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    Desenvolvimento e validação de método analítico para padronização do extrato Mouriri elliptica Martius e avaliação do extrato seco por Spray Dryer.
    (Universidade Federal de São Paulo, 2018-09-06) Almeida, Rosinês da Silva [UNIFESP]; Andreo, Marcio Adriano [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    The Mouriri elliptica (Melastomataceae) leaves, also known as "croadinha", presented considerable activity in peptic ulcer and gastritis healing. This action was validated by traditional use and confirmed with scientific studies. The majority compounds found in this species are flavonoids myricetin, quercetin and their derivatives. Aiming the use of this species as a phytotherapic medicine, this work standardized the leaves ethanolic extract, quantifying the major compounds for quality control and analyzed the drying process of the extract. Myricetin and quercetin may be possible markers, however, the large number of their glycosidic derivatives present in the extract makes it difficult to quantify them, hydrolysis step is required. The work proposed an optimization of the acid hydrolysis process, using factorial planning complete to investigate the effects of HCl concentration and reaction time. The ideal conditions found were: 2 mol HCl and 40 minutes reaction time. After defining the condition of hydrolysis of flavonoid myricetin and quercetin, was developed and validated a method for the quantification of these compounds. Analyzes by HPLC The developed method presented linearity (R2 = 0,996 for myricetin and R2 = 0,997 for quercetin). Besides, the chromatographic peaks showed good resolution. With other validation data, including precision, specificity and accuracy, this method demonstrated good reliability and sensitivity. This method was applied for quantification of myricetin and quercetin, and the results were: 54 μg/mL myricetin and 12 μg/mL quercetina. Together with the quality control testing the content was determined of 1,52 % myricetin and 0,32 % of quercetin in the ethanolic extract of the leaves of M. elliptica. The drying process of standardized extract was performed in spray dryer, using complete factorial planning 23 . The variables were inlet temperature, % Aerosil and flow. Dry extract samples were subjected to analysis of recovery of flavonoids, yield and moisture. The optimized conditions based on the analysis of desirability were: 160 °C inlet temperature, Aerosil 50% and 2mL/min flow. Obtaining an average content of 14.79 μg / mL myricetin and 3.35 μg / mL quercetin.