Navegando por Palavras-chave "T-lymphocytes"
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- ItemSomente MetadadadosAção do levamiosole sobre linfócitos T "in vitro"(Universidade Federal de São Paulo (UNIFESP), 1977) Succi, Regina Célia de Menezes [UNIFESP]; Naspitz, Charles Kirov [UNIFESP]
- ItemAcesso aberto (Open Access)Avaliação de células mielóides supressoras e linfócitos T em indivíduos longevos(Universidade Federal de São Paulo (UNIFESP), 2018-02-22) Alves, Amanda Soares [UNIFESP]; Bueno, Valquiria [UNIFESP]; http://lattes.cnpq.br/1984756436508114; http://lattes.cnpq.br/3901894720824067; Universidade Federal de São Paulo (UNIFESP)Modern society has been facing a major demographic revolution that is the population aging. The increase in life expectancy depends on the adjusted function of several organs and tissues in order to deal with damaging events in life time. Successful aging seems to be dependent at least in part on the adequate function of the immune system. It has been reported that aging is associated with changes in the percentage of myeloid-derived suppressor cells (MDSC) and T lymphocytes besides the impairment of T cells functions. Our aim was to evaluate young and long-living individuals according to myeloid-derived suppressor cells; T cells proliferative response, phenotype, and cytokines secretion after mitogen stimulation. Non-institutionalized both gender long-living individuals (80 years old and over; 80+) from SABE study were evaluated and compared to young students from UNIFESP (20-30 years old). Aging was associated with reduced circulating number of leukocytes in older individuals (80+). The percentage of MDSC was higher in 80+ group whereas the absolute number of these cells was not statistically different when young and 80+ individuals were compared. Aged individuals also presented higher CD4/CD8 ratio, decrease of naïve and increase of EMRA cells mainly in CD8+ compartment. Under PHA stimulus 80+ individuals presented lower proliferative capacity, and decreased expression of IL-1, IL-2, IL-6, IFN-y, and TNF-alpha. Aging is a complex, multifactorial, and heterogeneous process and based on our results it is suggested that MDSC (percentage and absolute cell number), percentage of naïve and EMRA TCD8+ cells, CD4/CD8 ratio, proliferative T cell percentage, and cytokine levels could be used as biomarkers in aging individuals to predict pathologies, to indicate interventions and to evaluate interventions' efficacy. In addition, aging population presented very heterogeneous results, reinforcing the importance of an individualized treatment for these individuals.
- ItemAcesso aberto (Open Access)Avaliação fenotípica das células T reguladoras CD4+CD25+CD127LOW em pacientes com lúpus eritematoso sistêmico(Universidade Federal de São Paulo (UNIFESP), 2009-04-29) Mesquita Júnior, Danilo [UNIFESP]; Andrade, Luiz Eduardo Coelho [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Systemic lupus erythematosus (SLE) is a chronic inflammatory disease that is part of the group of rheumatic autoimmune inflammatory diseases, being characterized by heterogeneous clinical and laboratory manifestations. The exact etiopathogenic mechanism underlying SLE still remains obscure. Previousr observations evaluating CD4+ CD25+ TREG cell function in auto-immune diseases detected alterations on frequency and on phenotypic and functional features in murine and human models that support the significant activity of this cell population on autoimmune pathophysiology. In SLE we can observe the existence of a complex interaction network that characterizes the disease, in which many targets for therapeutic intervention may be considered. The present study has focused on TREG cells, since they may represent putative targets for immunomodulatory therapy in this disease. Published data on frequency and phenotype of TREG cells is controversial due to heterogeneity of phenotypic markers and analytic strategies used. The present project aimed to validate an appropriate strategy to identify and quantify TREG in SLE. The CD4+CD25highCD127 low/- panel was validated as an appropriate strategy for identification of Foxp3+ TREG cells in healthy and in SLE patients. The frequency of TREG cells presented normal frequency in active and inactive SLE. In contrast, the frequency of conventional non-regulatory T cells was increased in patients with active disease. We also evaluated the expression of important phenotypic markers for TREG cells biology, including CTLA-4, GITR, PD-1, OX40, HLA-DR, CD95, CD45RO, CD28 and CD40L in patients with active and inactive disease. In addition we evaluated the relationship between the balance of TREG cells versus conventional non-regulatory T cells expressing these markers by means of deriving the TREG/Tconv rate for each surface marker. In patients with active disease we observe reduced levels of TREG cells expressing CTLA-4 and CD28 molecules, and elevated levels of CD40L+ TREG cells. There was an imbalance in TREG/Tconv for GITR, HLA-DR, OX40, CD40L and CD45RO: samples from active SLE patients depicted a decreased TREG/Tconv ratio for GITR, HLA-DR, OX40 and CD45RO and an increased ratto for CD40L when compared with healthy controls. The knowledge on the role of TREG cells in SLE may bring important contribution in devising therapeutic alternatives for this disease.
- ItemSomente MetadadadosIdentificação de linfócitos T e B em conjuntivites foliculares e na síndrome de Sjogren(Universidade Federal de São Paulo (UNIFESP), 1978) Belfort, Rubens Junior [UNIFESP]; Mendes, Nelson Figueiredo [UNIFESP]