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- ItemAcesso aberto (Open Access)Suplementação materna de juçara (Euterpe Edulis Mart): na modulação dos marcadores inflamatórios e da microbiota da prole aos 21 dias de vida(Universidade Federal de São Paulo (UNIFESP), 2015-07-07) Morais, Carina Almeida [UNIFESP]; Pisani, Luciana Pellegrini [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Background. Maternal intake of TFAs in the perinatal period triggers a proinflammatory state and adiposity in offspring. However, phenol compounds contained in fruits are promising modulators of inflammation. Aim. This study investigated the effect of Jussara supplementation in the maternal diet on the development, microbiota and proinflammatory state of the colon in offspring exposed to perinatal TFAs. Methods. On the first day of pregnancy rats were divided into four groups: control diet (C), control diet with 0.5% Jussara supplementation (CJ), diet enriched with hydrogenated vegetable fat, rich in TFAs (T), or T diet supplemented with 0.5% Jussara (TJ) during pregnancy and lactation. Dams’ diets were maintained during pregnancy and lactation. After birth, litter sizes were adjusted to eight pups that remained with the mother. After 21 days, the offspring were decapitated. Trunk blood was collected and centrifuged. The RET, liver, colon and the fecal content were collected. Colon, liver and RET were used for quantification of mRNA relative levels by real-time PCR or perform Western Blotting. The genomic bacterial DNA in gut microbiota was performed by real-time PCR. The carcasses were eviscerated and used to determine lipid and protein content. Results. Maternal intake of TFAs resulted in increased IL-6 in RET and colon, TNF-�� e mRNA TLR-4 no cólon, MyD88 in the liver and a reduction in IL-10/TNF-α ratio and Bifidobacterium spp. in the offspring’s colon. However, Jussara supplementation restored the fecal content of Lactobacillus spp. e Bifidobacterium spp. (TJ vs T), increased colonic ZO-1 mRNA expression (TJ vs T), and decreased MyD88, p-NFkB p65 subunit (TJ e CJ vs T) and TNF-��R1 (TJ e CJ vs C) in the liver. These effects reduced IL-6 and TNF-α in the liver and colon, mRNA expression of IL-6R (CJ e TJ vs T; CJ vs C), TNF-��R1 (CJ e TJ vs T) and TLR-4 (CJ e TJ vs T) in the colon, increased of IL-10 in the RET and IL-10/TNF-α ratio in the liver (TJ vs T). Additionally, the jussara supplementation groups showed similar growth, less weight gain (CJ vs C, T and TJ) and better body composition, reflected by reduced lipid/protein ratio (CJ and TJ vs T) and higher carcass protein (TJ vs T). The jussara supplementation in maternal diet reduced lipids profile and glucose in offspring. Conclusion. The 0.5% Jussara supplementation prevented the adverse effects of TFAs, improved lipid profiles, glucose levels, body composition, restored gut microbiota and reduced low-grade inflammation in 21-day-old offspring via down-regulation of the NFkB signaling pathway. These findings suggests better paracellular barrier and could contribute to reduced inflammation and chronic disease development in later life.