively). Estrogen treatment increased 2.5-fold the intracellular cyclic AMP accumulation induced by 10 mu M forskolin. the effects of muscarinic agonist and antagonists on cyclic AMP accumulation were tested. Carbachol reduced the forskolin-induced intracellular cyclic AMP content, 3.0 and 10.5-fold, in myometrium from control and estradiol-treated rats, respectively. This inhibitory effect failed to occur when carbachol was incubated in the presence of methoctramine. Carbachol also induced increase on total [H-3]-inositol phosphates accumulation in myometrium from estradiol-treated rats when compared with control rats. This effect was reversed by pfHHSiD. These studies suggest the modulation by estrogen of intracellular signaling pathways linked to activation of M-2- and M-3-mACh receptors in the rat myometrium. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.
- ItemSomente MetadadadosEffect of estrogen on muscarinic acetylcholine receptor expression in rat myometrium(Elsevier B.V., 2004-01-15) Abdalla, FMF; Marostica, E.; Picarelli, Z. P.; Abreu, L. C.; Avellar, MCW; Porto, C. S.; Universidade Federal de São Paulo (UNIFESP); Inst ButantanWe report the effect of acute estrogen treatment in the expression of muscarinic acetylcholine receptors (mAChRs) in myometrium. Strips were obtained from rats in estrus (control) and treated with estrogen, 24 It before the experiments. Reverse transcriptase-polymerase chain reaction (RT-PCR) was performed and m2, m3 and m5 mAChR mRNA subtypes were detected in myometrium from both groups. [H-3]Quinuclidinyl benzilate ([(3)HQNB]) binding studies indicated that estrogen treatment did not change the affinity and density of mAChRs in myometrial membranes. Displacement curves of [(3)HQNB] with different mAChRs antagonists indicated a one-site fit for all antagonists tested. Comparison of pK(i) values indicated a significant correlation to M-2-mAChR subtype. Functional studies, however, showed that estrogen treatment increased myometrium sensitivity to carbachol and the calculated apparent affinity values were significantly correlated to M-3-mAChR. Furthermore, the pharmacological profile of the two populations of mAChR was not affected by estrogen. in conclusion, these results provide evidence for the presence of M-2- and M-3-mAChR, at the mRNA and protein level, in the rat myometrium and indicate that estrogen induces an increase in myometrial responsiveness to mAChR agonists. (C) 2003 Elsevier Ireland Ltd. All rights reserved.
- ItemAcesso aberto (Open Access)Galactosaminoglycans from normal myometrium and leiomyoma(Associação Brasileira de Divulgação Científica, 2001-05-01) Berto, A.g.a. [UNIFESP]; Oba-Shinjo, Sueli Mieko [UNIFESP]; Michelacci, Yara Maria [UNIFESP]; Sampaio, L.o. [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)In many tumors, the amount of chondroitin sulfate in the extracellular matrix has been shown to be elevated when compared to the corresponding normal tissue. Nevertheless, the degree of chondroitin sulfate increase varies widely. In order to investigate a possible correlation between the amount of chondroitin sulfate and tumor size, several individual specimens of human leiomyoma, a benign uterine tumor, were analyzed. The glycosaminoglycans from eight tumors were extracted and compared with those from the respective adjacent normal myometrium. The main glycosaminoglycan found in normal myometrium was dermatan sulfate, with small amounts of chondroitin sulfate and heparan sulfate. In leiomyoma, both dermatan sulfate and chondroitin sulfate were detected and the total amounts of the two galactosaminoglycans was increased in all tumors when compared to normal tissue. In contrast, the heparan sulfate concentration decreased in the tumor. To assess the disaccharide composition of galactosaminoglycans, these compounds were incubated with bacterial chondroitinases AC and ABC. The amounts of L-iduronic acid-containing disaccharides remained constant, whereas the concentration of D-glucuronic acid-containing disaccharides increased from 2 to 10 times in the tumor, indicating that D-glucuronic acid-containing disaccharides are responsible for the elevation in galactosaminoglycan concentration. This increase is positively correlated with tumor size.
- ItemSomente MetadadadosGestational tissue inflammatory biomarkers at term labor: Asystematic review of literature(Wiley, 2018) Hadley, Emily E.; Richardson, Lauren S.; Torloni, Maria R. [UNIFESP]; Menon, RamkumarParturition at term is characterized by inflammatory overload in both feto-maternal tissues. Despite the large number of individual studies on changes in inflammatory biomarkers linked to labor, a comprehensive profile of them in each of the uterine compartments is not available to better understand their mechanistic contributions to labor. This systematic review investigated the pro- and anti-inflammatory biomarkers reported in intra-uterine tissues (amnion, chorion, decidua, placenta, and myometrium) at term labor. We conducted a systematic review of studies on pro- and anti-inflammatory biomarkers (mRNA and/or protein) reported in feto-maternal tissues during normal human term labor, published in English (1980-2016), in 3 electronic data bases. From a total of 3712 citations, 172 were included for final review. Each tissue expresses a unique set of biomarkers at the time of term labor, but there is significant overlap between tissues. All tissues had IL-6, IL-8, IL-1, COX-2, PGE-2, TNF-, and hCAP18 in common at term labor. Common and unique inflammatory biomarkers are expressed in various feto-maternal compartments at term labor. Increase in pro-inflammatory markers in all gestational tissue signifies their harmonious functional role in promoting labor. Anti-inflammatory markers at term labor are hardly reported.
- ItemSomente MetadadadosHistomorphometric analysis of the effects of creatine on rat myometrium(Informa Healthcare, 2012-08-01) Do Amaral, Vinicius Cestari; Simoes, Manuel de Jesus [UNIFESP]; Marcondes, Rodrigo Rodrigues; Matozinho Cubas, Jairo Jose [UNIFESP]; Baracat, Edmund Chada; Soares, Jose Maria [UNIFESP]; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP)Objective: To analyze the myometrial thickness of rats subjected to creatine (Cr) ingestion. Study design: A total of 14 rats was equally divided into the control group (ConGr) receiving 1 ml potable water and the creatine group (CrGr) subjected to the ingestion of 1.6 g/kg Cr diluted in 1 ml potable water. At the end of 8 weeks, the animals were anesthetized (xylazine and ketamine) and sacrificed, the uteri and ovaries stained with hematoxylin and eosin, the thickness of both the myometrium and the epithelium measured and the follicles counted. Results: Analysis revealed a significant increase in thickness of the myometrium in the CrGr (272.26 +/- 66.71 mu m) contrasted with that from the ConGr (160.76 +/- 35.65 mu m), CrGr > ConGr (p < 0001). Conclusion: Our data suggest that Cr changed myometrial morphology in rats by enhancing myometrial thickness, but its action mechanism in the smooth muscle is still unclear.
- ItemSomente MetadadadosMalignant peripheral nerve sheath tumor of the uterine corpus: A case report(Lippincott Williams & Wilkins, 2007-10-01) Focchi, Gustavo R. de A.; Cuatrecasas, Miriam; Prat, Jaime; Autonomous Univ Barcelona; Universidade Federal de São Paulo (UNIFESP)Uterine malignant peripheral nerve sheath tumors (MPNST) are rare and have been reported in the cervix. We describe a case of MPNST of the uterine corpus, and discuss its differential diagnosis.