Navegando por Palavras-chave "Sporothrix schenckii complex"

Agora exibindo 1 - 3 de 3
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Characterization of virulence profile, protein secretion and immunogenicity of different Sporothrix schenckii sensu stricto isolates compared with S. globosa and S. brasiliensis species
    (Landes Bioscience, 2013-04-01) Fernandes, Geisa Ferreira [UNIFESP]; Santos, Priscila Oliveira dos [UNIFESP]; Rodrigues, Anderson Messias [UNIFESP]; Sasaki, Alexandre Augusto [UNIFESP]; Burger, Eva; Camargo, Zoilo Pires de [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Univ Fed Alfenas
    A comparative study about protein secretion, immunogenicity and virulence was performed in order to characterize and to compare eight Sporothrix schenckii sensu stricto isolates. for virulence characterization, a murine model, based on survival assay and CFU counting was used. S. brasiliensis and S. globosa, a highly virulent and a non-virulent isolates, respectively were used as external controls. Exoantigen profiles showed different secreted molecules; the 46- and 60-kDa molecules were commonly secreted by all three species. the S. schenckii s. str. isolates could be classified as non-virulent or presenting low, medium or high virulence, based on survival times after infection and recovery of viable fungi. the humoral response profiles of mice infected with S. schenckii s. str., S. globosa and S. brasiliensis were heterogeneous; five virulent isolates (S. schenckii s. str., n = 4 and S. brasiliensis, n = 1) had in common the recognition of the 60-kDa molecule by their respective antisera, suggesting that this antigen may be involved in virulence. Furthermore, the 110-kDa molecule was secreted and recognized by antisera from four virulent isolates (S. schenckii s. str., n = 3 and S. brasiliensis, n = 1), so there is a possibility that this molecule is also related to virulence. Our findings reveal different degrees of virulence in S. schenckii s. str. isolates and suggest the correlation of protein secretion and immunogenicity with virulence of S. schenckii complex. These findings provide new insights into the pathogenesis of S. schenckii s. str. and improve the knowledge about immunogenicity and protein profiles in S. schenckii complex.
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Emergence of pathogenicity in the Sporothrix schenckii complex
    (Informa Healthcare, 2013-05-01) Rodrigues, Anderson Messias [UNIFESP]; De Hoog, Sybren; Camargo, Zoilo Pires de [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); CBS KNAW Fungal Biodivers Ctr
    Sporothrix schenckii sensu lato is a complex of thermally dimorphic species whose natural habitats are soil and plant materials. However, the traumatic implantation of the species into human skin is traditionally thought to be the route leading to the fungal disease sporotrichosis. the complex contains Sporotrhix mexicana, S. globosa, S. brasiliensis, S. luriei, in addition to S. schenckii sensu stricto. in this study we evaluated the differences among these species relative to their frequency in the environment and in human hosts, as well as discuss their remarkable diverse pathogenicity. Today, S. brasiliensis is epidemic in and geographically restricted to Brazil. in contrast, S. mexicana and S. globosa have rarely been reported over the decades. We discovered that the species have been present in collections from clinical cases since 1955 and were able to re-identify six isolates originally classified as S. schenckii as Sporothrix mexicana (three isolates) and Sporothrix globosa (three isolates). Despite their long presence as potential human pathogens they have not shown any increase in frequency as etiologic agents of human infections.
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    In vitro susceptibility of antifungal drugs against Sporothrix brasiliensis recovered from cats with sporotrichosis in Brazil
    (Oxford Univ Press, 2016) Nogueira Brilhante, Raimunda Samia [UNIFESP]; Rodrigues, Anderson Messias [UNIFESP]; Costa Sidrim, Jose Julio [UNIFESP]; Gadelha Rocha, Marcos Fabio [UNIFESP]; Pereira, Sandro Antonio; Ferreira Gremiao, Isabella Dib; Pacheco Schubach, Tania Maria; de Camargo, Zoilo Pires [UNIFESP]
    Sporotrichosis is an important subcutaneous mycosis of humans and animals. Classically, the disease is acquired upon traumatic inoculation of Sporothrix propagules from contaminated soil and plant debris. In addition, the direct horizontal transmission of Sporothrix among animals and the resulting zoonotic infection in humans highlight an alternative and efficient rout of transmission through biting and scratching. Sporothrix brasiliensis is themost virulent species of the Sporothrix schenckii complex and is responsible for the long-lasting outbreak of feline sporotrichosis in Brazil. However, antifungal susceptibility data of animal-borne isolates is scarce. Therefore, this study evaluated the in vitro activity of amphotericin B, caspofungin, itraconazole, voriconazole, fluconazole, and ketoconazole against animal-borne isolates of S. brasiliensis. The susceptibility tests were performed through broth microdilution (M38-A2). The results show the relevant activity of itraconazole, amphotericin B, and ketoconazole against S. brasiliensis, with the following MIC ranges: 0.125-2, 0.125-4 and 0.0312-2 mu g/ml, respectively. Caspofungin was moderately effective, displaying higher variation in MIC values (0.25-64 mu g/ml). Voriconazole (2-64 mu g/ml) and fluconazole (62.5-500 mu g/ml) showed low activity against S. brasiliensis strains. This study contributed to the characterization of the in vitro antifungal susceptibility of strains of S. brasiliensis recovered from cats with sporotrichosis, which have recently been considered the main source of human infections.