Navegando por Palavras-chave "Melanogenesis"

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    Anti-wrinkle and anti-whitening effects of juca (Libidibia ferrea Mart.) extracts
    (Springer, 2016) Pedrosa, Tatiana do Nascimento; Barros, Aline Oliveira; Nogueira, Jessica Rodrigues; Fruet, Andrea Costa; Rodrigues, Isis Costa; Calcagno, Danielle Queiroz [UNIFESP]; Smith, Marilia de Arruda Cardoso [UNIFESP]; de Souza, Tatiane Pereira; de Moraes Barros, Silvia Berlanga; de Vasconcellos, Marne Carvalho; Araujo da Silva, Felipe Moura; Ferreira Koolen, Hector Henrique; Maria-Engler, Silvya Stuchi; Lima, Emerson Silva
    Skin aging is a natural process of the human body that may be accelerated due to extrinsic causes. Libidibia ferrea, popularly known as juca, is a small tree, which possesses an abundant phenolic composition with potential antioxidant and enzymatic inhibition activities. Thus, this work aimed to investigate the anti-wrinkle and anti-whitening potentials of juca trunk bark (LFB) and pod (LFP) extracts. A comprehensive analysis of LFB and LFP phenolic composition was accomplished by means of liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Effects on skin degradation were assessed by inhibitory enzymatic activity against elastase, hyaluronidase and collagenase through colorimetric assays. Cellular viability in B16F10 and primary fibroblasts were determined by Trypan Blue exclusion assay. Anti-melanogenic effects on B16F10 cells were evaluated using cellular tyrosinase, melanin content, western blot, and RT-qPCR analyses. Inhibition of matrix metalloproteinase-2 and metalloproteinase-9 (MMP-2 and MMP-9) was determined by gelatin zymography and western blot methodologies. LC-MS/MS analyses of LFB and LFP extracts allowed the characterization of 18 compounds, among them, flavonoids, phenolic acids, and secoridoids. Additionally the pod and trunk bark compositions were compared. Hyaluronidase inhibitory activity for both extracts, LFB (IC50 = 8.5 +/- 0.8 A mu g/mL) and LFP (IC50 = 16 +/- 0.5 A mu g/mL), was stronger than standard rutin (IC50 = 27.6 +/- 0.06). Pro-MMP-2 was significantly inhibited by both extracts. LFB and LFP decreased the melanin content in B16F10 due to tyrosinase inhibitory activity. L. ferrea extracts has high potential as a cosmetic ingredient due to its anti-wrinkle and depigmentant effects.