Navegando por Palavras-chave "in vitro fertilization"
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- ItemAcesso aberto (Open Access)Avaliação da expressão gênica das vias de sinalização de insulina em células da granulosa provenientes de mulheres eutróficas e obesas com síndrome dos ovários policísticos em ciclos de fertilização in vitro(Universidade Federal de São Paulo (UNIFESP), 2015-11-27) Chehin, Mauricio Barbour [UNIFESP]; Motta, Eduardo Leme Alves da [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Objective: To evaluate granulosa cell gene expression from insulin signaling pathway of Polycystic Ovarian Syndrome (PCOS) patients after In Vitro Fertilization (IVF) and compare the granulosa gene expression activity between eutrophic and obese women. Methods: Cross-sectional study with 15 PCOS patients, nine eutrophic and six obese, which were submitted to oocyte recovery to IVF and after the procedure had the granulosa cumulus cells removed from the oocyte to RNA extraction and later quantitative PCR array analysis of expression gene profile. The results were expressed by fold up or fold down of obese patients gene expression over eutrophic patients gene expression, fold ? 3 or ? 3 values and p ? 0,05 were considered statiscally significant. Results: The analyzed genes are overexpressed in obese compared to eutrophic women. There are 09 genes, BCL2L1, BRAF, CBL, DOK1, FBP1, FRS2, PCK2, RPS6KA1 and SORBS1 that presents fold ? 3 and p ? 0,05. Conclusions: In the group OB significantly overexpressed genes are responsible for the proliferation and differentiation of cumulus cells during oocyte maturation, insulin resistance, regulation of apoptosis, and glucose metabolism during early embryogenesis.
- ItemSomente MetadadadosEndometrial leukemia inhibitory factor as a predictor of pregnancy after in vitro fertilization(Elsevier B.V., 2008-07-01) Serafini, Paulo; Rocha, Andre M.; Osorio, Cyntia T.; Silva, Ismael da [UNIFESP]; Motta, Eduardo L. [UNIFESP]; Baracat, Edmund C.; Huntington Reproduct Ctr; Universidade de São Paulo (USP); Hosp Canc AC Camargo; Universidade Federal de São Paulo (UNIFESP)Objective: To determine whether there is an association between endometrial expression of leukemia inhibitory factor (LIF) in the luteal phase of the menstrual cycle preceding in vitro fertilization (IVF) and treatment outcome. Methods: Biopsy specimens from the endometria of 52 women in the luteal. phase were immunostained against LIF Embryo culture and transfer were done according to standard procedures. Results: Clinical pregnancy occurred in 39% of the women following IVF, and strong endometrial immunohistochemical staining for LIF was associated with pregnancy (P=0.01). the women with a strong LIF expression had a 6.4-fold higher chance of becoming pregnant than those with weaker intensities (P=0.005). Conclusion: Endometrial expression of LIF during the luteal phase can be used as a predictor of IVF success. (C) 2008 International Federation of Gynecology and Obstetrics. Published by Elsevier Ireland Ltd. All rights reserved.
- ItemAcesso aberto (Open Access)How general semen quality influences the blastocyst formation rate: Analysis of 4205 IVF cycles(Soc Brasileira Reproducao Assistida-Sbra, 2018) Piccolomini, Mariana M.; Bonetti, Tatiana Carvalho de Souza [UNIFESP]; Motta, Eduardo Leme Alves da [UNIFESP]; Serafini, Paulo Cesar [UNIFESP]; Alegretti, Jose Roberto [UNIFESP]Objective: To select embryos with higher implantation potential, the extended culture has been the most frequently applied strategy worldwide, and consequently leads to higher live birth rates per transfer. Sperm quality is a determining feature, and it may influence the outcomes of IVF from fertilization to embryo development. Therefore, we hypothesize that blastocyst formation may also be impaired by general semen quality. Methods: We analyzed 4205 IVF cycles. Four study groups were designed according to semen quality: normal, mild alteration, severe alteration and epididymis. All cycles were intended to extend embryo culture until the blastocyst stage, and embryo development was evaluated. Results: Regarding cleavage rate, the normal and mild alteration semen groups were equivalent, and the severe alteration and epididymis semen groups were equivalent to each other. The blastocyst formation rate decreased with semen quality. At least one blastocyst formed in 79.9% of cycles for the normal semen group, whereas the percentage of cycles with the formation of at least one blastocyst was slightly lower for the mild alteration (75.6%), severe alteration (76.4%) and epididymis (76.8%) semen groups. A multivariate logistic regression showed that for each additional cleaved embryo on day 3, the chance of having at least one blastocyst doubles. Additionally, the chance of having at least one blastocyst decreased when semen presented mild or severe alterations. Conclusion: The general quality of sperm is a good predictor of blastocyst formation, significantly affecting the likelihood of having at least one blastocyst at the end of the cycle. Based on our findings, it is necessary to consider general semen quality and the number of cleaved embryos when forecasting the possibility of blastocyst formation and transfer in an extended culture system.
- ItemSomente MetadadadosOvarian stimulation with daily late follicular phase administration of low-dose human chorionic gonadotropin for in vitro fertilization: a prospective, randomized trial(Elsevier B.V., 2006-10-01) Serafini, Paulo; Yadid, Isaac; Motta, Eduardo L. A. [UNIFESP]; Alegretti, Jose R.; Fioravanti, Joyce; Coslovsky, Marcio; Huntington Ctr Reprod Med; Ctr Human Reprod Hosp & Matern Santa Joana; Universidade Federal de São Paulo (UNIFESP)Objective: Evaluate the effectiveness of a new ovarian stimulation (OS) protocol before IVF.Design: Prospective centers.Setting: Private centers.Patient(s): Three hundred and twenty-three intended-to-treat women candidates for IVF.Intervention(s): Patients were divided into three groups and administered the following treatments: group A, recombinant hFSH from day 3 until follicles reached 13-14mm, when recombinant hFSH was lowered to 75 IU daily and daily injections of 200 IU of hCG and a GnRH antagonist were administered until final maturation; group B, recombinant hFSH and a GnRH antagonist; group C, recombinant hFSH and a GnRH agonist.Main Outcome Measure(s): Primary outcome was the number of mature oocytes. Secondary outcomes included average initial and total recombinant hFSH dosage, serum E-2 level on day of ovulation, number of oocytes retrieved, fertilization, number of top-quality embryos, endometrial thickness, implantation rate, pregnancy rate (PR), and incidence of ovarian hyperstimulation syndrome (OHSS).Result(s): the numbers of oocytes retrieved, mature oocytes, fertilization, top-quality embryos, and embryos transferred were comparable in all groups. Implantation rate, PR, and incidence of OHSS were also comparable. the total dose of recombinant hFSH was significantly lower in group A (1,674.7 +/- 59.4 IU. vs. 2,197.9 +/- 77.8 IU in group B and 2,156.7 +/- 80.9 IU in group C).Conclusion(s): This new OS protocol permits follicles and oocytes to fully develop, helps generate top-quality embryos, avoids premature ovulation, establishes clinical pregnancies, reduces administration of recombinant hFSH, minimizes costs, and does not increase the chances of OHSS.
- ItemSomente MetadadadosPesquisa de biomarcadores proteicos para o sucesso da fertilização in vitro clássica através da análise proteômica do meio de cultura(Universidade Federal de São Paulo (UNIFESP), 2016-06-12) Moreira, Debora Carvalho [UNIFESP]; Spaine, Deborah Montagnini Spaine [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Introduction: Infertility is an important issue that affects many couples around the world and assisted reproduction technique more used in the treatment of these couples is the In Vitro Fertilization (IVF). The main challenge of IVF is to select an embryo with high potential implantation successful without generating unwanted multiple pregnancy. However, this selection is made subjectively by morphological analysis. New non-invasive methods are necessary for selection of embryos with higher implantation potential. Objectives: Evaluate the profile of secreted proteins during sperm-oocyte interaction in the conventional IVF process. Methods: We conducted a prospective cohort study including a total of 20 cases of couples that came to the Human Reproduction Division of UNIFESP and were suitable for conventional IVF. The patients underwent controlled ovarian stimulation as medical guidelines, followed by collection of ovarian follicles by transvaginal puncturing. Oocytes were placed in contact with spermatozoa previously selected in culture media for 18 hours.The remaining culture medium were pooledin the following groups according to the results of fertilization of oocytes and embryo development: normal fertilization (FN): presence of two pronuclei; fertilization failure (FF): absence of pronuclei; abnormal fertilization (FA): absence of pronuclei at the time of check fertilization, but there were embryonic development. The FN group was split into homogeneous development (F1) and heterogeneous (F3 and F4). The excess of albumin was removed from the sample, followed by fractionation and concentration using filter. The proteins were quantified, digested and analyzed by tandem mass spectrometry. Results: 73 proteins were identified. It was possible to identify proteins associated with successful fertilization and development. The main proteins marker were Perlecan (HSPG2) Anti-Thrombin-III (ANT 3), Apolipoprotein A-IV (APO4), Fibrinogenalpha Chain and Protein 4 Retinol-Binding (RET4) that were increased in the culture media and associated with success of IVF and homogeneous development. Conclusion: The embryos with fertilization success secreted a different protein profile from the cases of failure, and it was possible, from these proteins, to infer about embryo developmental outcome. These proteins may be potentially used in IVF success biomarkers in non-invasive testing of embryonic viability.