Navegando por Palavras-chave "Sulfato De Glicosamina"

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    Efeitos Da Fotobiomodulação E Dos Sulfatos De Glicosamina E Condroitina (Associados Ou Não) Na Cartilagem Articular Em Um Modelo Experimental De Osteoartrite Em Ratos
    (Universidade Federal de São Paulo (UNIFESP), 2017-09-01) Barros, Marcella Sanches Paes De [UNIFESP]; Renno, Ana Claudia Muniz [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Osteoarthritis (OA) is a degenerative joint disease prevalent in the elderly population and is most frequent in the knee joint. Currently, the therapeutic potential of photobiomodulation (FBM) and chondroprotectors, such as the glucosamine and chondroitin sulfates, is highlighted in the treatment of degenerative diseases affecting the joint cartilage. However there is no evidence of concomitant use of these treatments and what the possible interaction between them. Thus, the hypothesis supported was that the association of glucosamine and chondroitin sulfates to FBM may constitute a more effective therapeutic intervention to reduce morphological alterations in the joint cartilage associated with OA. In this way, the objective of this study was to evaluate the effects of FBM and glucosamine and chondroitin sulfates, associated or not, on the rat knee joint cartilage in an experimental model of OA by transection of the anterior cruciate ligament (TLCA). 43 male Wistar rats, separated into 5 groups: Control without lesion (GC); Control model of osteoarthritis - OA without treatment (OA); Model of OA and submitted to the FBM (OAL); Model of OA and treated with the combination of glucosamine and chondroitin sulfates (OAS); Model of OA, submitted to FBM and treated with glucosamine and chondroitin sulfates (OASL). The treatment started 48 hours after surgery for 29 consecutive days. A combination of glucosamine sulfate (500 mg/kg) and chondroitin sulfate (400 mg/kg), and FBM (AsGaAl, continuous = λ=808 nm, P=50 mW, beam area=0.028 cm 2, t= 28s, D=50 J/cm2; = 1.4 J, irradiance=17.8 mW/cm2) at 2 points of the left pelvic limb (lateral and medial face joint). The animals were euthanized 31 days after the surgical procedure. In order to evaluate and compare the effects of the treatments, qualitative histological analyzes were performed using the International Society of OA (OARSI) criteria, morphometric thickness and chondrocyte density, as well as analysis of the expression of Interleukin 1β (IL-1β) and 10 (IL-10), and type II collagen. The results of the present study showed that all treated groups presented better structural organization of joint cartilage and reduction of chondrocyte density. In addition, the OAS and OASL groups presented lower values in OARSI when compared to the other groups. Moreover, the OASL group showed a reduction in IL- 1β immunoexpression and an increase in the immunoexpression of IL-10 and type II collagen when compared to OA. Therefore, we can conclude that the glucosamine sulfate and chondroitin sulfate compound were effective in preventing cartilage degeneration, as well as its association with FBM promoted positive effects in the expression of factors related to the inflammatory process in the experimental model used.