Navegando por Palavras-chave "Nasal Polyps"

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    CD 133, marcador de células progenitoras na polipose nasal e suas relações com IL-12p40, IL12p70 e Il-8
    (Universidade Federal de São Paulo (UNIFESP), 2020-01-30) Lino, Wagner Vargas Souza [UNIFESP]; Pezato, Rogerio [UNIFESP]; Universidade Federal de São Paulo
    Introduction: Nasal Polyposis is à disease based on two pillars: chronic inflammatory process and altered remodeling process that leads to mechanical dysfunction. The stem cells have the potential to mitigate the inflammatory process and tissue regeneration. The PN derived stem cells shows gene expression related to protein grant increased when compared to healthy nasal mucosa, such as CD133, increased the healthy nasal mucosa. CD133 is a marker of progenitor cells, associated with stem cells and tissue growth and neoplasia. Therefore, this led us to investigate the concentration of CD133 in polypoid tissue and its relationships with major inflammatory interleukins. Objective: To evaluate CD133 protein expression in nasal polypoid tissue and its relationship with IL-12P40, IL-12p70 and IL-8. Method: The sample consisted of 74 participants, divided into two groups: one of patients with nasal polyposis (n = 39) according to EPOS 2012 criteria and a control group without inflammatory nasal mucosa disease (n = 35). It was measured in nasal polyp tissue in the Nasal Polyposis group and in the middle meatus mucosa of the control group: TGF-β by multiplex and IL-8, IL-12p40 and IL-12p70, CD133 by the Elisa method. The presence of eosinophils was evaluated by immunohistochemistry. Results: The eosinophil count in the Nasal Polyp sample was above 5 eosinophils per High-Power Field in 91% and in 73% of the cases presented more than 10 eosinophils per HighPower Field. CD133 and TGF-β levels were significantly lower in the polypoid tissue group when compared to healthy nasal tissues from middle meatus. Dosage of interleukins IL-8, IL-12p40 and IL-12p70 were significantly lower in the group with nasal polyps when compared to the control group. There was a significant positive correlation between CD133 and IL-8, IL-12p40, IL-12p70 in the control patients, and this correlation was weakened in patients with nasal polyposis and especially in aspirin intolerant patients. There was no correlation between CD133 and TGF-β regardless of which group compared. Conclusion: CD133 and IL-8, IL-12p40 and IL12p70 are decreased in nasal polyps tissue compared to normal mucosa, also in nasal polyposis there was weakening of positive correlations found between CD133 and IL-12. 8, IL-12p40 and IL-12p70 in normal mucosal tissue. These findings demonstrate the difficulty of nasal polypoid tissue to adequately respond to the increase in inflammatory interleukins through its progenitor cells, since this tissue does not have a predictable response to related Th interleukins.
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    Expressão proteica de BMP-7, MMP-9 e TGF-β e suas correlações com as interleucinas 6 e 10 nas rinossinusites crônicas
    (Universidade Federal de São Paulo (UNIFESP), 2020-08-27) Lucas, Bernardo Relvas [UNIFESP]; Pezato, Rogerio [UNIFESP]; Universidade Federal de São Paulo
    Introduction: Chronic Rhinosinusitis has a worldwide prevalence in the general population of 5% to 12% and is divided phenotypically into two main groups: Chronic Rhinosinusitis without Nasal Polyposis and Chronic Rhinosinusitis with Nasal Polyposis, presenting processes distinct remodeling. Involved in the remodeling process, the BMP-7, MMP-9 and TGF-β proteins appear to exhibit different behaviors in the two phenotypes and different relationships with the interleukins IL-6 and IL-10, which will be investigated in this study. Objective: to evaluate the expression of BMP-7, MMP-9, TGF-β protein in Rhinosinusitis with and without Nasal Polyposis and its relationship with interleukins IL-6 and IL-10. Method: the sample consisted of 86 participants, divided into three groups: one of patients with Chronic Rhinosinusitis With Nasal Polyposis (n = 34), one of patients with Chronic Rhinosinusitis without Nasal Polyposis (n= 26), both groups according to EPOS 2012 criteria, and a control group without inflammatory nasal mucosa disease (n = 26). BMP-7, MMP-9, TGF-β, IL-6 and IL-10 were dosed into the tissue by Elisa method. The presence of eosinophils was evaluated by immunohistochemical Hematoxylin/ Eosin. Results: the high-power field (HPF) eosinophil count in the Nasal Polyp sample was above 5 eosinophils per high-power field in 91% of the cases and 73% presented more than 10 eosinophils by High-Power Field. TGF-β level were significantly lower in the polypoid tissue group when compared to control group and Chronic Rhinosinusitis without Nasal Polyposis group. Dosage of IL-6 was significantly higher in the Nasal Polyps group when compared to the other groups. Dosage of IL-10 was significantly higher in control group when compared to the other groups. There were significant negative correlations between IL-6 and IL-10 in all group, and between MMP-9 and IL-6 in control group and Nasal Polyp group. There was a positive correlation between MMP-9 and IL-10 in Nasal Polyp group. There was a positive correlation between BMP-7 and IL-10 in all groups. Finally, the correlation between TGF-β and IL-6 was positive in control group and negative in Chronic Rhinosinusitis without Nasal Polyposis group. Conclusion: In Chronic Rhinosinusitis without Nasal Polyposis there is a low concentration of IL-10 and a high concentration of TGF-β. In Nasal Polyposis there is a low concentration of TGF-β and IL-10 and an increase in IL-6. IL-6 and IL-10 have a negative correlation with each other and distinct interactions with BMP-7 and MMP-9 in the groups analyzed. We concluded, therefore, that there is a close relationship between interleukins 6 and 10 and the main proteins involved in the remodeling process in Chronic Rhinosinusitis.
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    Propriedades angiogênicas do pólipo nasal no modelo experimental da membrana corioalantóica de embrião de galinha
    (Universidade Federal de São Paulo (UNIFESP), 2019-12-18) Goes, Hallyson Andre Nascimento De [UNIFESP]; Pezato, Rogerio [UNIFESP]; Gomes, Lígia Ferreira [UNIFESP]; http://lattes.cnpq.br/4950570091832315; http://lattes.cnpq.br/8850675385685321; http://lattes.cnpq.br/5391537042617179; Universidade Federal de São Paulo (UNIFESP)
    Introduction: Nasal polyps are benign, semi-translucent, edematous masses that develop with anomalous growth from the mucosa lining the paranasal cavity, usually originating from the middle meatus mucosa. The participation of angiogenesis in nasal polyposis is fundamental for tissue growth, being proposed based on the positive regulation of proangiogenic factors in polyps, resulting from inflammation, edema or epithelial rupture. Objective: To investigate the potential of nasal polyp tissue to promote or modify angiogenesis. Methods: The effects of the implantation of 12 middle turbinate polyp fragments and 10 intact middle meatus nasal mucosa tissue fragments on the embryo chorioallantoic membrane were studied. The effects on embryonic development were described and correlated with the observed effects on chorioallantoic membrane vessel angiogenesis in 57 embryonic eggs divided into three experimental groups according to the type of tissue implanted: “control”; “Mucosa” and “polyp”. Angiogenesis was assessed by digitized membrane images, by the area and branching index of the medium and large vessels. Embryonic development was evaluated by weight, length and stage according to Hamilton and Hamburger. Results: The implanted tissues differentially modified membrane angiogenesis and embryo development. Nasal polyps, but not mucosal tissues, demonstrated the potential to promote or modify angiogenesis in the embryonated egg of Gallus Domesticus during the studied period (E6-E8). Polyp tissue implants were compatible with the normal development of Gallus Domesticus; mucosal implants induced delays in embryo development. Conclusion: Nasal polyp tissue has a higher angiogenic capacity than normal nasal mucosa tissue, favoring the nutrition and development of polypoid tissue.