Navegando por Palavras-chave "Litopenaeus vannamei"
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- ItemSomente Metadadados2,3-Di-O-sulfo glucuronic acid: An unmodified and unusual residue in a highly sulfated chondroitin sulfate from Litopenaeus vannamei(Elsevier Sci Ltd, 2018) Cavalcante, Romulo S.; Brito, Adriana S.; Palhares, Lais C. G. F.; Lima, Marcelo A. [UNIFESP]; Cavalheiro, Renan P. [UNIFESP]; Nader, Helena B. [UNIFESP]; Sassaki, Guilherme L.; Chavante, Suely F.The occurrence of a natural and unmodified highly sulfated chondroitin sulfate from Litopenaeus vannamei heads (sCS) is herein reported. Its partial digestion by Chondroitinases AC and ABC together with its electrophoretic migration profile revealed it as a highly sulfated chondroitin sulfate despite its average molecular weight being similar to CSA. Using orthogonal 1D/2D NMR experiments, the anomeric signals (delta 4.62/106.0) corresponding to unusual 2,3-di-O-Sulfo-GlcA (similar to 36%), U3(3S) (delta 4.42/84.1, similar to 63%) and U2(2S) (4.12/80.1, similar to 50%) substitutions were confirmed. In addition, non-sulfated GlcA (delta 4.5/106.3) linked to 4-O-(A1(4S), 36%) or 6-O-Sulfo (A1(6S), 28%) GalNAc (delta 4.64/103.5) was observed. Although the biological role of sCS in shrimp is unknown, its influence on hemostasis was also demonstrated. The sCS identification brings to light new questions about the hierarchical model of GAGs biosynthesis and contributes to the better understanding of the subtle relationship between GAGs structure and function.
- ItemSomente MetadadadosAnti-inflammatory properties of a heparin-like glycosaminoglycan with reduced anti-coagulant activity isolated from a marine shrimp(Elsevier B.V., 2008-11-01) Brito, Adriana S.; Arimateia, Dayse S.; Souza, Lucilla R.; Lima, Marcelo A.; Santos, Vanessa O.; Medeiros, Valquiria P. [UNIFESP]; Ferreira, Paula A.; Silva, Rodrigo A.; Ferreira, Carmen V.; Justo, Giselle Z. [UNIFESP]; Leite, Edda L.; Andrade, Giulianna P. V.; Oliveira, Fernanda W.; Nader, Helena B. [UNIFESP]; Chavante, Suely F.; Univ Fed Rio Grande do Norte; Universidade Federal de São Paulo (UNIFESP); Universidade Estadual de Campinas (UNICAMP)The anti-inflammatory properties of a heparin-like compound from the shrimp Litopenaeus vannamei are related. Besides reducing significantly (p < 0.001) the influx of inflammatory cells to injury site in a model of acute inflammation, shrimp heparin-like compound was able to reduce the matrix metalloproteinase (MMPs) activity in the peritoneal lavage of inflamed animals. Moreover, this compound also reduced almost 90% the activity of MMP-9 secreted by human activated leukocytes. Negligible anti-coagulant activities in aPPT assay and a poor bleeding potential make this compound a better alternative than mammalian heparin as a possible anti-inflammatory drug. (C) 2008 Elsevier B.V. All rights reserved.
- ItemSomente MetadadadosA comparison between water exchange and settling tank as a method for suspended solids management in intensive biofloc technology systems: effects on shrimp (Litopenaeus vannamei) performance, water quality and water use(Wiley, 2017) Arantes, Rafael; Schveitzer, Rodrigo [UNIFESP]; Magnotti, Caio; Lapa, Katt Regina; Vinatea, LuisBiofloc systems rely on microbial processes in the water column to recycle animal waste products, reducing the need for water exchange. These increases biofloc concentration in the water and some form of removal is needed. An experiment was carried out to evaluate two management practices to control biofloc in Litopenaeus vannamei culture. Six tanks (48 m(3)) were divided into two treatments: water exchange and solid settler. Shrimp were stocked at 164 shrimp m(-2) and with 0.67 g of weight. After 61 days, shrimp under solid settler treatment demonstrated mean weight of 12.7 +/- 0.5 g with survival of 73.8 +/- 1.4%, and those under water exchange had a final weight of 10.1 +/- 0.2 g and survival rate of 57.8 +/- 11.1%. Total suspended solids did not differ between the treatments: 326.8 +/- 24.9 mg L-1 for water exchange and 310.9 +/- 25.3 mg L-1 for solid settlers. Settleable solids and productivity/respiration ratio was higher (P < 0.05) in water exchange treatment, indicating differences in physical and biological characteristics of bioflocs. Solids removal method influenced the water use, in which 1150 +/- 249 L of water was necessary to produce one kilogram of shrimp using water exchange strategy, and 631 +/- 25 L kg(-1) with the use of settlers. Our results indicate that continuous operation of settlers can reduce variability in solids characteristics and water quality variables such as ammonia. Both strategies are efficient in controlling biofloc concentrations of the water
- ItemSomente MetadadadosNutrient discharge, sludge quantity and characteristics in biofloc shrimp culture using two methods of carbohydrate fertilization(Elsevier Sci Ltd, 2017) Arantes, Rafael; Schveitzer, Rodrigo [UNIFESP]; Seiffert, Walter Quadros; Lapa, Katt Regina; Vinatea, LuisThe aim of this study was to evaluate the effect of two methodologies of carbohydrate fertilization on the volume and characteristics of effluent from intensive biofloc shrimp cultivation. Six fiberglass circular tanks (50 m(2) each) were divided into two treatments. In the treatment called continuous (CONT), the tanks received daily molasses fertilization throughout the entire rearing period. In the treatment named initial (INI), molasses was used only in the early weeks of cultivation. Juvenile Litopenaeus vannamei (0.87 +/- 0.10 g) were stocked at a density of 180 animals m(-2) and cultured during 12 weeks until they reached an average weight of 12 g. The tanks were operated with no water exchange and the total suspended solids concentration were kept between 300 and 400 mg L-1 using settling chambers. The sludge produced and the wastewater at harvest were quantified and their characteristics were determined. The production of TSS in the CONT treatment was higher (0.25 kg of solids per kg of applied feed) than in the INI treatment (0.16 kg kg(-1)) (P< 0.05). The analysis of the sludge revealed a high amount of volatile solids in both treatments, between 636 and 702 g kg(-1). However, due to the elevated sludge nitrogen content, the carbon to nitrogen (C:N) ratio was low, with values of 6.4 +/- 1.4 and 7.5 +/- 1.6 for INI and CONT respectively. The BOD:TSS ratio was also low in both treatments, but the INI showed lower values (10.3 +/- 0.6%) than the CONT (14.9 +/- 0.0%) (P<0.05). Both fertilization strategies were able to modify the characteristics of sludge produced during cultivation. Moreover, the high nitrogen and sulfate content of the sludge in both treatments indicated that it may be difficult to use an anaerobic digestion process to treat sludge. In the INI treatment tanks, the sludge is partially stabilized, while in the CONT there was a greater need for stabilization. (C) 2016 Elsevier B.V. All rights reserved.
- ItemAcesso aberto (Open Access)Recovery of protein, chitin, carotenoids and glycosaminoglycans from Pacific white shrimp (Litopenaeus vannamei) processing waste(Elsevier B.V., 2012-04-01) Cahú, Thiago Barbosa; Santos, Suzan Diniz; Mendes, Aline [UNIFESP]; Cóordula, Carolina Ribeiro [UNIFESP]; Chavante, Suely Ferreira [UNIFESP]; Carvalho, Luiz Bezerra de; Nader, Helena Bonciani [UNIFESP]; Bezerra, Ranilson de Souza; Universidade Federal de Pernambuco (UFPE); Universidade Federal de São Paulo (UNIFESP); Univ Fed Rio Grande do NorteShrimp head waste is a major byproduct of crustacean processing in North-eastern Brazil and represents an interesting source of bioactive molecules. Additionally, its use increases the sustainability of processing fishery products. the present study reports a process developed for recovering bioactive molecules from shrimp heads through autolysis. A protein hydrolysate (120 +/- 0.4 g) formed by a 9% (w/v) solution was recovered and lyophilized from I kg of shrimp heads. Approximately 195 +/- 0.5 mg of carotenoids was recovered as an ethanolic extract. the recovery of chitin and chitosan were 25 +/- 2 g kg(-1) and 17 +/- 4 g kg(-1) wet processing waste, respectively. Chitosans were characterized by C-13 NMR, and FT-IR analysis and exhibited a variable degree of deacetylation (60-80%). Sulfated glycosaminoglycans that exhibited electrophoretic migration similar to mammalian standards were also recovered (79 +/- 2 mg kg(-1) wet processing waste), and their degradation products suggested the presence of C6-sulfated heparan sulfate. These data point to the feasibility of an integrated process for isolating highly bioactive molecules, such as sulfated- and amino-polysaccharides, with a broad spectrum of applications from shrimp processing waste. (C) 2011 Elsevier B.V. All rights reserved.