Navegando por Palavras-chave "TGF-beta 1"
Agora exibindo 1 - 4 de 4
Resultados por página
Opções de Ordenação
- ItemSomente MetadadadosEarly nuclear alterations and immunohistochemical expression of Ki-67, Erb-B2, vascular endothelial growth factor (VEGF), transforming growth factor (TGF-BETA 1) and integrine-linked kinase (ILK) two days after tamoxifen in breast carcinoma(Veda, Slovak Academy Sciences, 2004-01-01) Morena, Ana Maria Lira [UNIFESP]; Oshima, Celina Tizuko Fujiyama [UNIFESP]; Gebrim, Luiz Henrique [UNIFESP]; Egami, Mizue Imoto [UNIFESP]; Silva, Maria Regina Regis da [UNIFESP]; Segreto, Roberto Araujo [UNIFESP]; Giannotti Filho, Osvaldo [UNIFESP]; Teixeira, Vicente de Paulo Castro [UNIFESP]; Segreto, Helena Regina Comodo [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Oncoctr Fdn Sao PauloThe purpose of the present study was to evaluate breast carcinoma samples before and two days after treatment with tamoxifen in order to analyse early histopathological alterations - particularly nuclear alterations - as well as immunohistochemical expression of Ki-67, Erb-B2, VEGF, TGf-beta 1 and ILK proteins. Twenty one cases of invasive ductal and lobular breast carcinoma were studied. Patients were submitted to biopsy of the lesion and, after confirmation of the diagnosis, they received 20 mg of tamoxifen a day, beginning two days before surgery. The samples obtained during biopsy and after surgery were stained with HE for histopathological diagnosis. Estrogen receptor was positive in IS cases and negative in 3. The immunohistochemical method was applied for the detection of Ki-67, Erb-B2, protein, vascular endothelial growth factor (VEGF), transforming growth factor beta (TGF-beta 1) and integrin linked kinase (ILK).Two days after tamoxifen treatment, the following results were observed: 1) decrease in the cell volume, chomatine condensation, nucleoli less evident and clearly defined nuclear limits; 2) significant reduction in the expression of Erb-B2 protein and significant increase in the expression of TGF-beta 1 protein; 3) expression of others proteins (Ki-67, VEGF and ILK) was not altered during the indicated time frame.Our results suggest that analyzing nuclear alterations and expression of Erb-B2 and TGF-beta 1 proteins would be useful to assess the initial response to tamoxifen.
- ItemAcesso aberto (Open Access)Epithelium and stroma from nasal polyp mucosa exhibits inverse expression of TGF- beta(1) as compared with healthy nasal mucosa(Biomed Central Ltd, 2013-04-15) Balsalobre, Leonardo [UNIFESP]; Pezato, Rogerio [UNIFESP]; Perez-Novo, Claudina; Alves, Maria Teresa S. [UNIFESP]; Santos, Rodrigo P. [UNIFESP]; Bachert, Claus; Weckx, Luc L. M. [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Univ GhentObjective: To evaluate TGF-beta(1) expression in polypoid mucosa (epithelium and stroma) of patients with chronic rhinosinusitis with nasal polyposis (CRSwNP).Methods: Cross-sectional study with two groups: 17 patients with nasal polyposis and 11 controls. Polyps and normal nasal mucosa were processed by immunohistochemical methods for TGF-beta 1 visualization. Then, the percentage of TGF-beta 1 expression in stroma and epithelium was objectively quantified using UT Morph software.Results: A lower percentage of positive expression was found in the epithelium of CRSwNP patients (32.44%) versus normal controls (55.91%) (p < 0.05), and a higher percentage of positive expression in the stroma of CRSwNP patients (23.24%) versus controls (5.88%) (p < 0.05).Conclusion: the lower percentage of TGF-beta(1) expression in the nasal epithelium of CRSwNP patients may have an impact on epithelium-directed topical treatments employed in this patient population.
- ItemSomente MetadadadosFibrinolytic activity is associated with presence of cystic medial degeneration in aneurysms of the ascending aorta(Wiley-Blackwell, 2010-12-01) Borges, Luciano F. [UNIFESP]; Gomez, Delphine; Quintana, Mercedes; Touat, Ziad; Jondeau, Guillaume; Leclercq, Anne; Meilhac, Olivier; Jandrot-Perrus, Martine; Gutierrez, Paulo S.; Freymuller, Edna [UNIFESP]; Vranckx, Roger; Michel, Jean-Baptiste; Univ Paris 07; Universidade Federal de São Paulo (UNIFESP); Ctr Reference Syndrome Marfan & Syndromes Apparen; Universidade de São Paulo (USP)Fibrinolytic activity is associated with presence of cystic medial degeneration in aneurysms of the ascending aortaAims:Thoracic ascending aortic aneurysms (TAA) are characterized by elastic fibre breakdown and cystic medial degeneration within the aortic media, associated with progressive smooth muscle cell (SMC) rarefaction. the transforming growth factor (TGF)-beta/Smad2 signalling pathway is involved in this process. Because the pericellular fibrinolytic system activation is able to degrade adhesive proteins, activate matrix metalloproteinase (MMP), induce SMC disappearance and increase the bioavailability of TGF-beta, the aim was to investigate the plasminergic system in TAA.Methods and results:Ascending aortas [21 controls and 19 TAAs (of three different aetiologies)] were analysed. Immunohistochemistry showed accumulation of t-PA, u-PA and plasmin in TAAs, associated with residual SMCs. Overexpression of t-PA and u-PA was confirmed by reverse transcription-polymerase chain reaction (RT-PCR), immunoblotting and zymography on TAA extracts and culture medium conditioned by TAA. Plasminogen was present on the SMC surface and inside cytoplasmic vesicles, but plasminogen mRNA was undetectable in the TAA medial layer. Plasmin-antiplasmin complexes were detected in TAA-conditioned medium and activation of the fibrinolytic system was associated with increased fibronectin turnover. Fibronectin-related material was detected immunohistochamically in dense clumps around SMCs and colocalized with latent TGF-beta binding protein-1.Conclusions:The fibrinolytic pathway could play a critical role in TAA progression, via direct or indirect impact on ECM and consecutive modulation of TGF-beta bioavailability.
- ItemSomente MetadadadosRole of chymase in diabetic nephropathy(Royal Soc Medicine Press Ltd, 2012-08-01) Cristovam, Priscila C. [UNIFESP]; Carmona, Adriana K. [UNIFESP]; Arnoni, Carine P. [UNIFESP]; Maquigussa, Edgar [UNIFESP]; Pereira, Luciana G. [UNIFESP]; Boim, Mirian A. [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Chymase is an alternative pathway for angiotensin-converting enzyme in angiotensin II (Ang II) formation, and its expression is increased in human diabetic kidneys and in human mesangial cells (MCs) stimulated with high glucose. in addition, chymase activates transforming growth factor (TGF-beta 1) via an Ang II-independent pathway. the aim of this study was to evaluate the role of chymase on TGF-beta 1 activation in diabetic rats and in rat MCs (RMCs) stimulated with high glucose (HG). Diabetes was induced in male Wistar rats by streptozotocin (60 mg/kg, intravenous). After 30 (D30) or 60 (D60) days, chymase activity and the expression of profibrotic markers were evaluated. RMCs were stimulated with HG in the presence or absence of 50 mu mol/L chymostatin, a chymase inhibitor, or 100 nmol/L of losartan, an Ang II antagonist. Chymase activity and expression increased in 060 kidneys, with increased expression of fibronectin, type I and III collagen, TGF-beta 1 and Smad 3 and with no change in Smad 7 expression. RMCs exposed to HG presented increases in chymase activity and expression, together with upregulation in fibrosis markers and in the TGF-beta 1 signaling pathway. All these effects were reversed by chymostatin and by losartan, but type 1 angiotensin II receptor blockade did not interfere with the Smad 3 and 7 pathway. Similar to HG-stimulated RMCs, control RMCs treated with chymase responded with increased expression of TGF-beta 1, Smad 3 and fibrosis markers. These effects were reversed by chymostatin but not by losartan. the results indicate an important role for chymase in inducing fibrosis through TGF-beta 1 activation, parallel with Ang II effects.