Navegando por Palavras-chave "Monocamada lipídica"

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    Estudo da interação do gama-terpineol em filmes de Langmuir como modelos de membrana
    (Universidade Federal de São Paulo, 2021-01-29) Jaroque, Guilherme Nuñez [UNIFESP]; Caseli, Luciano [UNIFESP]; Sartorelli, Patricia [UNIFESP]; http://lattes.cnpq.br/6836392358779448; http://lattes.cnpq.br/8929162910172931; http://lattes.cnpq.br/3306052344968821
    This work aimed to study the interaction of γ-terpineol with lipid Langmuir monolayers, which served as a model for cell membranes to analyze the possible biological actions of the compound at the molecular level. The phospholipids used to compose the films were dipalmitoylphosphatidylcholine (DPPC), dipalmitoyl phosphatidylethanolamine (DPPE), pure or mixed with cholesterol or with bacterial peptidoglycan. The lipids were spread on the air-water interface through organic solutions assembling a Langmuir monolayer. The γ-terpineol compound was incorporated in the monolayer by co-spreading it with the lipids. The interfacial films formed at the air-water interface were compressed by movable barriers, reaching surface pressures that approached the viscoelastic and thermodynamic properties of a proteolipid bilayer. The interaction of the drug with the monolayers was analyzed using tensiometric measurements (surface pressure- area isotherms, tensiometric stability, hysteresis assays), Brewster angle microscopy, and polarization-modulation infrared reflection-adsorption spectroscopy. The physicochemical properties obtained by these measurements were analyzed, and the interactions of γ-terpineol with the lipids at the air-water interface were inferred and extrapolated for the action of the compound in related cell membranes.