Navegando por Palavras-chave "genotoxicidade"

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    Avaliação do impacto da co-exposição do Triclosan e do Ftalato dehp sobre a viabilidade celular e a estabilidade genética, in vitro
    (Universidade Federal de São Paulo (UNIFESP), 2020-01-15) Duarte, Nathalia De Assis Aguilar [UNIFESP]; Barcelos, Gustavo Rafael Mazzaron [UNIFESP]; Universidade Federal de São Paulo
    Triclosan (TCS) is an antimicrobial agent widely used in Personal Care Products (PCPs) and Di-(2-ethylhydroxyl-phtalate) (DEHP) is a phthalate, a group of chemical compounds derived from phthalic acid, used in medical devices and plastic products with Polyvinyl Chloride (PVCs). As a result of extensive use, TCS and DEHP has been found in the environment and previous studies have demonstrated the interaction between their exposure and genotoxic damage to aquatic organisms. However, there is a shortage in the literature of these effects on human cells. Therefore, the aim of this study is to evaluate the cytotoxicity, genotoxicity, mutagenicity and interaction between TCS and DEPH compounds in HepG2 human hepatocarcinoma cell culture. Three different concentrations of each drug were chosen using the MTT test (0,10 μM, 1 μM e 10 μM) and 8 different combinations were evaluated later (e.g. TCS 10 μM + DEHP 1μM). The number of micronuclei (MN), nucleoplasmic bridges (NPBs), nuclear buds (NBUDs), nuclear division index (NDI), nuclear division cytotoxic index (NDCI), apoptotic and necrotic cells were evaluated. The results have shown that the three concentrations of TCS and the two highest DEHP concentrations increased the number of MN (p<0,05) as well as their combinations. The lower concentrations of both drugs had lower NDI and NDCI (p<0,05) when compared to the control group (DMSO 1%). The compounds showed synergistic and antagonist effects in several parameters analyzed. Finally, the results have shown a significant mutagenic (increase of MN) and cytostatic (decrease of NDI) and cytotoxic (decrease of NDCI) damage especially with co-exposure treatments, which is a more realistic model than studying drugs separately.