Navegando por Palavras-chave "mola hidatiforme"

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    Mola hidatiforme - achados anatomopatológicos, imuno-histoquimicos e moleculares
    (Universidade Federal de São Paulo (UNIFESP), 2016-12-14) Martinez, Rosana Mercante [UNIFESP]; Alves, Maria Teresa de Seixas Alves [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Hydatidiform mole (HM) is a benign tumor of placental trophoblastic cells, secondary to a fertility disorder causing abnormal development of the placenta. It can be classified as complete hydatidiform mole (CM) and partial hydatidiform mole (PM). The MC is diploid and the karyotype is 46, XX or 46, XY. In both cases we have the formation of a diploid zygote with duplication of paternal genetic load (diandria). On the other hand, the MP is triploid and 70% of the cases present the karyotype is 69, XXY. The distinction between the two types of HM and HM and not molar specimens (MN) is very important in view of differential risk for the development of persistent gestational trophoblastic disease. Aim: To correlate the pathological findings, immunohistochemical and molecular and miscarriage 27 samples sent to the Department of Pathology - UNIFESP, assessing the importance of complementary methods (STR and Immunohistochemistry) in the diagnosis of molar abortion. Methods: Twenty-seven samples were selected from the Department of Pathology file. Immunohistochemical analysis was reviewed by the pathologist and some samples that had no prior immunohistochemistry were required to perform the technique. To assess ploidy DNA was extracted from the samples and used genotyping of microsatellite technique (STR). Results: Correlating the initial pathology findings, immunohistochemical and molecular, we observed that 77,8% of cases were concordant, 14,8% discordant and 7,4% inconclusive. All discordant diagnosis were MC and triploid. Some cases of PM X MN the ploidy studies didn´t aid in the differential diagnostic, so the DNA ploidy in two cases was triploid. Conclusion: IHC evaluation of p57 protein is a practical and suitable for distinguishing between CM and PM. The analysis of p57 can be used with the ploidy studies to refine the diagnosis of early molar pregnancies