Navegando por Palavras-chave "RUNX-2"

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    Características do tecido ósseo e cartilagíneo do fêmur de um modelo experimental para Distrofia Muscular de Duchenne
    (Universidade Federal de São Paulo (UNIFESP), 2019-07-08) Santos, José Fontes dos [UNIFESP]; De Oliveira, Flavia [UNIFESP]; http://lattes.cnpq.br/3387760393535776; http://lattes.cnpq.br/4452664613699869; Universidade Federal de São Paulo (UNIFESP)
    Duchenne muscular dystrophy (DMD) is induced by genetic mutation in the protein dystrophy. Patients suffering DMD have muscle weakness, loss of mobility and, greater bone resorption, reduced bone mass and greater susceptibility to fracture. The experimental MDX murine model, as in dystrophic humans, has a genetic mutation that causes the lack of dystrophin. However, unlike dystrophic humans, these animals have muscle regeneration and non-progressive disease phenotype. Thus, the study aims to evaluate bone and cartilage from femur of murine models DMD, C57BL/10-dmdmdx (MDX group, n=3), and their respective controls (Control group, n=3). Both animal groups were euthanized at 16 weeks-old. After that, the proximal epiphysis was separated and processed through histological techniques for obtaining some tissue that were subsequently submitted to staining HE and Sirius red staining for structural, cellular analysis such as, collagen and immunohistochemistry for RUNX-2/RANK-L; MMP-2 e MMP-9; Caspase-3 and KI-67. Statistical analyzes were performed by the unpaired Student t test. The results showed that both articular cartilage and bone tissue underwent severe morphological changes due to muscular dystrophy. In addition, histopathology analysis showed a predominance of type I collagen fibers, with the pantographic mesh compacted in the articular cartilage from dystrophic animals. In the immunohistochemical analyzes, the MDX group showed expression for all proteins used in this study, particularly in the regions of subchondral bone, medulla and diaphysis. These results indicate that the changes present in bone and cartilage tissue are not exclusively related to muscle weakness, but also due to action of the osteogenic regulation proteins and remodeling bone process.