Navegando por Palavras-chave "Síndrome De Behçet"

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    Subpopulações de monócitos no sangue periférico de pacientes com Doença de Behçet – há associação com manifestações clínicas e atividade de doença?
    (Universidade Federal de São Paulo (UNIFESP), 2021) Del Padre, Talita Cardoso Gazzito [UNIFESP]; Souza, Alexandre Wagner Silva De [UNIFESP]; Universidade Federal de São Paulo
    Introduction: Behçet's disease (BD) is a systemic inflammatory disease, classified as variable vessel vasculitis, characterized by recurrent episodes of mucocutaneous, ocular, joint, vascular, gastrointestinal, and neurological manifestations. Monocytes participate in the activation of neutrophils in BD; however, no study has assessed changes in the distribution of monocyte subpopulations in peripheral blood of BD patients. Objective: To evaluate the frequency and absolute number of classical, intermediate, and non-classical monocytes in the peripheral blood of BD patients and in healthy controls. In addition, associations between monocyte subpopulations in peripheral blood and disease activity, severity of clinical manifestations and therapy were analyzed. Methods: A cross-sectional study was carried out including 49 BD patients, 28.6% of them with active disease, and 39 healthy controls. Flow cytometry was performed to assess monocyte subpopulations in peripheral blood with the following markers: CD14, CD16 and CD66b. The monocyte subpopulations were subdivided into classical (CD14+CD16-), intermediate (CD14+CD16dim) and non-classical (CD14dimCD16high). CD66b was used to exclude neutrophils from the analysis. Results were expressed as median and interquartile range of the number of cells x 106/L and as a percentage of cells over the total number of monocytes. Results: A relationship was observed between BD and a lower absolute number of monocytes [349.16 (279.40-408.66) vs. 439.25 (312.81-532.58); p = 0.020] and classical monocytes [275.38 (175.50-322.07) vs. 380.80 (279.92-499.17); p <0.0001], in addition to a lower percentage of classical monocytes [84.7% (76.4-91.2) vs. 89.5% (86.0-94.0); p = 0.002] compared to the control group, respectively. On the other hand, BD was related to a higher number [30.46 (20.25-66.29) vs. 21.08 (13.48-25.64); p <0.0001] and percentage [9.2% (5.5, -16.9) vs. 4.9% (3.0-6.7); p < 0.0001] of intermediate monocytes in peripheral blood compared to the control group, respectively. There were no differences between BD and controls regarding non-classical monocytes in peripheral blood. In BD, there was no relationship between subpopulations of monocytes in peripheral blood and xii disease activity, severe manifestations of BD or glucocorticoid therapy, immunosuppressive or biological agents. However, the use of colchicine was been associated with a higher number of non-classical monocytes in peripheral blood [41.21 (11.47-59.04) vs. 15.19 (9.92-22.53); p = 0.035]. Conclusion: BD patients presented an altered distribution of monocyte subpopulations in peripheral blood as compared to healthy controls with a lower number of total monocytes, a lower number and percentage of classical monocytes and a higher number and percentage of intermediate monocytes. An increase in the number of non-classical monocytes was associated with the use of colchicine in BD.