Navegando por Palavras-chave "Sjogren's syndrome"
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- ItemSomente MetadadadosAnti-aquaporin-4 antibodies in the context of assorted immune-mediated diseases(Wiley-Blackwell, 2012-02-01) Dellavance, A.; Alvarenga, R. R. [UNIFESP]; Rodrigues, S. H. [UNIFESP]; Kok, F.; Souza, A. W. S. de [UNIFESP]; Andrade, L. E. C. [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Fleury Grp; Universidade de São Paulo (USP)Background and purposes: Anti-aquaporin 4 antibodies are specific markers for Devics disease. This study aimed to test if this high specificity holds in the context of a large spectrum of systemic autoimmune and non-autoimmune diseases.Methods: Anti-aquaporin-4 antibodies (NMO-IgG) were determined by indirect immunofluorescence (IIF) on mouse cerebellum in 673 samples, as follows: group I (clinically defined Devic's disease, n = 47); group II [ inflammatory/demyelinating central nervous system (CNS) diseases, n = 41]; group III (systemic and organ-specific autoimmune diseases, n = 250); group IV (chronic or acute viral diseases, n = 35); and group V (randomly selected samples from a general clinical laboratory, n = 300).Results: MNO-IgG was present in 40/47 patients with classic Devic's disease (85.1% sensitivity) and in 13/22 (59.1%) patients with disorders related to Devic's disease. the latter 13 positive samples had diagnosis of longitudinally extensive transverse myelitis (n = 10) and isolated idiopathic optic neuritis (n = 3). One patient with multiple sclerosis and none of the remaining 602 samples with autoimmune and miscellaneous diseases presented NMO-IgG (99.8% specificity). the autoimmune disease subset included five systemic lupus erythematosus individuals with isolated or combined optic neuritis and myelitis and four primary Sjogren's syndrome (SS) patients with cranial/peripheral neuropathy.Conclusions: the available data clearly point to the high specificity of anti-aquaporin-4 antibodies for Devic's disease and related syndromes also in the context of miscellaneous non-neurologic autoimmune and non-autoimmune disorders.
- ItemAcesso aberto (Open Access)Assessment of fatigue and dryness in primary Sjogren's syndrome: Brazilian version of Profile of Fatigue and Discomfort - Sicca Symptoms Inventory (short form) (PROFAD-SSI-SF)(Elsevier B.V., 2015-03-01) Miyamoto, Samira Tatiyama [UNIFESP]; Paganotti, Mauricio Aquino; Serrano, Erica Vieira; Giovelli, Raquel Altoé; Valim, Valeria [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Univ Fed Espirito Santo; Univ Vila Velha; Escola Super CienciasObjective: To perform a cross-cultural adaptation and validation of the Profile of Fatigue and Discomfort - Sicca Symptoms Inventory (short form) (PROFAD-SSI-SF) questionnaire assessing the subjective aspects of the symptoms of primary Sjogren syndrome (pSS), for the Brazilian Portuguese language.Method: Conceptual, of the item, semantic and operational equivalences were evaluated. the Brazilian version of PROFAD-SSI-SF was administered to 62 women with pSS according to the European-American consensus 2002 to assess measurement equivalence. alpha-Cronbach was used for internal consistency; intraclass correlation coefficient (ICC) for intraobserver reproducibility; and Spearman correlation coefficient for validity by comparing with Patient Global Assessment (PaGA), EULAR Sjogren's Syndrome Patient Reported Index (ESSPRI), Functional Assessment of Chronic Illness Therapy Fatigue Subscale (FACIT-F) and EuroQOL (EQ-5D).Results: the internal consistency of PROFAD, SSI and total score was 0.80; 0.78; and 0.87, respectively. the intraobserver reproducibility of total PROFAD was 0.89; of total SSI of 0.86; and total score of 0.89. in terms of validity, PROFAD correlated significantly with PaGA (r = 0.50), FACIT-F (r = 0.59), ESSPRI (r = 0.58) and all domains of EQ-5D, with the exception of Mobility. On the other hand, SSI correlated significantly with PaGA (r = 0.43), FACIT-F (r = 0.57), ESSPRI (r = 0.55) and most areas of EQ-5D. the total score of PROFAD-SSI-SF had a non-statistically significant correlation only with Mobility domain and with 1-100 range of EQ-5D.Conclusion: the Portuguese version of PROFAD-SSI-SF proved to be an adaptable, reproducible and valid tool for the Brazilian Portuguese language. (C) 2014 Elsevier Editora Ltda. All rights reserved.
- ItemSomente MetadadadosAutoantibodies to 60 kDa SS-A/Ro yield a specific nuclear myriad discrete fine speckled immunofluorescence pattern(Elsevier B.V., 2013-04-01) Dellavance, Alessandra; Alvarenga, Rossana Rassi [UNIFESP]; Rodrigues, Silvia Helena [UNIFESP]; Barbosa, Silvia Helena; Pinto Camilo, Amandia Cristina; Okamoto Shiguedomi, Herika Santiago; Rodrigues, Silvia Sanchez; Silva, Cristiane Gallindo; Coelho Andrade, Luis Eduardo [UNIFESP]; Fleury Med & Hlth Labs; Universidade Federal de São Paulo (UNIFESP)Objectives: To report on a novel immunofluorescence pattern specifically associated with antibodies to SS-A/Ro.Methods: A novel immunofluorescence pattern, herein designated SS-A/Ro pattern, was characterized as myriad discrete fine speckles throughout the nucleus. Eighty-six sequential samples presenting the SS-A/Ro pattern and 64 samples presenting non-SS-A/Ro nuclear fine speckled pattern at the ANA-HEp-2 routine were screened for SS-A/Ro reactivity. Conversely, 48 samples with known reactivity to 60 kDa SS-A/Ro, 13 samples with exclusive reactivity to 52 kDa SS-A/Ro, and 48 SS-A/Ro-negative samples were analyzed for the ANA-HEp-2 pattern.Results: Eighty-five of the 86 samples (98.8%) presenting the SS-A/Ro pattern and 15 of the 64 (23.4%) samples with non-SS-A/Ro fine speckled pattern reacted with 60 kDa SS-A/Ro. Conversely, 44 (91.6%) of 48 samples with known reactivity to 60 kDa SS-A/Ro presented the SS-A/Ro pattern and four (8.4%) presented non-SS-A/Ro fine speckled pattern. None of the 48 anti-SS-A/Ro-negative samples and none of anti-52 kDa SS-A/Ro-positive samples yielded the SS-A/Ro pattern. This immunofluorescence pattern was observed in different commercial HEp-2 cell slides and in homemade HEp-2 cell slides.Conclusions: the SS-A/Ro pattern belongs to the group of immunofluorescence patterns that hold strong association with the respective autoantibody specificities, such as those associated with CENP-F and NuMA-1. the identification of the SS-A/Ro pattern at the ANA-HEp-2 screening routine shall lead to specific tests for the identification of anti-SS-A/Ro antibodies. (C) 2013 Elsevier B.V. All rights reserved.
- ItemSomente MetadadadosDifferential expression of Ro/SSA 60 kDa and La/SSB, but not Ro/SSA 52 kDa, mRNA and protein in minor salivary glands from patients with primary Sjogren's syndrome(J Rheumatol Publ Co, 2007-06-01) Barcellos, Karin S. A. [UNIFESP]; Nonogaki, Suely [UNIFESP]; Enokihara, Milvia M. S. S. [UNIFESP]; Teixeira, Miriam S. [UNIFESP]; Andrade, Luis E. C. [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Adolpho Lutz InstObjective. To analyze the protein and messenger RNA (mRNA) expression of La/SSB, Ro/SSA 60, and Ro/SSA 52 antigens in minor salivary glands (MSG) from patients with primary Sjogren's syndrome (pSS).Methods. La/SSB, Ro/SSA 60, and Ro/SSA 52 protein expression was studied by immunohistochemistry in MSG from 26 patients with pSS and 16 controls. mRNA expression was determined by real-time polymerase chain reaction in MSG of 10 patients with pSS and 7 controls.Results. La/SSB and Ro/SSA 60, but not Ro/SSA 52, mRNA expression was higher in samples from patients with pSS compared to controls (p < 0.05). La/SSB protein had higher expression in the cytoplasm of ductal cells than in the cytoplasm of mucous acinar cells in patients with pSS (p = 0.013) but not in controls. Ro/SSA 60 had higher expression in the cytoplasm of ductal cells than in the cytoplasm of serous acinar cells in patients with pSS (p = 0.006) but not in controls. The Ro/SSA 52 expression pattern was similar in patients and controls. There was no association between circulating autoantibodies to Ro/SSA or La/SSB and the aberrant expression of the cognate autoantigens.Conclusion. The increased Ro/SSA 60 and La/SSB mRNA expression in MSG of patients with pSS as well as the differential Ro/SSA 60 and La/SSB protein expression in ductal cells of MSG in patients with pSS suggest that these these 2 autoantigens, but not Ro/SSA 52, are probably involved in triggering and maintaining the tissue-specific autoimmune response in pSS MSG and may contribute to the antigen-driven immune response and local autoantibody production
- ItemSomente MetadadadosIdentificação de linfócitos T e B em conjuntivites foliculares e na síndrome de Sjogren(Universidade Federal de São Paulo (UNIFESP), 1978) Belfort, Rubens Junior [UNIFESP]; Mendes, Nelson Figueiredo [UNIFESP]
- ItemSomente MetadadadosLow-level laser therapy for xerostomia in primary Sjogren's syndrome: a randomized trial(Springer London Ltd, 2018) Fidelix, Tania [UNIFESP]; Czapkowski, Adriano [UNIFESP]; Azjen, Sergio [UNIFESP]; Andriolo, Adagmar [UNIFESP]; Horvath Neto, Pedro [UNIFESP]; Trevisani, Virgínia Fernandes Moça [UNIFESP]To evaluate the effectiveness of low-level laser therapy (LLLT) in the treatment of xerostomia in primary Sjogren's syndrome (SS), a randomized clinical trial of patients with dry mouth symptoms associated with primary SS receiving care at a university hospital was conducted. Sixty-six patients were randomly assigned with a 1:1 allocation ratio to receive LLLT (laser group, n = 33) or placebo treatment (placebo group, n = 33). Patients in the laser group received LLLT twice a week for 6 weeks, for a total of 12 treatment sessions. Laser irradiation was performed with an aluminum-gallium-arsenide laser diode at a wavelength of 808 nm, 100-mW output power, and energy density of 4.0 J/cm(2) per irradiation point per session. Placebo treatment was performed following the same protocol used for irradiated patients and using the same laser device to mimic a real irradiation, but with no active laser emission and the tip of the laser probe covered with aluminum foil. The outcomes of interest were xerostomia inventory scores, salivary flow rate, salivary beta-2 microglobulin levels, and salivary sodium and chlorine concentrations. Patients in both groups showed no improvement in xerostomia. Likewise, there was no significant improvement in xerostomia inventory scores (p = 0.301) or salivary flow rate (p = 0.643) in either group. There was no difference in salivary beta-2 microglobulin levels, sodium concentration, and chlorine concentration before and after intervention or between the two groups. The LLLT protocol used in this study effected no improvement in xerostomia or salivary flow rate in patients with primary SS. ClinicalTrials.gov Identifier: NCT02066896.