Navegando por Palavras-chave "genistein"

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    Collagen concentration on the facial skin of postmenopausal women after topical treatment with estradiol and genistein: a randomized double-blind controlled trial
    (Taylor & Francis Ltd, 2017) Silva, Lidia Aragao; Ferraz Carbonel, Adriana Aparecida [UNIFESP]; Barbosa de Moraes, Andrea Regina [UNIFESP]; Simoes, Ricardo S.; da Silva Sasso, Gisela Rodrigues [UNIFESP]; Goes, Livia [UNIFESP]; Nunes, Winnie [UNIFESP]; Simoes, Manuel Jesus{UNIFESP]; Patriarca, Marisa Teresinha [UNIFESP]
    Objective: The objective of this study is to compare the effects of topical estrogen and genistein (a soy isoflavone) on the facial skin collagen of postmenopausal women not undergoing systemic hormonal therapy.Methods: This is a prospective, double blind, randomized, controlled clinical trial. Volunteer women (N=30) 45-55year old from the Endocrine Gynecology sector of the Gynecology Department of the Federal University of SAo Paulo (UNIFESP). The Ethical Committee of the Federal University of SAo Paulo approved the study (report no. 386/2004
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    Concentração de colágeno na pele facial de mulheres na pós-menopausa após tratamento com estradiol e genisteína tópicos: um estudo randomizado duplo cego
    (Universidade Federal de São Paulo (UNIFESP), 2016-07-29) Sampaio, Lidia Lima Aragao [UNIFESP]; Patriarca, Marisa Teresinha [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Objective: To compare the known effects of estrogen with genistein on the facial skin collagen of postmenopausal women without hormone therapy. Methods: This is a clinical, cross over trial with randomized and blinded convenience sample with estrogen and isoflavones, which 30 volunteer postmenopausal women were recruited to quantify facial collagen concentration before and after topical use of estrogen and isoflavones for 24 weeks. Results: The amount of both types I and III collagen increased at the end of both treatments, but estrogen was superior to the phytoestrogen, with statistical significance. Conclusion: Treatment with topical estrogen is superior to phytoestrogen, but both showed an increase in facial skin collagen, with statistical significance. What is not yet clear is whether prolonged topical use of such substances can cause systemic effects, thus, more research is needed to clarify this question.
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    Expressão gênica dos receptores de estrogênios, das ciclinas e dos marcadores de proliferação celular pela genisteína no útero de ratas tratadas imediatamente ou 30 dias após ooforectomia
    (Universidade Federal de São Paulo (UNIFESP), 2013-09-25) Carbonel, Adriana Aparecida Ferraz [UNIFESP]; Simoes, Manuel de Jesus Simoes [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Objective: Identification of potential genes of the signaling cascade of estrogen receptors expressed in the uterus of rats treated immediately or lately with genistein or 17β-estradiol. Methods: 60 rats (Rattus norvegicus albinus) females, adult, ± 3 months old were divided into six (6) groups: GI immediate control - animals received vehicle; GII late control - animals received vehicle after 30 days of ovariectomy; GIII immediate genistein - animals treated with genistein (50mg/Kg) 30 days after ovariectomy; GIV late genistein - animals treated with genistein (50mg/Kg) 30 days after ovariectomy; GV immediate estrogen - animals treated with estrogen (17β-estradiol, 10μg/Kg) after ovariectomy and GVI late estrogen - animals treated with estrogen (17β-estradiol, 10μg/Kg) 30 days after ovariectomy. All animals were treated for 30 consecutive days. At the end of treatment, they were anesthetized and part of the uteri removed and immediately immersed in liquid nitrogen for molecular biological analysis and another part in 10% formaldehyde for subsequent histological processing in paraffin for morphological and immunohistochemical study. Statistical analyzes were performed using the software GraphPadPrism ® 5.0 (GraphPad Software Inc.). Statistical test used was one-way ANOVA followed by Tukey test (p < 0,05). Results: Relative expression of ESR2 genes , VEGF - A, MKI67, CCNA1, CCNA2, CCND1, CDK1A, CDKN1B, CDKN1A, CDKNB1 was generally very similar in animals treated immediately or later with genistein (GVI > GV > GIV = GIII > GII = GI), and immediate treatment in the ESR1 gene, showed a higher expression in GIII, however, when compared with GIV was lower than groups GV and GVI . Morphological data showed similar morphology and morphometry in immediate or late treatment. Thus, showing higher endometrial thickness, endometrial surface epithelium, number of glands, glandular circular area, myometrium area and the number of vessels in groups GIV and GV, when compared to other groups. Likewise we observed similar immunohistochemistry results from the beginning of treatment period. As scores of Vegf and Ki67 were higher in GVI (GVI > GV > GIV = GIII > GII = GI). Conclusion: Our data showed that treatment with genistein immediately or 30 days after ovariectomy were similar in relation to the genes of the estrogen receptor pathway, but the immediate treatment have observed increased expression of alpha receptors. The treatment with estrogens obtained greater activation of the gene via the estrogen receptors when administered lately.