Navegando por Palavras-chave "immunomodulation"

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    Adjuvant effect of the propionibacterium acnes and its purified soluble polysaccharide on the immunization with plasmidial DNA containing a Trypanosoma cruzi gene
    (Center Academic Publ Japan, 2006-01-01) Mussalem, Juliana Sekeres [UNIFESP]; Vasconcelos, Jose Ronnie Carvalho de [UNIFESP]; Squaiella, Carla Cristina [UNIFESP]; Ananias, Renata Zeigler [UNIFESP]; Braga, Eleni Goncalves [UNIFESP]; Rodrigues, Mauricio Martins [UNIFESP]; Longo-Maugeri, Ieda Maria [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    In the present work we investigated the role of killed Propionibacterium acnes or a soluble polysaccharide extracted from bacterium cell wall in modulated experimental immunization with plasmidial DNA. We used a plasmid, p154/13, containing a gene-encoding catalytic domain of Trypanosoma cruzi (T cruzi) trans-sialidase. As previously described, immunization of BALB/c mice with p154/13 elicited Immoral, cell-mediated and protective immune responses against T cruzi infection. In this study we describe that both P acnes and its soluble polysaccharide fraction have the ability to modulate the immune response elicited by p154/13. Treatment with these adjuvants enhanced specific trans-sialidase Th1 immune response, as revealed by a lower IgG1/IgG2a ratio and stronger in vitro IFN-gamma synthesis by CD4(+) T cells. The most important fact was that treatment with P acnes or its soluble polysaccharide fraction in the presence of p154/13 significantly reduced the peak of parasitemia observed 7 to 8 days after T cruzi challenge. These data suggest that P acnes or its soluble polysaccharide fraction may improve the protective potential of a DNA vaccine against experimental T cruzi infection.
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    Effect of glucan on murine lupus evolution and on host resistance to Klebsiella pneumoniae
    (Wiley-Blackwell, 1997-01-01) Harima, Helena Aiko [UNIFESP]; Mendes, Nelson Figueiredo [UNIFESP]; Mamizuka, Elsa Masae [UNIFESP]; Mariano, Mario [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP)
    Glucan is a polysaccharide from the yeast Saccharomyces cerevisiae that stimulates the mononuclear phagocytic system (MPS). NZB/NZW F1 mice were divided into two groups: one group received a subcutaneous injection of 0.5 mg glucan/animal for 1 week, and the other received the same dose for 3 months. No changes were observed in those animals submitted to short-term glucan treatment, whereas animals with active lupus and submitted to long-term glucan administration presented early death, with significant differences in accumulated mortality rates over 33-37 weeks, when compared to controls. No deaths were observed in lupus mice treated with glucan 24 hours before the induction of septic shock by Klebsiella pneumoniae, in contrast to mortality of 95.3% in the control group during the follow-up period of 12 days. We conclude that although glucan is able to exacerbate lupus activity, it enhances resistance to infection in lupus mice. (C) 1997 Wiley-Liss, Inc.
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    In vivo and in vitro effect of killed Propionibacterium acnes and its purified soluble polysaccharide on mouse bone marrow stem cells and dendritic cell differentiation
    (Elsevier B.V., 2006-01-01) Squaiella, C. C.; Ananias, R. Z.; Mussalem, J. S.; Braga, E. G.; Rodrigues, E. G.; Travassos, L. R.; Lopes, J. D.; Longo-Maugeri, L. M.; Universidade Federal de São Paulo (UNIFESP)
    Among the effects exerted by Propionibacterium acnes, a most relevant one is its capacity to modulate the Th1/Th2 cellular immune response. This effect depends on the induction and activation of antigen presenting cells, mainly dendritic cells (DCs), whose number is increased in the peripheral blood of animals treated with this bacterium. A soluble P. acnes polysaccharide (PS) extract also acts on DCs, modulating a Th1 immune response. These data led us to investigate the role of P. acnes and its soluble PS on murine bone marrow (BM) DCs. Bone marrow cells were analyzed by flow cytometry, showing an increase of stem cells and DCs in P. acnes- or PS-treated animals. Culturing in the presence of granulocyte monocyte colony stimulating factor (GM-CSF) increased the in vitro differentiation and maturation of these cells into BM-derived DCs (CD11c(+) and MHC class II+). Maturation of DCs was determined by increased CD80 and CD86 expression, IL-4 and IL-12 production, reduction in phagocytic capacity and increase in the antigen presenting ability to primed or naive T lymphocytes. These data indicate that P. acnes as well as its PS can modulate BM stem cells, originating mature DCs, which are important mainly at the initial antigen contact. (c) 2005 Elsevier GmbH. All rights reserved.
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    Propionibacterium acnes Enhances the Immunogenicity of HIVBr18 Human Immunodeficiency Virus-1 Vaccine
    (Frontiers Media Sa, 2018) Teixeira, Daniela [UNIFESP]; Ishimura, Mayari Eika [UNIFESP]; Apostolico, Juliana de Souza [UNIFESP]; Viel, Jacqueline Miyuki [UNIFESP]; Passarelli, Victor Cabelho [UNIFESP]; Cunha-Neto, Edecio; Rosa, Daniela Santoro [UNIFESP]; Longo-Maugeri, Ieda Maria [UNIFESP]
    Immunization of BALB/c mice with HIVBr18, a DNA vaccine containing 18 CD4(+) T cell epitopes from human immunodeficiency virus (HIV), induced specific CD4(+) and CD8(+) T cell responses in a broad, polyfunctional and persistent manner. With the aim of increasing the immunogenicity of this vaccine, the effect of Propionibacterium acnes as an adjuvant was evaluated. The adjuvant effects of this bacterium have been extensively demonstrated in both experimental and clinical settings. Herein, administration of two doses of HIVBr18, in the presence of P. acnes, increased the proliferation of HIV-1-specific CD4(+) and CD8(+) T lymphocytes, the polyfunctional profile of CD4(+) T cells, the production of IFN-gamma, and the number of recognized vaccine-encoded peptides. One of the bacterial components responsible for most of the adjuvant effects observed was a soluble polysaccharide extracted from the P. acnes cell wall. Furthermore, within 10 weeks after immunization, the proliferation of specific T cells and production of IFN-gamma were maintained when the whole bacterium was administered, demonstrating a greater effect on the longevity of the immune response by P. acnes. Even with fewer immunization doses, P. acnes was found to be a potent adjuvant capable of potentiating the effects of the HIVBr18 vaccine. Therefore, P. acnes may be a potential adjuvant to aid this vaccine in inducing immunity or for therapeutic use.
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    Regulatory CD4(+) T lymphocytes after allogeneic transfusion in orthopaedic surgical patients
    (Wiley-Blackwell, 2017) Pereira, Luciana de Andrade [UNIFESP]; Baiocchi, Otavio Carvalho Guimarães [UNIFESP]; Silva, Joyce Matie Kinoshita da [UNIFESP]; Takata, Edmilson Takehiro [UNIFESP]; Bordin, Jose Orlando [UNIFESP]
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    T cell subpopulations in myocardial inflammatory infiltrates detected by confocal microscopy: dose dependence in mice treated with cyclophosphamide during acute Trypanosoma cruzi infection
    (Elsevier B.V., 2003-04-01) Calabrese, K. S.; Paradela, ASRC; Valle, T. Z. do; Tedesco, R. C.; Leonardo, R.; Mortara, R. A.; Costa, SCG da; Inst Oswaldo Cruz; Universidade Federal de São Paulo (UNIFESP)
    In this article, we have characterized cell subpopulations found in the hearts of mice presenting acute Chagas' disease by immunocytochemistry and subjected to different schedules of an immunosuppressive therapy with cyclophosphamide (CY). in this comparative study, CY treatment with different doses was carried out before or after infection with Trypanosoma cruzi Y strain trypomastigotes, enabling us to discriminate the parasitemic kinetics and inflammatory processes in the heart, 12 d after infection. Animals treated with 200 mg/kg of CY 2 d before infection presented high parasitaemia as well as heavy inflammation and low parasite loads in the heart. Mice treated 5 d after infection with the same dose, developed the same parasitaemic peak but were not able to control it. Their heart did not present inflammation, but a high number of parasites could be seen. Animals treated with five 3 mg/kg doses of CY every other day presented heavy inflammatory reaction and low parasitaemia. in this group, as well as the one treated before infection, immunocytochemistry studies have shown predominance of CD8(+) T cells in the myocardium. On the other hand, mice treated with 200 mg/kg of CY 5 d after infection, presented small amounts of CD4(+) T cells while no CD8(+) could be found. These results have confirmed the dose dependence influence of this drug on the T cell populations in the inflammatory infiltrates as well as the importance of the schedule employed. (C) 2003 Editions scientifiques et medicales Elsevier SAS. All rights reserved.
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    Treatment with Propionibacterium acnes modulates the late phase reaction of immediate hypersensitivity in mice
    (Elsevier B.V., 2003-08-05) Braga, E. G.; Ananias, R. Z.; Mussalem, J. S.; Squaiella, C. C.; Longhini, ALF; Mariano, M.; Travassos, L. R.; Longo-Maugeri, I. M.; Universidade Federal de São Paulo (UNIFESP)
    The administration of killed Propionibacterium acnes suspension to mice enhances macrophage phagocytic and tumoricidal activities, have an adjuvant effect to antibody response and increases resistance to infection. Recent reports demonstrated that P. acnes treatment promotes IL-12 and H-18 synthesis in mice inducing IFN-gamma release, enhancement of IgG2a switch and inhibition of Th2 cell expansion. These findings led us to investigate whether P. acnes could modulate hypersensitivity type I reaction observed in a murine model. Animals were implanted with heat coagulated hen's egg white (HEW) into the subcutaneous tissue, followed by OVA-challenge in the footpad. the observed reaction was characterized by elevated Th2 cytokine levels, especially IL-4 and increase in eosinophil infiltration as occurs in the late phase reaction (LPR) of type I hypersensitivity, a pattern observed in allergic asthma in human. Two different biological effects were induced by killed P. acnes depending on the experimental protocol used. When mice were treated with one dose of P. acnes per week during 3 weeks and the last dose administrated at the same time of HEW implantation, a strong adjuvant effect on type I hypersensitivity reaction with intense eosinophilic infiltration was observed. On the other hand, when the HEW implant was made 1 week after the administration of the last dose of P. acnes, animals developed a typical delayed type hypersensitivity reaction, and a cytokines pattern characteristic of the Th1 immune response. (C) 2003 Elsevier Science B.V. All rights reserved.