Navegando por Palavras-chave "Vascular endothelial growth factor-A"

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    Prognostic significance of vascular endothelial growth factor polymorphisms in colorectal cancer patients
    (Baishideng Publishing Group Inc, 2017) do Espirito Santo, Gilmar Ferreira [UNIFESP]; Galera, Bianca Borsatto; Duarte, Elisabeth Carmen; Chen, Elisabeth Suchi [UNIFESP]; Azis, Lenuce; Damazo, Amilcar Sabino; Saba, Gabriela Tognini [UNIFESP]; Gehrke, Flavia de Sousa; Cotrim Guerreiro da Silva, Ismael Dale [UNIFESP]; Waisberg, Jaques [UNIFESP]
    AIM To investigate the associations of the genetic polymorphisms of vascular endothelial growth factor A (VEGF-A) -1498C> T and -634G> C, with the survival of patients with colorectal cancer (CRC). METHODS A prospective cohort consisting of 131 Brazilians patients consecutively operated on with a curative intention as a result of sporadic colorectal carcinoma was studied. DNA was extracted from peripheral blood and its amplification and allelic discrimination for each genetic polymorphism was performed using the technique of polymerase chain reaction (PCR) in real-time. The real-time PCR technique was used to identify the VEGF-A -1498C> T (rs833031) and -634G> C (rs2010963) polymorphisms. Genotyping was validated for VEGF-A -1498C> T polymorphism in 129 patients and for VEGF-A -634G> C polymorphism in 118 patients. The analysis of association between categorical variables was performed using logistic regression, survival by Kaplan-Meier method and multivariate analysis by the Cox regression method. RESULTS In the univariate analysis there was a significant association (OR = 0.32