Navegando por Palavras-chave "Tooth development"

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    Pluripotent stem cell transcription factors during human odontogenesis
    (Springer, 2013-09-01) Cunha, Juliana Malta da [UNIFESP]; Costa-Neves, Adriana da; Kerkis, Irina; Pereira da Silva, Marcelo Cavenaghi [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Inst Butantan
    Stem cells are capable of generating various cell lines and can be obtained from adult or embryonic tissues for clinical therapies. Stem cells from deciduous dental pulp are among those that are easily obtainable from adult tissues and have been widely studied because of their ability to differentiate into a variety of cell lines in the presence of various chemical mediators. We have analyze the expression of several proteins related to the differentiation and proliferative potential of cell populations that compose the tooth germ of human fetuses. We evaluate 20 human fetuses of both genders. After being paraffin-embedded, cap and bell stages of tooth germ development were subjected to immunohistochemistry for the following markers: Oct-4, Nanog, Stat-3 and Sox-2. the studied antibodies showed nuclear or cytoplasmic immunnostaining within various anatomical structures and with various degrees of expression, indicating the action of these proteins during tooth development. We conclude that the interrelationship between these transcription factors is complex and associated with self-renewal and cell differentiation. Our results suggest that the expression of Oct-4, Nanog, Sox-2 and Stat-3 are related to differentiation in ameloblasts and odontoblasts.
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    Tooth Tissue Engineering: Optimal Dental Stem Cell Harvest Based on Tooth Development
    (Wiley-Blackwell, 2011-07-01) Duailibi, Monica Talarico [UNIFESP]; Duailibi, Silvio Eduardo [UNIFESP]; Duailibi Neto, Eduardo Felippe; Negreiros, Renata Matalon; Jorge, Waldyr Antonio; Ferreira, Lydia Masako [UNIFESP]; Vacanti, Joseph Phillip; Yelick, Pamela Crotty; Tufts Univ; Universidade Federal de São Paulo (UNIFESP); Natl Inst Sci & Technol; Universidade de São Paulo (USP); Massachusetts Gen Hosp; Dept Surg; Harvard Univ
    Our long-term objective is to devise reliable methods to generate biological replacement teeth exhibiting the physical properties and functions of naturally formed human teeth. Previously, we demonstrated the successful use of tissue engineering approaches to generate small, bioengineered tooth crowns from harvested pig and rat postnatal dental stem cells (DSCs). To facilitate characterizations of human DSCs, we have developed a novel radiographic staging system to accurately correlate human third molar tooth developmental stage with anticipated harvested DSC yield. Our results demonstrated that DSC yields were higher in less developed teeth (Stages 1 and 2), and lower in more developed teeth (Stages 3, 4, and 5). the greatest cell yields and colony-forming units (CFUs) capability was obtained from Stages 1 and 2 tooth dental pulp. We conclude that radiographic developmental staging can be used to accurately assess the utility of harvested human teeth for future dental tissue engineering applications.