Navegando por Palavras-chave "Scav-1"

Agora exibindo 1 - 1 de 1
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Caracterização fenotípica do mutante Scav-1 de caenorhabditis elegans
    (Universidade Federal de São Paulo (UNIFESP), 2020-07-30) Silva, Rafaella Cecilia Da [UNIFESP]; Cunha, Fernanda Marques Da [UNIFESP]; Universidade Federal de São Paulo
    CD36 is a scavenger receptor involved in several homeostatic processes of mammals such as, for example, control of angiogenesis, the innate immune response and lipid metabolism. Although the functions of CD36 and the molecular pathways involved in angiogenesis and the immune response are well characterized, there is still much to be defined about, for example, the role of CD36 in lipid metabolism. CD36 has been implicated in the deregulated metabolism of fatty acids under pathophysiological conditions. Data gathered so far indicate that CD36 has a wide participation in lipid metabolism, being involved in aspects ranging from the absorption of lipids from the diet to the composition of the cellular lipidome. However, the molecular aspects of CD36 function are not clear. Historically, the use of organisms simpler than rodents such as the nematode C. elegans has been of great value in elucidating complex biological processes at the molecular level. Thus, the present study aimed to characterize basic aspects of the biology of C. elegans mutants for scav-1, a possible CD36 orthologue in the worm. For this, analyzes of fertility, pharynx pumping rate, mobility, life span, development time and biometric measurements were carried out. In addition, proteins and carbohydrates of these animals were quantified. The data obtained show that the absence of SCAV-1 delays the development of the animal and impacts on reproduction, decreasing the number of eggs laid by hermaphrodites. Health parameters such as pharynx pumping and body movement were affected by the absence of SCAV-1, especially during aging, suggesting that the absence of SCAV-1 leads to premature aging. Corroborating this hypothesis, longevity data indicate that scav-1 mutants have a significantly reduced mean lifespan when compared to wild type worms. In addition, it was observed that the mutation leads to reduced body protein content, but does not alter the level of carbohydrates. Together, the results show that SCAV-1 is fundamental for several aspects of the biology of C. elegans