Navegando por Palavras-chave "Recombinant"

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    Clonagem, Expressão E Purificação De Bmp4 Recombinante
    (Universidade Federal de São Paulo (UNIFESP), 2018-06-28) Maiello, Fernando Pereira [UNIFESP]; Pesquero, Joao Bosco [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Many Differentiation Factors Play Important Roles In The Maturation Of Mesenchymal Osteoprogenitor To Osteoblast During The Skeletongenesis And Ossification Process, While One Of The Main Pathways In This Differentiation Via The Bone Morphogenetic Proteins. The Bmp Are Members Of Tgf-" Superfamily, A Class Of Similar Dimeric Proteins That Act Regularly As Local Mediator On The Regulation Of Many Biological Functions. The Use Of Bmp On Tissue Engineering And As Biopharmaceutical Drug Seems Very Useful And Its Production Is Described In The Literature. In This Work We Proposed To Clone, Express, Purify And Evaluate Recombinant Bmp4 Produced In Bacterial Systems. The Dna Sequence Of Bmp4 Was Obtained From Another Project Of The Group And Transferred To A Pqtev Plasmid. The Expression Was Evaluated In The Systems E. Coli. Bl21(De3), Rosetta (De3), Rosetta-Gami (De3) E Arctic (De3), Where It Was Produced Initially In The Insoluble Form And Solubilized During The Project. The Purification Was Performed From The Sol
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    Variação dos níveis de hemoglobina de pacientes em hemodiálise tratados com eritropoetina: uma experiência brasileira
    (Associação Médica Brasileira, 2010-01-01) Ammirati, Adriano Luiz [UNIFESP]; Watanabe, Renato [UNIFESP]; Aoqui, Cristiane [UNIFESP]; Draibe, Sergio Antonio [UNIFESP]; Carvalho, Aluizio Barbosa de [UNIFESP]; Abensur, Hugo; Drumond, Soraia Stael; Moreira, João; Bevilacqua, José Luis; Silva, Anita Cm; Tatsch, Fernando; Canziani, Maria Eugênia Fernandes [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP); Instituto de Nefrologia e Diálise; Instituto de Hemodiálise de Sorocaba; Roche Brazil
    OBJECTIVE: Correction of anemia using epoetin decreases morbidity and increases survival and quality of life in end-stage renal disease. Maintaining hemoglobin levels within the range proposed by guidelines has become a major challenge, with hemoglobin cycling affecting more than 90% of patients undergoing hemodialysis. The variability of hemoglobin levels over time was assessed in our patients. METHODS: Data were retrospectively collected on 249 patients undergoing hemodialysis over a 3-year period at seven centers in Brazil. Hemoglobin was measured at least monthly, and target levels were those between 10.5 g/dL and 12.5 g/dL. Patients were grouped into six categories of variability consistently low (<10.5g/dL), consistently target range (10.5 to 12.5 g/dL), consistently high (>12.5g/dL), low amplitude fluctuation with low hemoglobin levels, low amplitude fluctuation with high hemoglobin levels and high amplitude fluctuation. None of the patients maintained stable hemoglobin levels for the entire 36-month period. RESULTS: The mean monthly proportion of patients that had hemoglobin levels within the target range was 50% (range, 42% to 61%). Mean levels above the target (30%) were more frequent than those below it (20%). During 6, 12, and 36 months, proportions of patients with consistently low levels of hemoglobin decreased from 3.6% to 0%, from 31.7% to 2.8% for those with consistently high, from 7.6% to 0% for those with low amplitude fluctuation with low hemoglobin levels and from 41.3% to 8.3% for those with low amplitude fluctuation with high hemoglobin levels. However, the proportions of patients with high amplitude fluctuation increased from 21.5% to 88.9%. CONCLUSION: Maintaining hemoglobin levels within the target range is difficult, especially for longer periods of time. Missing the target seems more often due to levels above it, but high-amplitude fluctuations eventually occur in the majority of patients.