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- ItemSomente MetadadadosProspecção biotecnológica de microrganismos marinhos com potencial anticâncer recuperados de sedimento do arquipélago de alcatrazes e sua utilização como suporte à conservação(Universidade Federal de São Paulo, 2018-09-25) Falcão, Gabriela Ciscotto [UNIFESP]; Jimenez, Paula Christine [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)The Alcatrazes Archipelago (AA), after nearly 30 years of discussions, and based on research that demonstrated its ecological relevance, the presence of endemic species that were endangered and also some rare species, became a Federal Conservation Unit (CU), declared a Wildlife Refuge in August 2016. In this context, and with the growing discoveries of genes and molecules from marine species potentially endowed with therapeutic value, such as drugs, the goal of the present study was to evaluate the biotechnological patrimony housed in microorganisms recovered from the AA sediment based on the evaluation of the cytotoxic potential of their organic extracts in a model of tumor cells in culture, metabolomic analysis of these extracts and molecular identification of the strains considered most relevant. A total of 39 strains of bacteria were recovered from the sediment and, of these, 17 produced extracts that were active in the cytotoxicity tests. Through a metabolomic analysis by molecular network generated on the GNPS platform, it was possible to identify the occurrence of the molecules such as Surfactin, Colic Acid, Ferrioxamine and Desferrioxamine among the analyzed extracts, in addition to a large number of unidentified precursor ions. The 17 active strains underwent molecular identification at the genus level and a construction of a clustering tree, where it was possible to observe that among them there were bacteria of the genera Bacillus, Pseudomonas, Halomonas and Psychrobacter. There was a pattern in the presence of the molecules identified within the genus, indicated by clusters of precursor ions unique to extracts obtained from bacteria of the genus Bacillus, whereas other clusters were typical of Gram-negative bacteria and, therefore, present in the other genera elucidated. The results presented herein demonstrate the biotechnological potential housed in the AA. As a new CU, the sustainable exploration of the Alcatrazes Archipelago must have great relevance in the perspective of economically valuing species that do not have other measurable value, deriving new bioproducts with minimum impacts to the region and, thus, harness the conservation of this ecologically valuable marine area of Brazil to the provision of this important ecosystem service.