Navegando por Palavras-chave "mRNA expression"
Agora exibindo 1 - 4 de 4
Resultados por página
Opções de Ordenação
- ItemSomente MetadadadosA Daily Dose of 5 mg Folic Acid for 90 Days Is Associated with Increased Serum Unmetabolized Folic Acid and Reduced Natural Killer Cell Cytotoxicity in Healthy Brazilian Adults(Amer Soc Nutrition-Asn, 2017) Paniz, Clovis; Bertinato, Juliano Felix; Lucena, Maylla Rodrigues [UNIFESP]; De Carli, Eduardo; da Silva Amorim, Patricia Mendonca; Gomes, Guilherme Wataru; Palchetti, Cecilia Zanin; Figueiredo, Maria Stella [UNIFESP]; Pfeiffer, Christine M.; Fazili, Zia; Green, Ralph; Guerra-Shinohara, Elvira Maria [UNIFESP]Background: The effects of high-dose folic acid (FA) supplementation in healthy individuals on blood folate concentrations and immune response are unknown. Objective: The aim of the study was to evaluate the effects of daily consumption of a tablet containing 5 mg FA on serum folate
- ItemSomente MetadadadosDifferential expression of Ro/SSA 60 kDa and La/SSB, but not Ro/SSA 52 kDa, mRNA and protein in minor salivary glands from patients with primary Sjogren's syndrome(J Rheumatol Publ Co, 2007-06-01) Barcellos, Karin S. A. [UNIFESP]; Nonogaki, Suely [UNIFESP]; Enokihara, Milvia M. S. S. [UNIFESP]; Teixeira, Miriam S. [UNIFESP]; Andrade, Luis E. C. [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Adolpho Lutz InstObjective. To analyze the protein and messenger RNA (mRNA) expression of La/SSB, Ro/SSA 60, and Ro/SSA 52 antigens in minor salivary glands (MSG) from patients with primary Sjogren's syndrome (pSS).Methods. La/SSB, Ro/SSA 60, and Ro/SSA 52 protein expression was studied by immunohistochemistry in MSG from 26 patients with pSS and 16 controls. mRNA expression was determined by real-time polymerase chain reaction in MSG of 10 patients with pSS and 7 controls.Results. La/SSB and Ro/SSA 60, but not Ro/SSA 52, mRNA expression was higher in samples from patients with pSS compared to controls (p < 0.05). La/SSB protein had higher expression in the cytoplasm of ductal cells than in the cytoplasm of mucous acinar cells in patients with pSS (p = 0.013) but not in controls. Ro/SSA 60 had higher expression in the cytoplasm of ductal cells than in the cytoplasm of serous acinar cells in patients with pSS (p = 0.006) but not in controls. The Ro/SSA 52 expression pattern was similar in patients and controls. There was no association between circulating autoantibodies to Ro/SSA or La/SSB and the aberrant expression of the cognate autoantigens.Conclusion. The increased Ro/SSA 60 and La/SSB mRNA expression in MSG of patients with pSS as well as the differential Ro/SSA 60 and La/SSB protein expression in ductal cells of MSG in patients with pSS suggest that these these 2 autoantigens, but not Ro/SSA 52, are probably involved in triggering and maintaining the tissue-specific autoimmune response in pSS MSG and may contribute to the antigen-driven immune response and local autoantibody production
- ItemSomente MetadadadosExpression and Prognostic Relevance of GAGE1 and XAGE1 Cancer/Testis Antigens in Head and Neck Squamous Cell Carcinoma(Bentham Science Publ Ltd, 2017) Karia, B. T. R. [UNIFESP]; Zamuner, F. T. [UNIFESP]; Carlin, V. [UNIFESP]; de Oliveira, C. Z.; Carvalho, A. L.; Vettore, A. L. [UNIFESP]Background: In the last decades, survival rates in head and neck squamous cell carcinoma (HNSCC) have not changed, with a five-year survival of only 50%. Thus, there is a great need for the identification of new molecular targets and development of novel therapeutic strategies. Cancer-testis antigens (CTAs) are expressed in various types of tumor but rarely in healthy normal tissues. Therefore, they appear as ideal targets for immunotherapy approaches, as well as, unique markers for cancer diagnosis/prognosis. Objective: This study evaluated the expression pattern of cancer/testis antigens (CTA) in HNSCC samples and correlated the expression data with the clinicopathological prognostic variables. Methods: An in silico screening was performed using all CTA genes cataloged on the CTDatabase and the expression of the eight CTA genes (ARMC3, DDX53, FTHL17, GAGE1, MAGEA11, SYCE1, TCP11, and XAGE1) was examined in 89 HNSCC and 20 normal mucosa samples using RT-PCR analysis. Results: GAGE1 (48.3%), XAGE1 (40.4%) and MAGEA11 (19.1%) were frequently and specifically expressed in HNSCC samples and 68.5% of the cases expressed at least one of these antigens. Moreover, GAGE1 and XAGE1 mRNA positivity was significantly associated with the presence of metastasis in the lymph nodes (p=0.038 and p=0.023, respectively) and, by multivariate analysis, male gender (p=0.032), advanced clinical stage (p=0.018) and mRNA positivity for GAGE1 (p=0.010) were independent prognostic factors for overall survival. Conclusion: These findings suggest GAGE1 and XAGE1 expressions to be useful as prognostic markers for HNSCC.
- ItemSomente MetadadadosGenetic variants in gastric cancer: Risks and clinical implications(Academic Press Inc Elsevier Science, 2017) Gigek, Carolina Oliveira [UNIFESP]; Calcagno, Danielle Queiroz; Rasmussen, Lucas Trevizani; Santos, Leonardo Caires [UNIFESP]; Leal, Mariana Ferreira [UNIFESP]; Wisnieski, Fernanda [UNIFESP]; Burbano, Rommel Rodriguez; Lourenco, Laercio Gomes [UNIFESP]; Lopes-Filho, Gaspar Jesus [UNIFESP]; Cardoso Smith, Marilia Arruda [UNIFESP]Cancer is a multifactorial disease that involves many molecular alterations. Gastric cancer (GC) is the third leading cause of cancer death worldwide. GC is a highly heterogeneous disease with different molecular and genetics features. Therefore, this review focuses on an overview of the genetic aspects of gastric cancer by highlighting the important impact and role of deletions and/or duplications of chromosomal segments, genomic variants, H. pylori infection and interleukin variants, as found in gene expression and newly proposed molecular classification studies. The challenge is to better understand the mechanisms and different pathways that lead to the development and progression of GC.