Navegando por Palavras-chave "Mesenteric adipose tissue"

Agora exibindo 1 - 2 de 2
  • Imagem de Miniatura
    Item
    Acesso aberto (Open Access)
    Alterações promovidas pela caquexia associada ao câncer sobre a inervação do tecido adiposo de pacientes caquéticos
    (Universidade Federal de São Paulo (UNIFESP), 2016-09-30) Cavalaro, Diego Alexandre [UNIFESP]; Nascimento, Claudia Maria da Penha Oller do [UNIFESP]; http://lattes.cnpq.br/9974551337615485; http://lattes.cnpq.br/4545663567537745; Universidade Federal de São Paulo (UNIFESP)
    Introduction: Cachexia is a multifactorial syndrome, characterized by loss of skeletal muscle tissue and / or adipose tissue, which cannot be completely reversed even with nutritional support, and is often associated with advanced stages of cancer. The sympathetic nervous system (SNS) may have a role in the development of this syndrome by controlling lipid metabolism through catecholamines. Objective: To analyze possible differences in sympathetic innervation, adrenergic receptors in the inflammatory process in the mesenteric adipose tissue of patients with cancer cachexia , non-cachectic and controls. Methods: The study involved 87 patients, recruited from the University Hospital of the University of São Paulo, classified into 3 groups: Control, cancer without cachexia (WSC) and cancer cachexia (CC). Blood samples were collected for biochemical measurements. Mesenteric adipose tissue samples employed used for determination of cytokines, gene expression, protein concentration, expression of adrenergic receptors and to analyze the frequency of adrenergic fiber immunoreactivity for anti-tyrosine hydroxylase. Results: CC showed greater weight loss, anemia, hypoalbuminemia, higher concentrations of C-reactive protein (CRP) and lower score ( p<0.0001 ) for quality of life. CC also showed higher plasma concentrations of interleukin-6 (IL-6) and tumor necrosis factor-? (TNF-?). Significant differences in gene expression and protein quantification of adrenergic receptors in mesenteric adipose tissue were not observed. The qualitative analysis of the density of adrenergic fiber in the mesenteric adipose tissue was not different between groups. Conclusions: CC patients showed a inflammatory profile presenting higher plasma concentrations of pro inflammatory cytokines and PCR. No changes were found for gene expression and protein quantification of adrenergic receptors in mesenteric adipose tissue of patients with cancer cachexia, nor weight- stable counterparts. Conclusion: The results suggest that the development of cachexia is independent of changes in the gene expression and protein quantification of alpha and beta adrenergic receptors, as well as changes in the presence of adrenergic fibers, in the mesenteric adipose tissue.
  • Imagem de Miniatura
    Item
    Acesso aberto (Open Access)
    Coacervate whey protein improves inflammatory milieu in mice fed with high-fat diet
    (Biomed Central Ltd, 2014-03-28) Moreno, Mayara Franzoi [UNIFESP]; Souza, Gabriel Inacio de Morais Honorato de [UNIFESP]; Hachul, Ana Claudia Losinskas [UNIFESP]; Santos, Bruno dos [UNIFESP]; Okuda, Marcos Hiromu [UNIFESP]; Pinto Neto, Nelson Inacio [UNIFESP]; Boldarine, Valter Tadeu [UNIFESP]; Esposito, Elisa [UNIFESP]; Ribeiro, Eliane Beraldi [UNIFESP]; Nascimento, Claudia Maria da Penha Oller do [UNIFESP]; Ganen, Aline de Piano [UNIFESP]; Oyama, Lila Missae [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Background: Functional foods with bioactive properties may help in treat obesity, as they can lead to a decreased risks of inflammatory diseases. the aim of this study was to investigate the effects of chitosan coacervate whey protein on the proinflammatory processes in mice fed with high-fat diet.Methods: Mice were divided into two groups receiving either a normolipidic or high-fat diet; the animals in each of the two diet groups were given a diet supplement of either coacervate (gavage, 36 mg protein/kg of body weight) or tap water for four weeks [groups: normolipidic diet plus water (C); normolipidic diet and coacervate (CC); high-fat diet and water (H); and high-fat diet and coacervate (HC)].Results: the high-fat diet promoted inflammation, possibly by decreased adiponectin/sum of adipose tissues ratio and increased phosphorylation of NF-kappa B p50. in HC we observed a positive correlation between IL-10 and TNF-alpha in mesenteric adipose tissue, retroperitoneal adipose tissue and liver tissue. We also observed a positive correlation between lipopolisaccharide with IL-10 in the liver tissue.Conclusions: High-fat diet treatment promoted metabolic alterations and inflammation, and chitosan coacervate whey protein modulated inflammatory milieu.