Navegando por Palavras-chave "Respiratory Diseases"

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    CD 133, marcador de células progenitoras na polipose nasal e suas relações com IL-12p40, IL12p70 e Il-8
    (Universidade Federal de São Paulo (UNIFESP), 2020-01-30) Lino, Wagner Vargas Souza [UNIFESP]; Pezato, Rogerio [UNIFESP]; Universidade Federal de São Paulo
    Introduction: Nasal Polyposis is à disease based on two pillars: chronic inflammatory process and altered remodeling process that leads to mechanical dysfunction. The stem cells have the potential to mitigate the inflammatory process and tissue regeneration. The PN derived stem cells shows gene expression related to protein grant increased when compared to healthy nasal mucosa, such as CD133, increased the healthy nasal mucosa. CD133 is a marker of progenitor cells, associated with stem cells and tissue growth and neoplasia. Therefore, this led us to investigate the concentration of CD133 in polypoid tissue and its relationships with major inflammatory interleukins. Objective: To evaluate CD133 protein expression in nasal polypoid tissue and its relationship with IL-12P40, IL-12p70 and IL-8. Method: The sample consisted of 74 participants, divided into two groups: one of patients with nasal polyposis (n = 39) according to EPOS 2012 criteria and a control group without inflammatory nasal mucosa disease (n = 35). It was measured in nasal polyp tissue in the Nasal Polyposis group and in the middle meatus mucosa of the control group: TGF-β by multiplex and IL-8, IL-12p40 and IL-12p70, CD133 by the Elisa method. The presence of eosinophils was evaluated by immunohistochemistry. Results: The eosinophil count in the Nasal Polyp sample was above 5 eosinophils per High-Power Field in 91% and in 73% of the cases presented more than 10 eosinophils per HighPower Field. CD133 and TGF-β levels were significantly lower in the polypoid tissue group when compared to healthy nasal tissues from middle meatus. Dosage of interleukins IL-8, IL-12p40 and IL-12p70 were significantly lower in the group with nasal polyps when compared to the control group. There was a significant positive correlation between CD133 and IL-8, IL-12p40, IL-12p70 in the control patients, and this correlation was weakened in patients with nasal polyposis and especially in aspirin intolerant patients. There was no correlation between CD133 and TGF-β regardless of which group compared. Conclusion: CD133 and IL-8, IL-12p40 and IL12p70 are decreased in nasal polyps tissue compared to normal mucosa, also in nasal polyposis there was weakening of positive correlations found between CD133 and IL-12. 8, IL-12p40 and IL-12p70 in normal mucosal tissue. These findings demonstrate the difficulty of nasal polypoid tissue to adequately respond to the increase in inflammatory interleukins through its progenitor cells, since this tissue does not have a predictable response to related Th interleukins.