Navegando por Palavras-chave "Ratos Sprague-Dawley"

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    Avaliacao do transplante de celulas precursoras neurais no modelo experimental de epilepsia por pilocarpina
    (Universidade Federal de São Paulo (UNIFESP), 2012) Romariz, Simone Amaro Alves [UNIFESP]
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    Efeito da suplementação de ácidos graxos trans ou oligofrutose, durante a gestação e lactação, nas mães e prole com 90 dias de vida sobre o metabolismo e parâmetros inflamatórios
    (Universidade Federal de São Paulo (UNIFESP), 2016-04-30) Oliveira, Juliana Lopez de [UNIFESP]; Nascimento, Claudia Maria da Penha Oller do [UNIFESP]; Pisani, Luciana Pellegrini [UNIFESP]; Oyama, Lila Missae [UNIFESP]; http://lattes.cnpq.br/3983527783636073; http://lattes.cnpq.br/7125541171554727; http://lattes.cnpq.br/9974551337615485; http://lattes.cnpq.br/3558119106282470; Universidade Federal de São Paulo (UNIFESP)
    Introduction. Adverse effects of the consumption of trans fatty acids (TFA) have been widely studied by the scientific community in the last. Previously, our group found that the intake of trans fatty acids (TFA) during pregnancy and lactation promotes a pro-inflammatory state in the offspring at twenty-one and ninety days old. Moreover, literature data indicates that the prebiotic intake may alter the intestinal environment, presenting antiinflammatory properties. To evaluate the effect of a diet supplemented with OF (10%) or a diet rich in TFA (7%) during gestation and lactation on metabolic parameters and inflammatory lactating rats and ninety days old offspring, concerning endotoxemia, metabolic and inflammatory parameters. In addition, to assess whether the diet supplemented with OF (10%) modifies these parameters in the offspring, ninety days old offspring, of mothers who received diet containing TFA during pregnancy and lactation. Methods. On the first day of pregnancy, the rats were divided into three groups: control diet (C) control diet supplemented with 10% oligofructose (OF) and diet enriched with hydrogenated fat rich in TFA (T). The diets were maintained during pregnancy and lactation. In the twenty-first day of lactation animals were weaned and mothers euthanized. The pups were kept in individual cages within ninety days of life and than euthanazied. The blood, bowel, retroperitoneal adipose tissue (RET), parametrial fat mothers and epididymal of puppies, liver, soleus muscle tissues and EDL were collected. The serum was used for measuring lipopolysaccharide (LPS), free fatty acids (FFA), TNF- ?, hormones, insulin, ghrelin, adiponectin and NPY as well as cytokines by specific kits. The liver, and soleus and EDL RET muscle tissue were used for determination of cytokine content by specific kits. The liver and soleus muscle tissue were placed in a specific buffer to perform Western blotting. Genomic DNA Bacteria present in the colon feces was determined by real time PCR. Results. Supplementation with 10% of oligofructose during pregnancy and lactation reduced body weight gain, maternal dietary intake and serum adiponectin levels. In ninety days old offspring, maternal supplementation of oligofructose (10%) promoted increased IL-6 content and protein expression of IL-6R, and reduced protein expression of ADIPO-R1 in the soleus muscle. Maternal intake of TFA increased Lee index and the protein expression of pp65- NFkB in offspring with 90 days of life. Supplementation of offspring with
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    Vaporizador artesanal de éter para cirurgia experimental em pequenos roedores
    (Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 1998-01-01) Brito, Marcus Vinicius Henriques [UNIFESP]; Botelho, Nara Macedo [UNIFESP]; Almeida, Adriana de Jesus Benevides de; Santos, Marcos Roberto Lima De Carvalho; Universidade Federal de São Paulo (UNIFESP)
    This paper described a homemade ether vaporizer, changed from WAYNFORTH & FLECKNELL model for small animals. The device is build with hospital material and products bought in aquarium s shops. Their advantages are maintenance of anesthetic level without successive inductions by inhalatory nasal ether ; ether consumption of 5 ml per hour in animals with 300 g ; smaller ambient pollution and small cost. The disadvantages are a hardness and reduction of the plastic tubes after approximately 150 hour in use, by ether action and the necessity of experience acquisition in anesthetic manipulation with the device