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- ItemAcesso aberto (Open Access)Avaliação dos Métodos Empregados no Programa Nacional de Combate ao Câncer do Colo Uterino do Ministério da Saúde(Federação Brasileira das Sociedades de Ginecologia e Obstetrícia, 2001-05-01) Roberto Neto, Alfredo [UNIFESP]; Ribalta, Julisa Chamorro Lascasas [UNIFESP]; Focchi, José [UNIFESP]; Baracat, Edmund Chada [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Purpose: to evaluate a populational sample of the screening proposed by the National Program of Uterine Cervical Cancer Control (PNCC), regarding the following issues: frequency of unsatisfactory cytologic results, cytologic frequency of atypical squamous or glandular cells of undetermined significance (ASCUS, AGCUS), low- or high-grade squamous intraepithelial lesions (SIL), comparing the cytologic results with anatomicopathological results of colposcopically directed biopsies. Methods: through the written, broadcasting television and oral midia, women between 35 and 49 years were requested to have a preventive cytopathological test, to be collected by the authorized public health or other institutions accredited by SUS. The slides were analyzed by the program-authorized laboratories and all those patients from the populational sample from the municipality of Naviraí in the State of Mato Grosso do Sul with cellular alterations were submitted to colposcopy and directed biopsy. Results: the frequency of cytologic alterations of the ASCUS, AGCUS and SIL types was 3.3%, an index that is close to that predicted by the PNCC (4%); the percentage of samples that were unsatisfactory for evaluation was high (12.5%); among the ASCUS, AGCUS or low grade-SIL patients, 27.3% presented intraepithelial lesions of a high grade in the anatomicopathological study; while patients with cytology compatible with high grade-SIL, the directed biopsy revealed that 12.5% presented low-grade intraepithelial lesions. Conclusions: the choice of oncological cytology as the only method for the screening in the program allowed high indexes of false-negatives (27.3%) and of false-positives (12.5%). In the screening of cervical neoplasms, colposcopy has shown to be an important and indispensable method to guide the therapeutical management to be adopted.
- ItemSomente MetadadadoshTERT and TP53 deregulation in intestinal-type gastric carcinogenesis in non-human primates(Springer, 2013-08-01) Leal, Mariana Ferreira [UNIFESP]; Calcagno, Danielle Queiroz [UNIFESP]; Khayat, Andre Salim; Raiol Silva, Tanielly Cristina; Pereira Carneiro Muniz, Jose Augusto; Assumpcao, Paulo Pimentel; Cardoso Smith, Marilia de Arruda [UNIFESP]; Burbano, Rommel Rodriguez; Universidade Federal de São Paulo (UNIFESP); Fed Univ Para; Minist SaudeDespite the high incidence, the molecular events involved in intestinal-type gastric carcinogenesis remains unclear. We previously established an intestinal-type gastric carcinogenesis model in Cebus apella, a New World monkey. in the present study, we evaluated hTERT and TP53 mRNA expression, as well as their protein immunoreactivity, in normal mucosa, non-atrophic gastritis, atrophic gastritis, intestinal metaplasia, and intestinal-type gastric cancer samples of non-human primates treated with N-methyl-nitrosourea. in addition, we evaluated the number of TP53 copies in these samples. Although hTERT immunoreactivity was only detected in gastric cancer, a continuous increase of hTERT mRNA expression was observed from non-atrophic gastritis to gastric tumors. No sample presented p53 immunoreactivity. However, we also observed a continuous decrease of TP53 mRNA expression during the sequential steps of gastric carcinogenesis. Moreover, loss of TP53 copies was observed in intestinal metaplasia and gastric cancer samples. Our study highlights that hTERT and TP53 have a key role in intestinal-type gastric cancer initiation.
- ItemAcesso aberto (Open Access)hTERT, MYC and TP53 deregulation in gastric preneoplastic lesions(Biomed Central Ltd, 2012-07-06) Silva, Tanielly Cristina Raiol; Leal, Mariana Ferreira [UNIFESP]; Calcagno, Danielle Queiroz [UNIFESP]; Souza, Carolina Rosal Teixeira de; Khayat, Andre Salim; Santos, Ney Pereira Carneiro dos; Montenegro, Raquel Carvalho; Rabenhorst, Silvia Helena Barem; Nascimento, Mayara Quaresma; Assumpcao, Paulo Pimentel; Smith, Marilia de Arruda Cardoso [UNIFESP]; Burbano, Rommel Rodriguez; Universidade Federal de São Paulo (UNIFESP); Fed Univ Para; Univ Fed CearaBackground: Gastric cancer is a serious public health problem in Northern Brazil and in the world due to its high incidence and mortality. Despite the severity of the disease, more research is needed to better understand the molecular events involved in this intestinal-type gastric carcinogenesis process. Since precancerous lesions precede intestinal-type gastric cancer, here, we evaluated the hTERT, MYC, and TP53 mRNA and protein expression, as well as TP33 copy number, in gastric preneoplastic lesions.Methods: We evaluated 19 superficial gastritis, 18 atrophic gastritis, and 18 intestinal metaplasia from cancer-free individuals of Northern Brazil. Quantitative reverse transcription PCR was used to analyze the mRNA expression and immunohistochemical methods were used to assess protein immunoreactivity in tissue samples. the number of TP53 gene copies was investigated in gastric diseases by quantitative PCR.Results: We observed hTERT, MYC, and p53 immunoreactivity only in intestinal metaplasia samples. the immunoreactivity of these proteins was strongly associated with each other. A significantly higher MYC mRNA expression was observed in intestinal metaplasia compared to gastritis samples. Loss of TP53 was also only detected in intestinal metaplasia specimens.Conclusions: We demonstrated that hTERT, MYC, and TP53 are deregulated in intestinal metaplasia of individuals from Northern Brazil and these alterations may facilitate tumor initiation.