Navegando por Palavras-chave "internalization and recycling of receptors"

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    Effects of carbachol on rat Sertoli cell proliferation and muscarinic acetylcholine receptors regulation: an in vitro study
    (Elsevier B.V., 2004-08-20) Lucas, TFG; Avellar, MCW; Porto, C. S.; Universidade Federal de São Paulo (UNIFESP)
    The aim of the present work was to study the effect of muscarinic agonist on cell proliferation and muscarinic acetylcholine receptors (mAChRs) regulation in rat Sertoli cells. Primary cultures of Sertoli cells were obtained from 8-day and, 15-day old male Wistar rats. in proliferation assays, [methyl-H-3]thymidine incorporation in Sertoli cells from 8-day and 15-day old rats reached a plateau after 60 min of carbachol incubation and decreased after 120 min of agonist incubation. Binding studies with [N-Methyl-H-3]scopolamine ([H-3]NMS) indicated a rapid loss of cell surface mAChRs when Sertoli cells from 15-day old rats were incubated with carbachol at 35 degreesC for 2 min. This effect was temperature-dependent. When the incubation of the cells was prolonged at 35 degreesC or at 4 degreesC, after the agonist had been washed away, 94% of mAChRs were present in the cell surface after 120 min incubation at 35 degreesC. At 4 degreesC, however, a low percentage of mAChRs was detected in the cell surface. in the presence of cycloheximide, the recycling of mAChRs to the cell surface was not changed, suggesting that the appearance of mAChRs on cell surface was not dependent on de novo receptor synthesis. in conclusion, our studies indicate that the activation of mAChRs may play a role in rat Sertoli cell proliferation. These receptors may be under regulation (internalization and recycling) when cells are exposed to muscarinic cholinergic agonist. (C) 2004 Elsevier Inc. All rights reserved.