Navegando por Palavras-chave "Ovos De Galinha"

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    Bioprospecção de Novos Peptídeos Antimicrobianos em Ovos de Galinha Gallus gallus domesticus
    (Universidade Federal de São Paulo (UNIFESP), 2019-07-25) Santos, Sandra Regina Dos [UNIFESP]; Miranda, Antonio De [UNIFESP]; Silva Jr, Pedro Ismael da [UNIFESP]; http://lattes.cnpq.br/7158586957386749; http://lattes.cnpq.br/1357848049935882; http://lattes.cnpq.br/6321614296807612; Universidade Federal de São Paulo (UNIFESP)
    A long time the eggs avian developed some defense systems for minimize the predatory animals, for example, desenvolviment a rigid limestone strutucture (shell). However for to success of the embryo the presence of molecular components there are related with nutrition of the embryo as the may also be associated with capacity to inhibit the growth and proliferation of the microorganisms present in the environment in where these eggs live. So, can these components have some biotechnological application or therapeutic, mainly focusing on the use against microorganisms with showed mechanisms of resistance to conventional antibiotics? This study aimed to evaluate the presence of bioactive molecules, focusing in antimicrobial peptides, in the extract of chicken eggs of the species Gallus gallus domesticus. For this was used the extract of chicken eggs without the shel of the specie Gallus gallus domesticus, after the steps of fractionation several fractions were obtained with antimicrobial potential against Micrococcus luteus A270 (bacterial Gram-positive) and Eschericchia coli D31 (bacterial Gram-negative), Aspergillus niger (filamentous fungus – isolated of the environment), Candida albicans MDM8 and Cryptococcus neoformans strain99 (yeasts). Two fractions obtained of the elution 80% acetonitrile were characterized, sequenced and submeted the solid phase peptide synthesis using strategy t-Boc. The peptide Ovoin, with sequence SVSTVLTSKYR and molecular weight 1240 Da was active against M.luteus and C. neoformans, but this sequence did not similarity in searches made database, could be a new antimicrobial peptide present in chicken eggs. And the peptide Ovipin, with sequence GYSFTTTAER and molecular weight 1131 D, was active against M. luteus, E. coli, A. niger e C. neoformans. The searches made in database was demonstrated that this sequence is a fragment of actin protein of the Gallus gallus domesticus.