Navegando por Palavras-chave "Phenois"

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    Obtenção e estudo fitoquímico do extrato hidroglicólico de Mouriri Elliptica Martius (Melastomataceae), aplicação em formulação fotoprotetora e avaliação in vitro do FPS
    (Universidade Federal de São Paulo, 2018-12-07) Ramos, Ana Carolina Massarani [UNIFESP]; Andreo, Marcio Adriano [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Nowadays life expactancy is increasing and with it the skin ages. A loss of function and differences in skin appearance proceed unavoidably. Exposure to UV radiation can induce cellular changes in skin cells, resulting in a set of signals called photoaging. In this context, sunscreens act in a preventive way and the development of products with natural actives could help. Many plant extracts present antioxidant and photoprotective activity that is generally linked to the presence of phenolic substances produced by plants. In the species Mouriri elliptica Martius (Melastomataceae), whose leaves are traditionally used for problems of the digestive tract, phenolic substances (tannins and flavonoids) have been identified that have a direct relation with the antioxidant capacity verified in the hydroalcoholic extracts of this species. The objective of this study was to evaluate the photoprotective capacity (SPF) and photostability of formulations developed with the addition of the standardized hydroglicholic extract prepared from the leaves of M. elliptica (Melastomataceae). Spectrophotometric (UV-Vis) and chromatographic techniques (HPLC-DAD) were applied to qualitatively and quantitatively determine the markers. By DPPH the antioxidant activity of the extract after chemical standardization was evaluated. Furthermore the stability of formulations containing the extract was performed. Besides analysis of the formulations’ photostabilization, the Sun Protection Factor (SPF) of the main formulations was examined in vitro. By chemical analyzes it was possible to develop a standardized extract in: 1,91 mg/mL of total phenols, 1,19 mg / mL of total tannins, 0,26 mg / mL of total flavonoids and 48,7 mg / mL of gallic acid. After stability study, the formulations developed with the addition of 3%, 5% and 10% of this extract were completely stable. However, for the sun protection factor and photostability tests, the formulations showed no activity.