Navegando por Palavras-chave "Photoaging"

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    Comparação das medidas instrumentais não invasivas e parâmetros clínicos entre o envelhecimento intrínseco e o fotoenvelhecimento da pele dos antebraços
    (Universidade Federal de São Paulo (UNIFESP), 2017-04-28) Carvalho, Paula Roberta Silva de [UNIFESP]; Bagatin, Edileia [UNIFESP]; http://lattes.cnpq.br/6478900066830476; http://lattes.cnpq.br/5342802165966201; Universidade Federal de São Paulo (UNIFESP)
    Background: The intrinsic and extrinsic skin aging present biomechanical and morphological differences, which are reflected in the appearance of roughness, superficial and deep wrinkles, atrophy, reduced elasticity, hypo- and hyperpigmentation and actinic keratosis. The photodamage causes accentuation of all these features and increases the risk for development of premalignant lesions (actinic keratosis) and malignant (basal and squamous cell carcinomas). Objective: to evaluate and compare the characteristics of the flexor (with a predominance of intrinsic aging) and extensor (chronically exposed to sunlight area and other environmental factors, with a predominance of extrinsic aging or photoaging) skin surfaces of the forearms. Methods: The cross-sectional study of diagnostic interventions, including 23 females, aged over 60 years. The extensor and flexor faces of right and left forearms were compared in relation to clinical parameters and non-invasive instrumental measures, such as: skin surface (profilometry), viscoelastic properties, hydration (corneometry) as well as dermal thickness and echogenicity (ultrasound). Results: The water content in the corneal layer showed a significant difference as the flexor area showed an average value greater than the extensor face. The average of the measurements obtained by high-frequency ultrasound showed difference between the faces. The skin surface measurements showed significant differences when compared roughness and desquamation of the extensor and flexor faces on both forearms, but for the flexor face, the differences were smaller. Regarding the wrinkles depth, there was a significant difference of average between the evaluated faces only for the left forearm (p = 0.017), once the flexor face presented an average value greater than the extensor. There was no significant correlation between measurements of the aqueous content of the corneal layer and the viscoelastic properties of the skin, as well as among those obtained by ultrasound. In the prophylometry, only the scaling presented a negative correlation with the corneometry. The visco-elastic properties of the skin showed a positive correlation between the faces. The measurements obtained by the ultrasound and the visco-elastic properties of the skin showed a positive correlation in the flexor face. In the extensor face, there was a positive correlation between roughness, scaliness and wrinkle depth. In the flexor face, the depth of the wrinkles presented negative correlation with a dermal thickness. Dermal density showed negative correlation with other ultrasound measurements. These results were in agreement with established clinical parameters such as wrinkles, elastosis, decreased elasticity and water content, demonstrating the differences between photoaging and chronological aging. Conclusion: Significant differences in the biophysical characteristics of the extensor and flexor surfaces of the forearms were detected. The non-invasive instrumental measures were positively correlated with clinical findings. We concluded that they are useful tools for the evaluation of photoaging and chronological aging in clinical research, as efficacy and safety parameters.
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    In vivo confocal Raman spectroscopy for intrinsic aging and photoaging assessment
    (Elsevier Ireland Ltd, 2017) Nasser Caetano, Livia de Vasconcelos [UNIFESP]; Mendes, Thiago de Oliveira; Bagatin, Edileia [UNIFESP]; Miot, Helio Amante; Marques Soares, Juliana Laudiceia [UNIFESP]; Enokihara, Milvia Maria Simoes e Silva [UNIFESP]; Martin, Airton Abrahao
    Background: In vivo confocal Raman spectroscopy is a non-invasive method to assess either the epidermis or the dermis composition. Few studies have focused on dermis collagen alterations through intrinsic aging and photoaging. Objective: This study evaluated the in vivo Raman spectra from the dermis of a photoexposed site versus a non-photoexposed region in different age groups, and evaluated the correlation between peak intensities and age, photoaging score and the amount of collagen assessed with histology and high frequency ultrasound (HFUS). Methods: Fifteen volunteers aged 28-82 years were divided into three groups according to forearm photoaging degree. In vivo Raman spectra from the dermis were collected on the dorsal forearm (chronically photoexposed skin) and on the proximal medial arm (non-photoexposed skin). Crosssectional images of the skin were obtained using a 20 MHz ultrasound unit exactly on the same sites, which were further submitted to punch biopsies for histologic study (collagen I immunohistochemistry, picrosirius red staining and Verhoeff). Principal Component Analysis (PCA) and Orthogonal Partial Least Squares Discriminant Analysis (OPLS-DA) were taken in the spectral region of 796 cm(-1) -996 cm(-1) to determine its potential to discriminate between different groups. The Spearman rank correlation coefficient of individual peak intensities and ratios with age, clinical score and the amount of collagen assessed by ultrasound and histology were calculated. Results: PCA of pairs of groups and OPLS-DA could discriminate the intrinsically from the photoaged skin and the young group from the elderly one, with important contribution of the 938 cm(-1) and 855 cm(-1) peaks intensities. The intensity of the peaks in 855 cm(-1) and/or 938 cm(-1) presented moderate correlation with age (rho = 0.579, p = 0.049) and moderate to high inverse correlation with HFUS echogenicity (rho = -0.710, p = 0.010) and collagen I immunohistochemistry (rho = -0.833, p = 0.005) in the non-photoexposed region. The I-1275/I-1450 intensities ratio presented moderate to high correlation coefficients with age (rho = -0.730, p = 0.007), photoaging score (rho = -0.594, p = 0.042), HFUS echogenicity (rho = 0.760, p < 0.001) and histology (collagen I immunohistochemistry (rho = 0.643, p = 0.024), picrosirius (rho = 0.773, p = 0.005) and Verhoeff (rho = -0.727, p = 0.011)) in the photoexposed site. Conclusion: The wavenumber region between 798 and 994 cm(-1) is useful for the analysis of dermal collagen alterations through the intrinsic aging process, while photoaging is better assessed by the I-1275/I-1450 intensities ratio. This is the first skin aging study to show a correlation between Raman peaks and the amount of collagen assessed by HFUS- and histology. (C) 2017 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.