Navegando por Palavras-chave "Palatable High-Fat Diet"

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    Efeito de uma dieta obesogênica, do treinamento aeróbio e dos níveis de estradiol no estado redox do músculo esquelético de ratas Wistar
    (Universidade Federal de São Paulo (UNIFESP), 2020-05-21) Santos, David Pedro Dos [UNIFESP]; Lambertucci, Rafael Herling [UNIFESP]; Universidade Federal de São Paulo
    Introduction: Obesity can lead to adipose tissue remodeling, changing its structure and composition. This phenomenon generates a systemic pro-inflammatory state associated with oxidative stress, which leads to muscle damage and metabolic dysfunctions. Physical activity positively modulates the antioxidant system against such disturbances. Evidence shows that estradiol, due to its antioxidant action, can protect the body from such aggressions. Objective: Evaluate how estradiol levels can modulate skeletal muscle’s redox state of Wistar rats submitted to a high fat diet and aerobic training. Materials and Methods: 40 female adult Wistar rats were used. The estrous cycle was monitored by analyzing vaginal smears. Among 80 rats, which were divided by quartiles considering the observed estradiol concentration, we used the animals that were in the first (Q1) and third (Q3) quartiles, thus totaling 40 rats. After the adaptation period, the rats were divided into 4 groups (n = 10) according to the diet offered and the performance or not of exercises, which: CS - received a control diet and remained sedentary; CT - received a control diet and performed physical training; HS - received a palatable high-fat diet and remained sedentary; HT - received a palatable high-fat diet and underwent physical training. We performed statistical analysis using the Generalized Linear Model and Fisher's post-test. Results: With the exception of the sedentary group fed with control diet, all groups with high estradiol showed higher total antioxidant status (TAS), at the same time, the groups with high estradiol had higher values of total oxidative status (TOS). The sedentary group with a high-fat diet and high estradiol had a lower oxidative stress index (OSI), compared to the trained group with a high-fat diet and low estradiol. Other than the sedentary group with a high-fat diet, all groups with high estradiol had higher values for the determination of lipid peroxidation (TBARS). The groups with high estradiol had a higher concentration of carbonylated protein. Except for the sedentary group with a control diet, all other groups with high estradiol showed higher nitric oxide values. Sedentary animals with high estradiol fed a high-fat diet had a higher catalase enzimatic activity when compared to animals with low estradiol Regarding the superoxide dismutase enzyme (SOD), all variables showed statistically significant difference, being: estradiol levels (χ2 = 21.346, p <0.001), training (χ2 = 21.004, p <0.001) and diet (χ2 = 4.644, p = 0.031), in addition to the interaction between estradiol levels with training (χ2 = 11.904, p <0.001), and estradiol levels with diet (χ2 = 8.668, p <0.003). For the group trained with a high-fat diet and a high level of estradiol, the value of glutathione peroxidase (GPX) activity was higher. Conclusion: Estradiol levels can interfere with the oxidative stress modulatory process and physical training can control it and improve the antioxidant profile. Regardless of estradiol levels, we observed that the isolated high-fat diet did not induce oxidative stress.