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- ItemAcesso aberto (Open Access)Análise metabolômica urinária de mulheres com cistite intersticial(Universidade Federal de São Paulo (UNIFESP), 2019-04-16) Silva, Camila Gonzaga Da [UNIFESP]; Almeida, Fernando Gonçalves de [UNIFESP] ; http://lattes.cnpq.br/9336208077764596; http://lattes.cnpq.br/5009618939627098; Universidade Federal de São Paulo (UNIFESP)Objectives: To compare urinary metabolites subsets of organic acids, acylcarnitines and amino acids, and creatinine among women with and without interstitial cystitis (IC)/Painful Bladder Syndrome (PBS). Methods: The medium flow of the first-morning urine from 20 women with IC and 20 healthy women (without urinary complaint) was collection and processed to obtain a sample filtrate. From this, the metabolites were extracted through protein precipitation method, followed by metabolites target analysis with Ultra-Performance Liquid Chromatography - Mass Spectrometry (UPLC-MS). Quantification of targeted metabolites was performed based on external calibrations curve of analytical standards and the data were evaluated by non-parametric statistical tests. Results: Urinary concentrations of malic acid, kynurenic acid, succinic acid, uric acid, creatinine and leucine differ for women with IC/PBS compared to women without urinary complaints, being a higher urinary uric acid concentration for symptomatic women. Conclusion: Women with IC/PBS have in urine a difference of metabolites correlated with energetic metabolism which may be candidates for biomarkers for this syndrome.