Navegando por Palavras-chave "mdx mice"

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    Característica do processo de regeneração de músculos esqueléticos de diferentes origens embrionárias em camundongos mdx submetidos ao treinamento físico aeróbio
    (Universidade Federal de São Paulo (UNIFESP), 2016-03-18) Lazzarin, Mariana Cruz [UNIFESP]; De Oliveira, Flavia [UNIFESP]; http://lattes.cnpq.br/3387760393535776; http://lattes.cnpq.br/1579497511371132; Universidade Federal de São Paulo (UNIFESP)
    Duchenne muscular dystrophy (DMD) is a genetic disorder that generates loss of functionality, with impaired strength and muscle mass. The effect of exercise on muscle dystrophic is not well established. Thus, the aim of this study was to analyze the effects of aerobic exercise training on the morphology and myogenic markers of muscle regeneration and inflammation of skeletal muscles from different embryonic origins in C57BL/10-Dmdmdx mice. Were used male C57BL/10 mice (n = 10) and C57BL/10-Dmdmdx (n = 10), with eight weeks old. They were distributed into four groups (n = 5): Sedentary Control (SC), Trained Control (TC), Sedentary Mdx (S-Mdx) and Trained Mdx (T-Mdx). The trained groups were submitted to seven weeks of aerobic exercise, five times weekly at a high intensity speed for 60 minutes with treadmill downward of -15°. After euthanasia, the gastrocnemius muscles (somitic embryonic origin) and masseter (branchiomeric embryonic origin) were dissected and processed for undergoing: Histopathological; morphometric (cell density of transverse sections and the cross-sectional area of muscle fibers); immunohistochemistry markers for muscle regeneration (MyoD, and Myogenin), inflammation (COX-2) and oxidative stress (8-OHdG). Histopathological results showed morphological changes in Mdx groups as well as the quantification of connective tissue, both in the gastrocnemius muscle and masseter. The morphometric analysis revealed that Mdx groups (S-Mdx and T-Mdx) have heterogeneity in the muscle fibers and the gastrocnemius muscle when compared with masseter muscle, which had a similar pattern to the control groups (SC and TC). Immunohistochemical results showed less nuclear staining MyoD and Myogenin for Mdx groups (SMdx and T-Mdx) when compared with control groups (SC and TC) for gastrocnemius muscle. Masseter muscle, differed between Mdx and Control groups about Myogenin parameter. In both muscles, the Mdx-T group had higher percentage of immunoreactive nuclei Myogenin compared to Mdx-S. Immunohistochemical results of COX-2 were higher for Mdx groups in both muscles, but only the gastrocnemius muscle was influenced training. The immunoreactivity of 8-OHdG showed statistical significance only about physical training and in gastrocnemius muscle. The conclusion of the study showed that the embryonic origin of skeletal muscle influenced the response of muscular dystrophy and exercise. Physical training protocol modified the histopathology and the presence of connective tissue in the masseter muscle. In addition, physical training interfered in increased immunoreactivity of COX-2 in the gastrocnemius muscle dystrophic. Furthermore, it was able to increase Myogenin in both muscles and groups.