Navegando por Palavras-chave "embryo implantation"
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- ItemSomente MetadadadosEffects of melatonin on the endometrial morphology and embryo implantation in rats(Elsevier B.V., 2008-05-01) Dair, Elisabete Lilian [UNIFESP]; Simoes, Ricardo Santos [UNIFESP]; Simoes, Manuel Jesus [UNIFESP]; Romeu, Lucrecia Regina Gomes [UNIFESP]; Oliveira-Filho, Ricardo Martins; Haidar, Mauro Abi [UNIFESP]; Baracat, Edmund Chada [UNIFESP]; Soares, Jose Maria [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP)Objective: To determine the effects of melatonin on rat endometrium morphology and embryo implantation.Design: Experimental study.Setting: Federal University of São Paulo, Brazil.Animal(s): Forty female rats.Intervention(s): GI: control, GII: sham-operated, GIII: pinealectomized, and GIV: pinealectomized rats that received melatonin during 3 months. the GI, GII, and Gin groups received the vehicle of melatonin (NaCl + ethanol). At the end of the treatment, the animals were killed during the estrous phase; the uterus was removed for morphometric analysis. Urine was collected for 6-sulfatoxymelatonin. Blood was collected for estrogen (E) and progesterone (P) level determinations. in a second experiment, female rats were used to evaluate the endometrial embryo implantation.Main Outcome Measure(s): Endometrial morphology and embryo implantation.Result(s): Gin presented the highest values for endometrial area and thickness index, number of endometrial glands, and eosinophils. the number of vessels of groups I, II, and IV was fewer than that of Gin. the highest number of eosinophils was detected in Gin in comparison to other groups. the implantation rate in Gin was the lowest of all groups. This implantation rate was significantly increased and restored toward normal in GIV.Conclusion(s): Our data suggested that, in nonphotoperiodic animals such as rats, melatonin may positively affect the endometrial morphology and improve embryo implantation.
- ItemAcesso aberto (Open Access)Endométrio na janela de implantação em mulheres com síndrome dos ovários policísticos(Associação Médica Brasileira, 2011-12-01) Lopes, Ione Maria Ribeiro Soares [UNIFESP]; Baracat, Maria Cândida Pinheiro; Simões, Manuel de Jesus [UNIFESP]; Simões, Ricardo Santos; Baracat, Edmund Chada [UNIFESP]; Soares Júnior, José Maria [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade Federal do Piauí; Universidade de São Paulo (USP)The human endometrium undergoes to a complex series of prolifertive and secretory changes in each menstrual cycle and displays only a short period of receptivity, known as the window of implantation, necessary for the implantation of the blastocyst in the uterus. The implantation process occurs in a sequential manner, leading to the establishment of pregnancy. Morphofunctional changes during this period may prevent or hinder the implantation. For this reason, the study of the endometrium at this stage is important for the improvement of therapies that may interfere with the mechanisms involved in maternal-embryonic interaction. Several gynecological disorders, including polycystic ovary syndrome (PCOS), are associated with decreased fertility and uterine receptivity. In spite of recent advances in assisted reproduction techniques, allowing the selection of high quality embryos, the implantation rate remains low and has not increased enough in recent decades. This article aims at reviewing the endometrial aspects of the window of implantation in women with polycystic ovary syndrome, focusing mainly on adhesion molecules. For that purpose, we analyzed 105 articles published in journals indexed in PubMed in the last 50 years (up to May 2011). In conclusion, the endometrial receptivity seems to be the major limiting factor for the establishment of pregnancy in a large number of gynecological diseases, including PCOS, and treatment to improve implantation rates is likely to be taken towards this direction.
- ItemSomente MetadadadosMetastatic melanoma positively influences pregnancy outcome in a mouse model: could a deadly tumor support embryo life?(Springer, 2008-03-01) Bollos, Rubens Harb [UNIFESP]; Nakamura, Mary Uchiyama [UNIFESP]; Valero-Lapchick, Valderez Bastos [UNIFESP]; Bevilacqua, Estela Maris Andrade Forell; Correa, Mariangela [UNIFESP]; Daher, Silvia [UNIFESP]; Ishigai, Marcia Marcelino de Souza [UNIFESP]; Jasiulionis, Miriam Galvonas [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP)The incidence of melanoma is increasing worldwide. It is one of the leading cancers in pregnancy and the most common malignancy to metastasize to placenta and fetus. There are no publications about experimental models of melanoma and pregnancy. We propose a new experimental murine model to study the effects of melanoma on pregnancy and its metastatic process. We tested several doses of melanoma cells until we arrived at the optimal dose, which produced tumor growth and allowed animal survival to the end of pregnancy. Two control groups were used: control (C) and stress control (SC) and three different routes of inoculation: intravenous (IV), intraperitoneal (IP) and subcutaneous (SC). All the fetuses and placentas were examined macroscopically and microscopically. the results suggest that melanoma is a risk factor for intrauterine growth restriction but does not affect placental weight. When inoculated by the SC route, the tumor grew only in the site of implantation. the IP route produced peritoneal tumoral growth and also ovarian and uterine metastases in 60% of the cases. the IV route produced pulmonary tumors. No placental or fetal metastases were obtained, regardless of the inoculation route. the injection of melanoma cells by any route did not increase the rate of fetal resorptions. Surprisingly, animals in the IV groups had no resorptions and a significantly higher number of fetuses. This finding may indicate that tumoral factors released in the host organism to favor tumor survival may also have a pro-gestational action and consequently improve the reproductive performance of these animals.
- ItemSomente MetadadadosMetoclopramide-induced hyperprolactinaemia caused marked decline in pinopodes and pregnancy rates in mice(Oxford Univ Press, 2006-10-01) Panzan, Michele Quarante [UNIFESP]; Soares Junior, José Maria [UNIFESP]; Motta, Eduardo Leme Alves da [UNIFESP]; Haapalainen, Edna Freymuller [UNIFESP]; Jesus Simoes, Manuel de [UNIFESP]; Baptista, Heloisa Allegro [UNIFESP]; Haidar, Mauro Abi [UNIFESP]; Baracat, Edmund Chada [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)BACKGROUND: the impact of hyperprolactinaemia on endometrial function, along with embryo implantation, has been the subject of discussion. This article examines whether experimental hyperprolactinaemia can affect mouse ovarian function, endometrial pinopodes and embryo implantation. METHODS: for pinopode analysis, 60 female mice were randomly divided into two groups: control (vehicle) and experimental [metoclopramide (MCP) 200 mu g per day]. Injections were given subcutaneously for 50 days, and then, normally cycling females were housed with male mice for copulation during proestrus. the animals were killed on the fifth day following coitus when the antimesometrium portions of the uterine horns were removed for endometrial analysis. Blood was collected for prolactin (PRL) determination. in the second experiment, 60 female mice were used to evaluate the ovarian function by measuring estrogen and progesterone levels and counting luteal bodies and oocytes in the oviduct and uterus during estrus. RESULTS: the highest pregnancy rates and the largest population of pinopodes were both found in the vehicle group (P < 0.01). Estrogen and progesterone levels in MCP-treated mice were lower than those in control mice (P < 0.05). Also, the number of implantations was significantly lower in the MCP-treated group compared with the vehicle group after embryo transfer (P < 0.001). CONCLUSION: PRL seems to have suppressive effects on ovarian function and the number of pinopodes; conceivably, hyperprolactinaemia has a negative effect on mouse embryo implantation.
- ItemAcesso aberto (Open Access)The predictive value of serum concentrations of anti-Mullerian hormone for oocyte quality, fertilization, and implantation(Soc Brasileira Reproducao Assistida-Sbra, 2017) Borges, Edson; Braga, Daniela P. A. F. [UNIFESP]; Setti, Amanda; Figueira, Rita de Cassia; Iaconelli, Assumpto, Jr.Objective: This study aimed to identify a possible correlation between serum levels of anti-.Mullerian hormone (AMH) and oocyte quality, embryo developmental competence, and implantation potential. Methods: 4488 oocytes obtained from 408 patients undergoing ICSI cycles were evaluated. Oocyte dimorphisms, embryo quality on days two and three, blastocyst formation competence, fertilization rates, implantation rates, and pregnancy rates were correlated with serum levels of AMH using Pearson's correlation coefficient and regression analysis. Results: A positive correlation was observed between serum levels of AMH and number of retrieved oocytes (CC: 0.600, p<0.001), fertilization rate (CC: 0.595, p=0.048), and number of obtained embryos (CC: 0.495, p<0.001). AMH did not affect the quality of cleavage stage embryos or the chance of blastocyst formation. However, AMH levels affected oocyte quality (OR: 0.75, CI 0.44-.0.96, p<0.001), and implantation (CC: 0,116, p=0.031) and pregnancy (OR: 1.22, CI: 1.03-.1.53, p<0.001) rates. Conclusion: Serum levels of AMH are a useful predictor of ovarian response to COS, oocyte quality, and fertilization. However, AMH levels may also compromise clinical outcomes