Navegando por Palavras-chave "Lymph node biopsy"

Agora exibindo 1 - 2 de 2
  • Imagem de Miniatura
    Item
    Acesso aberto (Open Access)
    Experimental model in rat for sentinel node biopsy
    (Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2003-01-01) Oliveira Filho, Renato Santos de [UNIFESP]; Paiva, Geruza Rezende [UNIFESP]; Wagner, Jairo; Nogueira, Solange Amorim; Universidade Federal de São Paulo (UNIFESP); Hospital Israelita Albert Einstein Nuclear Medicine Service
    Although sentinel node procedure has been used world wide, there are many aspects to be defined and better standardized. This study address if the experimental model in rats is appropriate for sentinel node biopsy. In this model, the lymph nodes are showed by lymphoscintigraphy, they are dyed by patent blue and identified by intraoperative gamma probe detection. It isn t necessary to use magnification for the procedure. The model demonstrated that sentinel node biopsy in rats is feasible. So, besides allowing researches in this field, the model is useful for training and diffusing this technique.
  • Imagem de Miniatura
    Item
    Acesso aberto (Open Access)
    Sentinel lymph node biopsy in rats. Comparison between paraffin and frozen section analysis
    (Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2014-01-01) Santos Filho, Ivan Dunshee de Abranches Oliveira [UNIFESP]; Enokihara, Mílvia Maria Simões e Silva [UNIFESP]; Shie, Janaina Namba [UNIFESP]; Oliveira, Andrea Fernandes de [UNIFESP]; Santos, Ivan Dunshee de Abranches Oliveira [UNIFESP]; Ferreira, Lydia Masako [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    PURPOSE:To simulate a lymph node metastasis in an animal model using activated carbon, assess their identification in frozen section analysis and compare with histopathological examination in paraffin.METHODS:Thirty two adult female rats were used. They received the carbon injection on its hind legs. Half of the rats was sacrificed on day one, and the other half after 21 days. Thus, 64 lymph nodes were dissected and split longitudinally. One half of the lymph node was sent immediately to frozen section analysis. The other half was fixed in 10% formaldehyde to be cut in paraffin. Slides were divided into quadrants and classified by the presence of carbon in these four quadrants_ They were also classified by the carbon staining intensity.RESULTS:Comparing the slides obtained in the first day and 21 days, there was a tendency of carbon to spread over time, but without statistical significance. The intensity did not alter over time.CONCLUSION:There was no concordance between the two methods of pathological analysis, however the actived carbon was seen in all lymph nodes.